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AB276989

Recombinant mouse TCblR protein (His tag)

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Recombinant mouse TCblR protein (His tag) is a Mouse Fragment protein, in the 1 to 208 aa range, expressed in HEK 293 cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

CD320, CD320 antigen, Transcobalamin receptor, TCblR, Cd320

2 Images
Functional Studies - Recombinant mouse TCblR protein (His tag) (AB276989)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse TCblR protein (His tag) (AB276989)

Measured by its binding ability in a functional ELISA.

Immobilized mouse CD320-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse TCN2-His.

The EC50 of biotinylated mouse TCN2-His is 0.10-0.24 μg/mL.

SDS-PAGE - Recombinant mouse TCblR protein (His tag) (AB276989)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant mouse TCblR protein (His tag) (AB276989)

SDS-PAGE analysis of ab276989

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA.

Immobilized mouse CD320-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse TCN2-His.

The EC50 of biotinylated mouse TCN2-His is 0.10-0.24 μg/mL.

Accession

Q9Z1P5

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MARGGAGRAVALGLVLRLLFGLRTGLEAAPAPAHTRVQVSGSRADSCPTDTFQCLTSGYCVPLSWRCDGDQDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNLNCSRPPCQESELHCILDDVCIPHTWRCDGHPDCLDSSDELSCDTDTEIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYG","proteinLength":"Fragment","predictedMolecularWeight":"21 kDa","actualMolecularWeight":null,"aminoAcidEnd":208,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The transcobalamin receptor (TCblR) also known as CD320 is a membrane protein involved in the uptake of vitamin B12 (cobalamin) by cells. TCblR is recognized for its role in the binding and internalization of transcobalamin-bound vitamin B12 enabling this essential vitamin to enter cells. The protein has a molecular mass of approximately 50 kDa. TCblR is expressed on the surface of many cell types with notable expression in bone marrow lymphoid tissues and certain tumor cells.
Biological function summary

TCblR facilitates the cellular uptake of vitamin B12 which is necessary for DNA synthesis and cell growth. It is not part of a larger protein complex but it interacts directly with transcobalamin a carrier protein that escorts vitamin B12 through the bloodstream. The interaction between TCblR and transcobalamin-bound vitamin B12 permits the transport of this vitamin into the cell where it plays a role in metabolic processes.

Pathways

TCblR plays an important role in the cobalamin metabolism pathway by mediating the internalization of vitamin B12 into cells. This uptake is important for the methionine and folate cycles where vitamin B12 functions as a cofactor. TCblR is therefore linked to the methionine synthase enzyme which is part of the folate cycle and necessary for the conversion of homocysteine to methionine.

Mutations or deficiencies in TCblR function can lead to conditions like megaloblastic anemia and neuropathy due to vitamin B12 deficiency. Altered expression of TCblR has been associated with certain types of cancer where it can influence cell proliferation by modulating vitamin B12 uptake. Its interaction with the transcobalamin protein highlights its relevance in maintaining B12 levels and cellular health.
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Specifications

Form

Lyophilized

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General info

Function

Receptor for transcobalamin saturated with cobalamin (TCbl). Plays an important role in cobalamin uptake. Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells. Functions as a costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation. Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10.

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Product protocols

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Target data

Receptor for transcobalamin saturated with cobalamin (TCbl). Plays an important role in cobalamin uptake. Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells. Functions as a costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation. Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC). CD320 augments the proliferation of PC precursors generated by IL-10.
See full target information Cd320
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