Recombinant mouse TNF alpha protein (Active) is a Mouse Full Length protein, in the 57 to 235 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC.
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- Acta neuropathologica communications 11:1352023Inhibition of acid sphingomyelinase reduces reactive astrocyte secretion of mitotoxic extracellular vesicles and improves Alzheimer's disease pathology in the 5xFAD mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimone M Crivelli,Zainuddin Quadri,Hemendra J Vekaria,Zhihui Zhu,Priyanka Tripathi,Ahmed Elsherbini,Liping Zhang,Patrick G Sullivan,Erhard BieberichPubMed 37605262
- Science advances 8:eabm25452022Microglial GPR56 is the molecular target of maternal immune activation-induced parvalbumin-positive interneuron deficits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiankun Yu,Tao Li,Jean-Christophe Delpech,Beika Zhu,Priya Kishore,Tatsuhiro Koshi,Rong Luo,Karishma J B Pratt,Galina Popova,Tomasz J Nowakowski,Saul A Villeda,Xianhua PiaoPubMed 35544642
- PLoS pathogens 17:e10099272021Host phospholipid peroxidation fuels ExoU-dependent cell necrosis and supports Pseudomonas aeruginosa-driven pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSalimata Bagayoko,Stephen Adonai Leon-Icaza,Miriam Pinilla,Audrey Hessel,Karin Santoni,David Péricat,Pierre-Jean Bordignon,Flavie Moreau,Elif Eren,Aurélien Boyancé,Emmanuelle Naser,Lise Lefèvre,Céline Berrone,Nino Iakobachvili,Arnaud Metais,Yoann Rombouts,Geanncarlo Lugo-Villarino,Agnès Coste,Ina Attrée,Dara W Frank,Hans Clevers,Peter J Peters,Céline Cougoule,Rémi Planès,Etienne MeunierPubMed 34516571
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
Amino acid sequence
G P Q R D E K F P N G L P L I S S M A Q T L T L R S S S Q N S S D K P V A H V V A N H Q V E E Q L E W L S Q R A N A L L A N G M D L K D N Q L V V P A D G L Y L V Y S Q V L F K G Q G C P D Y V L L T H T V S R F A I S Y Q E K V N L L S A V K S P C P K D T P E G A E L K P W Y E P I Y L G G V F Q L E K G D Q L S A E V N L P K Y L D F A E S G Q V Y F G V I A L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed:32741026). The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Alternative names
Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a
Recommended products
Recombinant mouse TNF alpha protein (Active) is a Mouse Full Length protein, in the 57 to 235 aa range, expressed in HEK 293, with >=95% purity, < 0.005 EU/µg endotoxin level and suitable for sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- < 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- sELISA, SDS-PAGE, FuncS, MS, Cell Culture, HPLC
- Biologically active
- Yes
- Biological activity
- Fully active compared to a standard. The ED50 as determined by the dose-dependant Killing/apoptosis of L-929 cells is 1.12ng/mL corresponding to a Specific Activity of 8.93 x 105 IU/mg.
- Accession
- P06804-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- G P Q R D E K F P N G L P L I S S M A Q T L T L R S S S Q N S S D K P V A H V V A N H Q V E E Q L E W L S Q R A N A L L A N G M D L K D N Q L V V P A D G L Y L V Y S Q V L F K G Q G C P D Y V L L T H T V S R F A I S Y Q E K V N L L S A V K S P C P K D T P E G A E L K P W Y E P I Y L G G V F Q L E K G D Q L S A E V N L P K Y L D F A E S G Q V Y F G V I A L
- Accession
- P06804
- Protein length
- Full Length
- Predicted molecular weight
- 19.8 kDa
- Amino acids
- 57 to 235
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed:25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed:32741026).
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space (By similarity). The soluble form derives from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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Functional Studies - Recombinant mouse TNF alpha protein (Active) (ab259411)
Fully active compared to a standard. The ED50 as determined by the dose-dependant Killing/apoptosis of L-929 cells is 1.12ng/mL corresponding to a Specific Activity of 8.93 x 105 IU/mg.
SDS-PAGE - Recombinant mouse TNF alpha protein (Active) (ab259411)
SDS-PAGE analysis of ab259411.
HPLC - Recombinant mouse TNF alpha protein (Active) (ab259411)
Purity 100%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
Mass Spectrometry - Recombinant mouse TNF alpha protein (Active) (ab259411)
M + 1.5 Da (Calc mass 19797.5 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
Sandwich ELISA - Recombinant mouse TNF alpha protein (Active) (ab259411)
Background subtracted standard curve using Mouse TNF alpha Antibody Pair - BSA and Azide free (Mouse TNF alpha Antibody Pair - BSA and Azide free ab241672) and Recombinant mouse TNF alpha protein (Active) (ab259411) in sandwich ELISA.
The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
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