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Recombinant Mouse Tim 2 protein (Fc Chimera) is a Mouse Fragment protein, in the 22 to 229 aa range, expressed in HEK 293, with >98% purity, < 5 EU/mg endotoxin level and suitable for SDS-PAGE.

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Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 5 EU/mg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

H T A V Q G L A G H P V T L P C I Y S T H L G G I V P M C W G L G E C R H S Y C I R S L I W T N G Y T V T H Q R N S L Y Q L K G N I S E G N V S L T I E N T V V G D G G P Y C C V V E I P G A F H F V D Y M L E V K P E I S T S P P T R P T A T G R P T T I S T R S T H V P T S T R V S T S T S P T P A H T E T Y K P E A T T F Y P D Q T T A E V T E T L P D T P A D W H N T V T S S D D P W D D N T E V I P P Q K P Q K N L N

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Cell surface glycoprotein that participates in iron homeostasis in the liver, the kidney, the retina and oligodendrocytes by acting as a receptor of H-ferritin (PubMed:16203866, PubMed:33065090). Mechanistically, mediates iron-containing ferritin uptake via an endocytic pathway, trafficking to endosomes and subsequently to lysosomes (PubMed:16043519, PubMed:21886823). Plays also an important role in the regulation of Th2 immunity (PubMed:16043519). Receptor for SEMA4A involved in the regulation of T-cell function, enhancing T-cell activation (PubMed:12374982).

Alternative names

Recommended products

Recombinant Mouse Tim 2 protein (Fc Chimera) is a Mouse Fragment protein, in the 22 to 229 aa range, expressed in HEK 293, with >98% purity, < 5 EU/mg endotoxin level and suitable for SDS-PAGE.

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 5 EU/mg

Expression system

HEK 293 cells

Applications

SDS-PAGE

Accession
Q8R183-1
Animal free

No

Species

Mouse

Reconstitution

Reconstitute vial with 100 µL sterile water. Working aliquots are stable for up to 3 months when stored at -20°C.

Concentration
Loading...
Storage buffer

Constituents: PBS

Sequence info

Amino acid sequence

H T A V Q G L A G H P V T L P C I Y S T H L G G I V P M C W G L G E C R H S Y C I R S L I W T N G Y T V T H Q R N S L Y Q L K G N I S E G N V S L T I E N T V V G D G G P Y C C V V E I P G A F H F V D Y M L E V K P E I S T S P P T R P T A T G R P T T I S T R S T H V P T S T R V S T S T S P T P A H T E T Y K P E A T T F Y P D Q T T A E V T E T L P D T P A D W H N T V T S S D D P W D D N T E V I P P Q K P Q K N L N

Accession

Q8R183

Protein length

Fragment

Predicted molecular weight

65 kDa

Amino acids

22 to 229

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Cell surface glycoprotein that participates in iron homeostasis in the liver, the kidney, the retina and oligodendrocytes by acting as a receptor of H-ferritin (PubMed:16203866, PubMed:33065090). Mechanistically, mediates iron-containing ferritin uptake via an endocytic pathway, trafficking to endosomes and subsequently to lysosomes (PubMed:16043519, PubMed:21886823). Plays also an important role in the regulation of Th2 immunity (PubMed:16043519). Receptor for SEMA4A involved in the regulation of T-cell function, enhancing T-cell activation (PubMed:12374982).

Sequence similarities

Belongs to the immunoglobulin superfamily. TIM family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

T-cell immunoglobulin and mucin domain 2 (Tim-2) also known as HAVCR2 is a protein with a molecular mass of approximately 80 kDa. Tim-2 expresses mainly in the immune system particularly in T cells B cells and dendritic cells. It acts as a receptor with a potential role in regulating the immune response. Tim-2 also presents in various tissues including the liver and kidney suggesting its involvement in multiple physiological processes.

Biological function summary

Tim-2 plays an important role in immune regulation and functions as a part of the broader family of T-cell immunoglobulin and mucin domain proteins. It does not typically form part of a complex but interacts with phosphatidylserine a phospholipid exposed during cell apoptosis. This interaction suggests Tim-2 has roles in recognizing apoptotic cells and may influence their removal by macrophages. Additionally it affects lymphocyte activity possibly by modulating T-cell proliferation and cytokine release.

Pathways

Tim-2 influences immune responses and cell survival pathways. It connects to the phosphatidylserine-mediated clearance pathway a critical component of maintaining tissue homeostasis and preventing inflammation by promoting the clearance of dying cells. Tim-2 also interacts with related proteins such as Tim-3 which shares similar ligand-binding properties and roles in immune response modulation.

Associated diseases and disorders

Tim-2 relates to its role in immune system regulation and its connection to inflammatory disorders and autoimmune diseases. It is implicated in conditions like multiple sclerosis and asthma. In multiple sclerosis Tim-2 and Tim-3 can influence the immune tolerance and inflammation rate affecting disease progression. These connections highlight Tim-2 as a potential target for therapeutic interventions in autoimmune and inflammatory diseases.

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