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AB276866

Recombinant Mouse SynCAM/CADM1 protein (His tag)

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Recombinant Mouse SynCAM/CADM1 protein (His tag) is a Mouse Fragment protein, in the 1 to 377 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Igsf4, Necl2, Ra175, Syncam, SynCam1, Tslc1, Cadm1, Cell adhesion molecule 1, Immunoglobulin superfamily member 4, Nectin-like protein 2, Spermatogenic immunoglobulin superfamily, Synaptic cell adhesion molecule, Tumor suppressor in lung cancer 1, IgSF4, NECL-2, SgIgSF, SynCAM, TSLC-1

1 Images
SDS-PAGE - Recombinant Mouse SynCAM/CADM1 protein (His tag) (AB276866)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse SynCAM/CADM1 protein (His tag) (AB276866)

SDS-PAGE analysis of ab276866

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8R5M8

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB316889

Human SynCAM/CADM1 ELISA Kit

SimpleStep

Sandwich ELISA - Human SynCAM/CADM1 ELISA Kit (AB316889)

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Proteins & Peptides

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASAVLPSGSQCAAAAAVAAAAAPPGLRLRLLLLLLSAAALIPTGDGQNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNKELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDAFELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGTIGAVDH","proteinLength":"Fragment","predictedMolecularWeight":"38.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":377,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8R5M8","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SynCAM also known as CADM1 (Cell Adhesion Molecule 1) is a protein that plays an important role in cell adhesion processes. It has an approximate mass of 105 kDa. SynCAM/CADM1 is expressed in various tissues including the brain where it supports synapse formation and maintenance. It is a member of the immunoglobulin superfamily and contains several I-set V-set and C2-set domains which contribute to its adhesion properties.
Biological function summary

SynCAM/CADM1 acts as a cell recognition molecule participating in the formation of synaptic connections in neural circuits. It is a component of a complex that regulates synaptic clustering and neural connectivity. In the nervous system SynCAM/CADM1 facilitates the initial axon-dendrite contact allowing efficient synaptic communication. Its expression and interaction affect the building of mature and functional synapses which are essential for cognitive processes.

Pathways

SynCAM/CADM1 integrates into synaptic development and cell signaling pathways. It interacts with scaffolding proteins like PSD-95 which stabilize the synaptic structure. The protein also participates in the Ras signaling pathway influencing cell growth and survival signals. Through its engagement in these pathways SynCAM/CADM1 plays a role in neural circuit plasticity and function maintaining proper communication between neurons.

SynCAM/CADM1 shows connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysregulation is associated with abnormal neural connectivity leading to impaired cognitive function. SynCAM/CADM1's interaction with the L1CAM protein has been implicated in these disorders as both are involved in neural adhesion and signaling pathways that when altered can contribute to the development of such conditions.
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Specifications

Form

Lyophilized

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General info

Function

Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner (PubMed : 12202822, PubMed : 12799182, PubMed : 12826663). Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner (PubMed : 12826663). Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed : 15811952). In mast cells, may mediate attachment to and promote communication with nerves (By similarity). CADM1, together with MITF, is essential for development and survival of mast cells in vivo (PubMed : 15158462, PubMed : 16605125). By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node (PubMed : 19752223). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (PubMed : 24687959). Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (PubMed : 24687959). Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (PubMed : 12202822, PubMed : 23209303). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons (PubMed : 15707673). May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa (PubMed : 12606335, PubMed : 16612000).

Sequence similarities

Belongs to the nectin family.

Post-translational modifications

N-glycosylated.. Glycosylation at Asn-70 and Asn-104 promotes adhesive binding and synapse induction.

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Product protocols

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Target data

Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner (PubMed : 12202822, PubMed : 12799182, PubMed : 12826663). Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner (PubMed : 12826663). Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed : 15811952). In mast cells, may mediate attachment to and promote communication with nerves (By similarity). CADM1, together with MITF, is essential for development and survival of mast cells in vivo (PubMed : 15158462, PubMed : 16605125). By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node (PubMed : 19752223). Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (PubMed : 24687959). Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (PubMed : 24687959). Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly (PubMed : 12202822, PubMed : 23209303). May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons (PubMed : 15707673). May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa (PubMed : 12606335, PubMed : 16612000).
See full target information Cadm1
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