Recombinant Mouse Tau protein (His tag) is a Mouse Full Length protein, in the 2 to 733 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
A D P R Q E F D T M E D H A G D Y T L L Q D Q E G D M D H G L K E S P P Q P P A D D G A E E P G S E T S D A K S T P T A E D V T A P L V D E R A P D K Q A A A Q P H T E I P E G I T A E E A G I G D T P N Q E D Q A A G H V T Q G R R E G Q A P D L G T S D W T R Q Q V S S M S G A P L L P Q G L R E A T C Q P S G T R P E D I E K S H P A S E L L R R G P P Q K E G W G Q D R L G S E E E V D E D L T V D E S S Q D S P P S Q A S L T P G R A A P Q A G S G S V C G E T A S V P G L P T E G S V P L P A D F F S K V S A E T Q A S Q P E G P G T G P M E E G H E A A P E F T F H V E I K A S T P K E Q D L E G A T V V G V P G E E Q K A Q T Q G P S V G K G T K E A S L Q E P P G K Q P A A G L P G R P V S R V P Q L K A R V A S K D R T G N D E K K A K T S T P S C A K A P S H R P C L S P T R P T L G S S D P L I K P S S P A V S P E P A T S P K H V S S V T P R N G S P G T K Q M K L K G A D G K T G A K I A T P R G A A S P A Q K G T S N A T R I P A K T T P S P K T P P G S G E P P K S G E R S G Y S S P G S P G T P G S R S R T P S L P T P P T R E P K K V A V V R T P P K S P S A S K S R L Q T A P V P M P D L K N V R S K I G S T E N L K H Q P G G G K V Q I I N K K L D L S N V Q S K C G S K D N I K H V P G G G S V Q I V Y K P V D L S K V T S K C G S L G N I H H K P G G G Q V E V K S E K L D F K D R V Q S K I G S L D N I T H V P G G G N K K I E T H K L T F R E N A K A K T D H G A E I V Y K S P V V S G D T S P R H L S N V S S T G S I D M V D S P Q L A T L A D E V S A S L A K Q G L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Alternative names
Mtapt, Tau, Mapt, Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau
Recommended products
Recombinant Mouse Tau protein (His tag) is a Mouse Full Length protein, in the 2 to 733 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P10637-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A D P R Q E F D T M E D H A G D Y T L L Q D Q E G D M D H G L K E S P P Q P P A D D G A E E P G S E T S D A K S T P T A E D V T A P L V D E R A P D K Q A A A Q P H T E I P E G I T A E E A G I G D T P N Q E D Q A A G H V T Q G R R E G Q A P D L G T S D W T R Q Q V S S M S G A P L L P Q G L R E A T C Q P S G T R P E D I E K S H P A S E L L R R G P P Q K E G W G Q D R L G S E E E V D E D L T V D E S S Q D S P P S Q A S L T P G R A A P Q A G S G S V C G E T A S V P G L P T E G S V P L P A D F F S K V S A E T Q A S Q P E G P G T G P M E E G H E A A P E F T F H V E I K A S T P K E Q D L E G A T V V G V P G E E Q K A Q T Q G P S V G K G T K E A S L Q E P P G K Q P A A G L P G R P V S R V P Q L K A R V A S K D R T G N D E K K A K T S T P S C A K A P S H R P C L S P T R P T L G S S D P L I K P S S P A V S P E P A T S P K H V S S V T P R N G S P G T K Q M K L K G A D G K T G A K I A T P R G A A S P A Q K G T S N A T R I P A K T T P S P K T P P G S G E P P K S G E R S G Y S S P G S P G T P G S R S R T P S L P T P P T R E P K K V A V V R T P P K S P S A S K S R L Q T A P V P M P D L K N V R S K I G S T E N L K H Q P G G G K V Q I I N K K L D L S N V Q S K C G S K D N I K H V P G G G S V Q I V Y K P V D L S K V T S K C G S L G N I H H K P G G G Q V E V K S E K L D F K D R V Q S K I G S L D N I T H V P G G G N K K I E T H K L T F R E N A K A K T D H G A E I V Y K S P V V S G D T S P R H L S N V S S T G S I D M V D S P Q L A T L A D E V S A S L A K Q G L
- Accession
- P10637
- Protein length
- Full Length
- Predicted molecular weight
- 78.1 kDa
- Amino acids
- 2 to 733
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Post-translational modifications
Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Tau also known as microtubule-associated protein Tau (MAPT) plays an important role in stabilizing microtubules in neuronal cells. Tau is primarily found in the central nervous system but also exists in peripheral neurons. Human Tau protein comes in six isoforms due to alternative splicing with molecular weights ranging from 48 kDa to 67 kDa. This protein predominantly locates in the axons of neurons where it maintains the stability of microtubule tracks necessary for axonal transport.
- Biological function summary
Tau is involved in the assembly and stabilization of microtubules essential for maintaining neuronal structure. It interacts with microtubule-binding domains (MBD) to bind and bundle microtubules facilitating intracellular transport. Tau forms a part of the neuronal cytoskeleton complex working closely with other cytoskeletal proteins to preserve the proper axonal transport and function. Abnormally phosphorylated Tau often termed phospho-Tau disrupts this complex affecting microtubule stability.
- Pathways
Tau has critical involvement in several signaling cascades such as the microtubule-binding and transport pathways. Glycogen synthase kinase 3 beta (GSK3β) and cyclin-dependent kinase 5 (CDK5) frequently phosphorylate Tau controlling its interaction with microtubules. Phosphorylated Tau accumulates leading to the formation of neurofibrillary tangles often observed in neurodegenerative conditions. Additionally Tau interacts with GAPDH impacting cellular energy regulation through potential pathway cross-talk involving oxidative stress responses.
- Associated diseases and disorders
Tau is closely associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease hyperphosphorylated Tau aggregates into paired helical filaments forming neurofibrillary tangles while similar aggregates are observed in frontotemporal dementia. In these conditions Tau links to amyloid precursor protein (APP) where misregulated phosphorylation-driven interactions contribute to neurodegeneration. Identifying phospho-Tau and its altered interactions with related proteins aids in understanding and potentially treating these disorders.
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3 product images
SDS-PAGE - Recombinant Mouse Tau protein (His tag) (ab241263)
Analysis of ab241263 by (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Mouse Tau protein (His tag) (ab241263)
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241263 could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Mapt.
Mass Spectrometry - Recombinant Mouse Tau protein (His tag) (ab241263)
Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241263 could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Mapt.
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