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AB219721

Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active)

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Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) is a Mouse Fragment protein, in the 47 to 173 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.

View Alternative Names

Gitrl, Tnfsf18, Tumor necrosis factor ligand superfamily member 18, GITR ligand, Glucocorticoid-induced TNF-related ligand, GITRL

3 Images
Functional Studies - Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (AB219721)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (AB219721)

Immobilized Mouse GITR, His Tag at 10 μg/mL (100 μL/well) can bind ab219721 with a linear range of 0.007-0.256 μg/mL.

Functional Studies - Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (AB219721)
  • FuncS

Unknown

Functional Studies - Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (AB219721)

Immobilized Mouse GITR (His Tag) at 5 µg/mL (100 µL/well) can bind Mouse GITR Ligand (Fc Tag) with a linear range of 5-156 ng/mL (QC tested).

SDS-PAGE - Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (AB219721)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse TNFSF18/GITRL protein (Fc Chimera Active) (AB219721)

SDS-PAGE analysis of ab219721 under reducing (lane 1) and non-reducing conditions (lane 2); stained overnight with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Measured by its binding ability in a functional ELISA. Immobilized Mouse GITR, His Tag at 10μg/mL (100 μL/well) can bind ab219721 with a linear range of 0.007-0.256 μg/mL.

Accession

Q7TS55

Animal free

No

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Storage buffer

pH: 7.4 Constituents: 5% Trehalose, 0.87% Sodium chloride, 0.75% Glycine, 0.61% Tris, 0.44% L-Arginine

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>The protein migrates as 45 kDa under reducing condition and 80-100 kDa under non-reducing condition on SDS-PAGE gel.</p>" } } }
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Sequence info

[{"sequence":"TAIESCMVKFELSSSKWHMTSPKPHCVNTTSDGKLKILQSGTYLIYGQVIPVDKKYIKDNAPFVVQIYKKNDVLQTLMNDFQILPIGGVYELHAGDNIYLKFNSKDHIQKTNTYWGIILMPDLPFIS","proteinLength":"Fragment","predictedMolecularWeight":"41.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":173,"aminoAcidStart":47,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q7TS55","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Lyophilized

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General info

Function

Cytokine that binds to TNFRSF18/AITR/GITR (PubMed : 14521928, PubMed : 14647196). Regulates T-cell responses (PubMed : 14647196). Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation (PubMed : 14608036, PubMed : 15128759). Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B (PubMed : 14521928, PubMed : 14647196, PubMed : 18178614). Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (By similarity). Promotes leukocyte adhesion to endothelial cells (PubMed : 23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (PubMed : 24107315).

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Cytokine that binds to TNFRSF18/AITR/GITR (PubMed : 14521928, PubMed : 14647196). Regulates T-cell responses (PubMed : 14647196). Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation (PubMed : 14608036, PubMed : 15128759). Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B (PubMed : 14521928, PubMed : 14647196, PubMed : 18178614). Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1 (By similarity). Promotes leukocyte adhesion to endothelial cells (PubMed : 23892569). Regulates migration of monocytes from the splenic reservoir to sites of inflammation (PubMed : 24107315).
See full target information Tnfsf18
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