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Recombinant mouse TNF alpha protein (Active) is a Mouse Full Length protein, in the 57 to 235 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

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Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biologically active

Yes

Sequence

G P Q R D E K F P N G L P L I S S M A Q T L T L R S S S Q N S S D K P V A H V V A N H Q V E E Q L E W L S Q R A N A L L A N G M D L K D N Q L V V P A D G L Y L V Y S Q V L F

K G Q G C P D Y V L L T H T V S R F A I S Y Q E K V N L L S A V K S P C P K D T P E G A E L K P W Y E P I Y L G G V F Q L E K G D Q L S A E V N L P K Y L D F A E S G Q V Y F G V I A L

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Cell Culture
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant mouse TNF alpha protein (Active) is a Mouse Full Length protein, in the 57 to 235 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC.

Key facts

Purity

>=95% SDS-PAGE

Endotoxin level

< 0.005 EU/µg

Expression system

HEK 293 cells

Applications

sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC

Biological activity

Fully active compared to a standard. The ED50 as determined by the dose-dependant Killing/apoptosis of L-929 cells is 1.12ng/mL corresponding to a Specific Activity of 8.93 x 105 IU/mg.

Predicted molecular weight

19.8 kDa

Amino acids

57 to 235

Sequence

G P Q R D E K F P N G L P L I S S M A Q T L T L R S S S Q N S S D K P V A H V V A N H Q V E E Q L E W L S Q R A N A L L A N G M D L K D N Q L V V P A D G L Y L V Y S Q V L F

K G Q G C P D Y V L L T H T V S R F A I S Y Q E K V N L L S A V K S P C P K D T P E G A E L K P W Y E P I Y L G G V F Q L E K G D Q L S A E V N L P K Y L D F A E S G Q V Y F G V I A L

Accession
P06804-1
Protein length

Full Length

Animal free

Yes

Nature

Recombinant

Species

Mouse

Reconstitution

Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

Concentration
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Storage buffer

pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Specifications

Form

Lyophilized

Additional notes

Purity by HPLC >=95%.

General info

Function

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity).

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space (By similarity). The soluble form derives from the membrane form by proteolytic processing.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment

Product promise

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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