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AB314516

Recombinant mouse TMC1 protein (His tag)

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Recombinant mouse TMC1 protein (His tag) is a Mouse Full Length protein, in the 1 to 757 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Bth, dn, Tmc1, Transmembrane channel-like protein 1, Beethoven protein, Deafness protein, Transmembrane cochlear-expressed protein 1

1 Images
SDS-PAGE - Recombinant mouse TMC1 protein (His tag) (AB314516)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse TMC1 protein (His tag) (AB314516)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q8R4P5

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in water

Storage buffer

pH: 7.4 - 8 Constituents: 6% Trehalose, 0.87% Ethylenediaminetetraacetic acid, disodium, dihydrate, 0.24% Tris, 0.05% dodecyl 2-(trimethylazaniumyl)ethyl phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC/-80degC. Our default final concentration of glycerol is 50%.
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Sequence info

[{"sequence":"MLQIQVEEKEEDTEESSSEEEEDKLPRRESLRPKRKRTRDVINEDDPEPEPEDEETRKAREKERRRRLRRGAEEEEEIDEEELERLKALLDENRQMIATVKCKPWKMEKKIEVLKEAKKFVSENEGALGKGKGKKWFAFKMMMAKKWAKFLRDFENFKAACVPWENKIKAIESQFGSSVASYFLFLRWMYGVNMVLFVLTFSLIMLPEYLWGLPYGSLPRKTVPRAEEASAANFGVLYDFNGLAQYSVLFYGYYDNKRTIGWLNFRLPLSYFLVGIMCIGYSFLVVLKAMTKNIGDDGGGDDNTFNFSWKVFCSWDYLIGNPETADNKFNSITMNFKEAIIEERAAQVEENIHLIRFLRFLANFFVFLTLGASGYLIFWAVKRSQEFAQQDPDTLGWWEKNEMNMVMSLLGMFCPTLFDLFAELEDYHPLIALKWLLGRIFALLLGNLYVFILALMDEINNKIEEEKLVKANITLWEANMIKAYNESLSGLSGNTTGAPFFVHPADVPRGPCWETMVGQEFVRLTVSDVLTTYVTILIGDFLRACFVRFCNYCWCWDLEYGYPSYTEFDISGNVLALIFNQGMIWMGSFFAPSLPGINILRLHTSMYFQCWAVMCCNVPEARVFKASRSNNFYLGMLLLILFLSTMPVLYMIVSLPPSFDCGPFSGKNRMFEVIGETLEHDFPSWMAKILRQLSNPGLVIAVILVMVLTIYYLNATAKGQKAANLDLKKKMKQQALENKMRNKKMAAARAAAAAGGQ","proteinLength":"Full Length","predictedMolecularWeight":"90.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":757,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8R4P5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C|-80°C
Appropriate long-term storage conditions
-20°C|-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TMC1 also known as Transmembrane Channel-Like 1 is a protein that plays a vital role in mechanotransduction in hair cells of the inner ear. It is a relatively large protein with a mass of approximately 99 kDa. TMC1 is expressed mainly in cochlear and vestibular hair cells where it is believed to form or contribute to the formation of ion channels. These ion channels are essential for converting mechanical stimuli into electrical signals. This mechanistic function is critical for auditory and balance performance.
Biological function summary

TMC1 influences cellular responses to mechanical stimuli in the sensory cells. TMC1 functions as part of a complex with other proteins such as TMIE and LHFPL5 contributing to the transduction complex in hair cells. This arrangement enables the translation of physical forces into changes in membrane potential an essential process for hearing. Any disruption in this mechanism can result in profound hearing impairment due to hair cell dysfunction.

Pathways

TMC1 is central to the mechanotransduction pathway particularly in hair cells. This protein supports the conversion of sound-induced vibrations into neural signals an important process in the auditory pathway. TMC1 closely associates with proteins such as TMIE and LHFPL5 indicating a collective role in ensuring accurate signal transduction. Disturbances in this pathway can undermine the auditory system's ability to interpret external sounds correctly.

TMC1 has significant associations with hearing loss conditions such as DFNA36 a form of autosomal dominant non-syndromic hearing loss and DFNB7/B11 an autosomal recessive hearing impairment. Mutations in TMC1 disrupt its function resulting in defects in the mechanotransduction process in hair cells. This malfunction is often linked to genetic hearing loss where TMC1 mutations lead to deafness through altered interactions with proteins like TMIE and LHFPL5 highlighting the protein's importance in maintaining proper auditory function.
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Specifications

Form

Lyophilized

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General info

Function

Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (PubMed : 11850618, PubMed : 23871232, PubMed : 30138589, PubMed : 36191207). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, the tip-link PCDH15, and possibly the PIEZO subunits (PubMed : 30138589, PubMed : 36191207). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (PubMed : 30138589, PubMed : 36191207). TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (PubMed : 23871232, PubMed : 30138589, PubMed : 36191207). Also involved in vestibular hair cells transduction current (PubMed : 23871232).

Sequence similarities

Belongs to the TMC family.

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Product protocols

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Target data

Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory system (PubMed : 11850618, PubMed : 23871232, PubMed : 30138589, PubMed : 36191207). The MET complex is composed of two dimeric pore-forming ion-conducting transmembrane TMC (TMC1 or TMC2) subunits, several auxiliary proteins including LHFPL5, TMIE, CIB2/3 and TOMT, the tip-link PCDH15, and possibly the PIEZO subunits (PubMed : 30138589, PubMed : 36191207). MET channel is activated by tension in the tip-link extending from the side wall of one stereocilium to the tip of the adjacent shorter stereocilium, where the channel is located (PubMed : 30138589, PubMed : 36191207). TMC1 MET channel is highly permeable to calcium and likely transports monovalent cations (PubMed : 23871232, PubMed : 30138589, PubMed : 36191207). Also involved in vestibular hair cells transduction current (PubMed : 23871232).
See full target information Tmc1
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Complementary Products

Primary Antibodies

AB199949

Anti-TMC1 antibody [EPR19527]

RabMAb|Recombinant

Western blot - Anti-TMC1 antibody [EPR19527] (AB199949)

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