Recombinant Mouse Thrombopoietin protein (Active) is a Mouse Fragment protein, in the 22 to 356 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
S P V A P A C D P R L L N K L L R D S H L L H S R L S Q C P D V D P L S I P V L L P A V D F S L G E W K T Q T E Q S K A Q D I L G A V S L L L E G V M A A R G Q L E P S C L S S L L G Q L S G Q V R L L L G A L Q G L L G T Q L P L Q G R T T A H K D P N A L F L S L Q Q L L R G K V R F L L L V E G P T L C V R R T L P T T A V P S S T S Q L L T L N K F P N R T S G L L E T N F S V T A R T A G P G L L S R L Q G F R V K I T P G Q L N Q T S R S P V Q I S G Y L N R T H G P V N G T H G L F A G T S L Q T L E A S D I S P G A F N K G S L A F N L Q G G L P P S P S L A P D G H T P F P P S P A L P T T H G S P P Q L H P L F P D P S T T M P N S T A P H P V T M Y P H P R N L S Q E T
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Alternative names
Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, MGDF, Thpo
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Recombinant Mouse Thrombopoietin protein (Active) is a Mouse Fragment protein, in the 22 to 356 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by dose dependent proliferation of MO7e cells.
ED50 is ≤ 9.2 ng/ml, corresponding to a specific activity of 1.09 x 105 units/mg.
- Accession
- P40226-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- S P V A P A C D P R L L N K L L R D S H L L H S R L S Q C P D V D P L S I P V L L P A V D F S L G E W K T Q T E Q S K A Q D I L G A V S L L L E G V M A A R G Q L E P S C L S S L L G Q L S G Q V R L L L G A L Q G L L G T Q L P L Q G R T T A H K D P N A L F L S L Q Q L L R G K V R F L L L V E G P T L C V R R T L P T T A V P S S T S Q L L T L N K F P N R T S G L L E T N F S V T A R T A G P G L L S R L Q G F R V K I T P G Q L N Q T S R S P V Q I S G Y L N R T H G P V N G T H G L F A G T S L Q T L E A S D I S P G A F N K G S L A F N L Q G G L P P S P S L A P D G H T P F P P S P A L P T T H G S P P Q L H P L F P D P S T T M P N S T A P H P V T M Y P H P R N L S Q E T
- Accession
- P40226
- Protein length
- Fragment
- Predicted molecular weight
- 35.65 kDa
- Amino acids
- 22 to 356
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
- Sequence similarities
Belongs to the EPO/TPO family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombopoietin also known as TPO is an important glycoprotein hormone with a mass of about 70 kDa. It is primarily produced in the liver but can also be found in the kidneys and bone marrow stromal cells. Thrombopoietin binds to and activates its receptor c-Mpl which is present on hematopoietic progenitor cells. This protein has a significant role in regulating the production of blood platelets by stimulating the proliferation and differentiation of megakaryocytes the precursor cells for platelets.
- Biological function summary
Thrombopoietin functions in hematopoiesis by supporting the maturation of megakaryocytes. It operates independently but can interact with other factors to optimize platelet production. Although it does not form a part of a large protein complex it is essential for the proper function of hematopoietic progenitors across several stages of differentiation. Its activity ensures the maintenance of platelet levels in the bloodstream therefore preventing conditions like thrombocytopenia.
- Pathways
Thrombopoietin plays a critical role in the megakaryocyte/platelet lineage pathway. It directly influences the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway which is vital for driving the cell cycle and promoting cell survival. Thrombopoietin shares a functional connection with EPO (erythropoietin) which is involved in red blood cell production although they act on different lineage-specific progenitors.
- Associated diseases and disorders
Thrombopoietin is intricately linked to conditions like thrombocytopenia where there are abnormally low platelet counts and thrombocythemia characterized by elevated platelet levels. Dysregulation of Thrombopoietin production or its receptor activity can lead to these disorders. It has a relationship with other hematopoietic growth factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which can also influence the disorders of blood cell lines. Understanding its signaling pathways provides insights into therapeutic targets for diseases involving platelet imbalances.
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2 product images
SDS-PAGE - Recombinant Mouse Thrombopoietin protein (Active) (ab282408)
SDS-page analysis of ab282408.
Migration on the SDS-PAGE gel is due to extensive glycosylation.
Biological Activity - Recombinant Mouse Thrombopoietin protein (Active) (ab282408)
Fully biologically active determined by dose dependent proliferation of MO7e cells.
ED50 is ≤ 9.2 ng/ml, corresponding to a specific activity of 1.09 x 105 units/mg.
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