Recombinant Mouse Telomerase reverse transcriptase protein (Tagged) is a Mouse Fragment protein, in the 595 to 928 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
E V R H H Q D T W L A M P I C R L R F I P K P N G L R P I V N M S Y S M G T R A L G R R K Q A Q H F T Q R L K T L F S M L N Y E R T K H P H L M G S S V L G M N D I Y R T W R A F V L R V R A L D Q T P R M Y F V K A D V T G A Y D A I P Q G K L V E V V A N M I R H S E S T Y C I R Q Y A V V R R D S Q G Q V H K S F R R Q V T T L S D L Q P Y M G Q F L K H L Q D S D A S A L R N S V V I E Q S I S M N E S S S S L F D F F L H F L R H S V V K I G D R C Y T Q C Q G I P Q G S S L S T L L C S L C F G D M E N K L F A E V Q R D G L L L R F V D D F L L V T P H L D Q A K T F L S T L V H G V P E Y G C M I N L Q K T V V N F P V E P G T L G G A A P Y Q L P A H C L F P W C G L L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).
Alternative names
Telomerase reverse transcriptase, Telomerase catalytic subunit, Tert
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Recombinant Mouse Telomerase reverse transcriptase protein (Tagged) is a Mouse Fragment protein, in the 595 to 928 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O70372-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- E V R H H Q D T W L A M P I C R L R F I P K P N G L R P I V N M S Y S M G T R A L G R R K Q A Q H F T Q R L K T L F S M L N Y E R T K H P H L M G S S V L G M N D I Y R T W R A F V L R V R A L D Q T P R M Y F V K A D V T G A Y D A I P Q G K L V E V V A N M I R H S E S T Y C I R Q Y A V V R R D S Q G Q V H K S F R R Q V T T L S D L Q P Y M G Q F L K H L Q D S D A S A L R N S V V I E Q S I S M N E S S S S L F D F F L H F L R H S V V K I G D R C Y T Q C Q G I P Q G S S L S T L L C S L C F G D M E N K L F A E V Q R D G L L L R F V D D F L L V T P H L D Q A K T F L S T L V H G V P E Y G C M I N L Q K T V V N F P V E P G T L G G A A P Y Q L P A H C L F P W C G L L L
- Accession
- O70372
- Protein length
- Fragment
- Predicted molecular weight
- 54.1 kDa
- Amino acids
- 595 to 928
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis (By similarity).
- Sequence similarities
Belongs to the reverse transcriptase family. Telomerase subfamily.
- Post-translational modifications
Phosphorylation at Tyr-697 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location. Phosphorylation at the G2/M phase at Ser-447 by DYRK2 promotes ubiquitination by the EDVP complex and degradation (By similarity).
- Subcellular localisation
- Nucleus, Nucleolus, Nucleoplasm, PML body
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Telomerase reverse transcriptase also known as TERT is an enzyme with a molecular mass of about 127 kDa. It functions as a catalytic subunit of the telomerase complex which extends telomeres by adding the nucleotide sequence "TTAGGG" to the ends of chromosomes. TERT operates with a telomerase RNA component (TERC) to synthesize DNA using an RNA template a process called reverse transcription. TERT is expressed in embryonic tissues adult stem cells and cancer cells where rapid cell division occurs.
- Biological function summary
TERT plays an important role in maintaining telomere length essential for protecting chromosome stability. The protein forms part of the telomerase holoenzyme complex working together with TERC to perform its function. Telomerase activity allows cells to bypass the Hayflick limit preventing senescence and enhancing replication potential. In addition to telomere maintenance TERT influences gene regulation and cellular proliferation.
- Pathways
TERT holds significance in the telomere maintenance and signaling pathways. Telomere maintenance ensures genomic integrity and cellular longevity a process fundamental for tissue homeostasis and regeneration. TERT interacts with proteins such as dyskerin and TERC within these pathways. Its regulatory roles in these pathways also reveal interactions with other key proteins like hypothesized targets in the DNA damage response.
- Associated diseases and disorders
The upregulation of TERT is associated with cancer and degenerative diseases. Increased telomerase activity is a common feature in many cancers leading to uncontrolled cell proliferation. Additionally mutations in TERT and its associated components can contribute to disorders like dyskeratosis congenita a rare genetic condition resulting in bone marrow failure. In these conditions TERT interaction with proteins involved in chromosomal maintenance and repair becomes important for understanding disease mechanisms.
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1 product image
SDS-PAGE - Recombinant Mouse Telomerase reverse transcriptase protein (Tagged) (ab226420)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226420 with 5% enrichment gel and 15% separation gel.
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