Recombinant Mouse Synaptotagmin VII Protein Standard (His tag)
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Recombinant Mouse Synaptotagmin VII Protein Standard (His tag) is a Mouse Fragment protein, expressed in Escherichia coli, with >80%, suitable for sELISA, SDS-PAGE.
View Alternative Names
Synaptotagmin-7, Synaptotagmin VII, SytVII
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Mouse Synaptotagmin VII Protein Standard (His tag) (AB323160)
Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Mouse Synaptotagmin VII Protein Standard (His tag) (AB323160)
SDS-PAGE analysis of ab323160 under reducing conditions for 2ug protein.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
General info
Function
Ca(2+) sensor involved in Ca(2+)-dependent exocytosis of secretory and synaptic vesicles through Ca(2+) and phospholipid binding to the C2 domain. Ca(2+) induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. SYT7 binds Ca(2+) with high affinity and slow kinetics compared to other synaptotagmins (PubMed : 26738595). Involved in Ca(2+)-triggered lysosomal exocytosis, a major component of the plasma membrane repair (By similarity). Ca(2+)-regulated delivery of lysosomal membranes to the cell surface is also involved in the phagocytic uptake of particles by macrophages (PubMed : 16982801, PubMed : 21041449). Ca(2+)-triggered lysosomal exocytosis also plays a role in bone remodeling by regulating secretory pathways in osteoclasts and osteoblasts (PubMed : 18539119). Involved in cholesterol transport from lysosome to peroxisome by promoting membrane contacts between lysosomes and peroxisomes : probably acts by promoting vesicle fusion by binding phosphatidylinositol-4,5-bisphosphate on peroxisomal membranes (PubMed : 25860611). Acts as a key mediator of synaptic facilitation, a process also named short-term synaptic potentiation : synaptic facilitation takes place at synapses with a low initial release probability and is caused by influx of Ca(2+) into the axon terminal after spike generation, increasing the release probability of neurotransmitters (PubMed : 24569478, PubMed : 26738595). Probably mediates synaptic facilitation by directly increasing the probability of release (PubMed : 26738595). May also contribute to synaptic facilitation by regulating synaptic vesicle replenishment, a process required to ensure that synaptic vesicles are ready for the arrival of the next action potential : SYT7 is required for synaptic vesicle replenishment by acting as a sensor for Ca(2+) and by forming a complex with calmodulin (PubMed : 24569478). Also acts as a regulator of Ca(2+)-dependent insulin and glucagon secretion in beta-cells (PubMed : 18308938, PubMed : 19171650). Triggers exocytosis by promoting fusion pore opening and fusion pore expansion in chromaffin cells (PubMed : 20956309). Also regulates the secretion of some non-synaptic secretory granules of specialized cells (By similarity).
Sequence similarities
Belongs to the synaptotagmin family.
Post-translational modifications
Palmitoylated at its vesicular N-terminus; palmitoylation is required for localization to lysosome and phagocytosis in macrophages.
Subcellular localisation
Lysosome membrane
Target data
Product promise
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