Recombinant mouse TIGIT protein (His tag) is a Mouse Fragment protein, in the 26 to 143 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
G T I D T K R N I S A E E G G S V I L Q C H F S S D T A E V T Q V D W K Q Q D Q L L A I Y S V D L G W H V A S V F S D R V V P G P S L G L T F Q S L T M N D T G E Y F C T Y H T Y P G G I Y K G R I F L K V Q E S S V A Q F Q T A P L G G T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Alternative names
T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3, Vstm3, Tigit
Recommended products
Recombinant mouse TIGIT protein (His tag) is a Mouse Fragment protein, in the 26 to 143 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names
T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3, Vstm3, Tigit
Key facts
- Purity
>90% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Immobilized ab224632 at 5 μg/mL (100 μL/well) can bind Mouse CD155, Mouse IgG2a Fc Tag with a linear range of 5-78 ng/mL.
- Accession
- P86176-1
- Animal free
No
- Species
Mouse
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 5% Trehalose
Sequence info
Amino acid sequence
G T I D T K R N I S A E E G G S V I L Q C H F S S D T A E V T Q V D W K Q Q D Q L L A I Y S V D L G W H V A S V F S D R V V P G P S L G L T F Q S L T M N D T G E Y F C T Y H T Y P G G I Y K G R I F L K V Q E S S V A Q F Q T A P L G G T
- Accession
- Protein length
Fragment
- Predicted molecular weight
14.8 kDa
- Amino acids
26 to 143
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
After reconstitution this product is stable for 3 months at -70°C under sterile conditions.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
- Biological function summary
TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.
- Pathways
The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.
- Associated diseases and disorders
TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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2 product images
Functional Studies - Recombinant mouse TIGIT protein (His tag) (ab224632)
Immobilized ab224632 at 5 μg/mL (100 μL/well) can bind Mouse CD155, Mouse IgG2a Fc Tag with a linear range of 5-78 ng/mL.
SDS-PAGE - Recombinant mouse TIGIT protein (His tag) (ab224632)
ab224632 analyzed by SDS-PAGE under reducing conditions and stained overnight with Coomassie Blue.
The protein migrates as 18-30 kDa under reducing conditions (SDS-PAGE) due to glycosylation.
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