Recombinant Mouse Transferrin Receptor 2/TFR2 protein (Fc Chimera) is a Mouse Fragment protein, in the 103 to 798 aa range, expressed in HEK 293, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
R G S C Q A C G D S V L V V D E D V N P E D S G R T T L Y W S D L Q A M F L R F L G E G R M E D T I R L T S L R E R V A G S A R M A T L V Q D I L D K L S R Q K L D H V W T D T H Y V G L Q F P D P A H A N T L H W V D A D G S V Q E Q L P L E D P E V Y C P Y S A T G N A T G K L V Y A H Y G R S E D L Q D L K A K G V E L A G S L L L V R V G I T S F A Q K V A V A Q D F G A Q G V L I Y P D P S D F S Q D P H K P G L S S H Q A V Y G H V H L G T G D P Y T P G F P S F N Q T Q F P P V E S S G L P S I P A Q P I S A D I A D Q L L R K L T G P V A P Q E W K G H L S G S P Y R L G P G P D L R L V V N N H R V S T P I S N I F A C I E G F A E P D H Y V V I G A Q R D A W G P G A A K S A V G T A I L L E L V R T F S S M V S N G F R P R R S L L F I S W D G G D F G S V G A T E W L E G Y L S V L H L K A V V Y V S L D N S V L G D G K F H A K T S P L L V S L I E N I L K Q V D S P N H S G Q T L Y E Q V A L T H P S W D A E V I Q P L P M D S S A Y S F T A F A G V P A V E F S F M E D D R V Y P F L H T K E D T Y E N L H K M L R G R L P A V V Q A V A Q L A G Q L L I R L S H D H L L P L D F G R Y G D V V L R H I G N L N E F S G D L K E R G L T L Q W V Y S A R G D Y I R A A E K L R K E I Y S S E R N D E R L M R M Y N V R I M R V E F Y F L S Q Y V S P A D S P F R H I F L G Q G D H T L G A L V D H L R M L R A D G S G A A S S R L T A G L G F Q E S R F R R Q L A L L T W T L Q G A A N A L S G D V W N I D N N F
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
Alternative names
Trfr2, Tfr2, Transferrin receptor protein 2, TfR2
Recommended products
Recombinant Mouse Transferrin Receptor 2/TFR2 protein (Fc Chimera) is a Mouse Fragment protein, in the 103 to 798 aa range, expressed in HEK 293, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9JKX3-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- Reconstitute at 100 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: Glycine, L-Arginine, Sodium chloride, 5% Trehalose, 0.61% Tris
Sequence info
Amino acid sequence
- R G S C Q A C G D S V L V V D E D V N P E D S G R T T L Y W S D L Q A M F L R F L G E G R M E D T I R L T S L R E R V A G S A R M A T L V Q D I L D K L S R Q K L D H V W T D T H Y V G L Q F P D P A H A N T L H W V D A D G S V Q E Q L P L E D P E V Y C P Y S A T G N A T G K L V Y A H Y G R S E D L Q D L K A K G V E L A G S L L L V R V G I T S F A Q K V A V A Q D F G A Q G V L I Y P D P S D F S Q D P H K P G L S S H Q A V Y G H V H L G T G D P Y T P G F P S F N Q T Q F P P V E S S G L P S I P A Q P I S A D I A D Q L L R K L T G P V A P Q E W K G H L S G S P Y R L G P G P D L R L V V N N H R V S T P I S N I F A C I E G F A E P D H Y V V I G A Q R D A W G P G A A K S A V G T A I L L E L V R T F S S M V S N G F R P R R S L L F I S W D G G D F G S V G A T E W L E G Y L S V L H L K A V V Y V S L D N S V L G D G K F H A K T S P L L V S L I E N I L K Q V D S P N H S G Q T L Y E Q V A L T H P S W D A E V I Q P L P M D S S A Y S F T A F A G V P A V E F S F M E D D R V Y P F L H T K E D T Y E N L H K M L R G R L P A V V Q A V A Q L A G Q L L I R L S H D H L L P L D F G R Y G D V V L R H I G N L N E F S G D L K E R G L T L Q W V Y S A R G D Y I R A A E K L R K E I Y S S E R N D E R L M R M Y N V R I M R V E F Y F L S Q Y V S P A D S P F R H I F L G Q G D H T L G A L V D H L R M L R A D G S G A A S S R L T A G L G F Q E S R F R R Q L A L L T W T L Q G A A N A L S G D V W N I D N N F
- Accession
- Q9JKX3
- Protein length
- Fragment
- Predicted molecular weight
- 101.1 kDa
- Amino acids
- 103 to 798
- Nature
- Recombinant
- Tags
- Fc tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation.
- Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Transferrin Receptor 2.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Transferrin Receptor 2 (TFR2) is a protein that facilitates iron uptake by binding to transferrin which is an iron-carrier protein in the blood. Also known as TFRC2 or CD71L2 it is a transmembrane glycoprotein with a molecular mass around 90 kDa. TFR2 is mainly expressed in the liver and erythroid cell precursors. It plays an important role in maintaining cellular iron homeostasis by mediating the endocytosis of transferrin-bound iron. Its expression can vary depending on the body’s iron levels and cellular type.
- Biological function summary
TFR2 operates as an iron sensor and regulator influencing hepcidin expression a hormone critical for iron homeostasis. TFR2 is not part of any specific complex but interacts with several proteins such as HFE and TFR1 in iron sensing. It helps communicate the body’s iron status to the liver for the modulation of hepcidin levels. This balance ensures the regulation of systemic iron uptake and release which is essential for various physiological processes like erythropoiesis.
- Pathways
TFR2 is involved prominently in the iron homeostasis pathway and the erythropoiesis pathway. In the iron homeostasis pathway it works alongside hepcidin to monitor and regulate iron levels in the body. The role it plays in erythropoiesis relates to its interaction with other iron-related proteins like TFR1 where it contributes to iron delivery necessary for red blood cell production. These pathways illustrate TFR2's importance in not only iron uptake but also the overall iron regulatory processes that sustain body function.
- Associated diseases and disorders
Defects or mutations in TFR2 are connected to hereditary hemochromatosis which causes iron overload in tissues. Aberrant TFR2 function can disrupt the normal regulation of hepcidin leading to this iron accumulation. It also plays a role in anemia of chronic disease due to its influence on erythropoiesis through interactions with HFE and TFR1. These associations highlight the critical importance of TFR2 in both maintaining iron equilibrium and implicating it in iron-related pathologies.
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1 product image
SDS-PAGE - Recombinant Mouse Transferrin Receptor 2/TFR2 protein (Fc Chimera) (ab221026)
SDS-PAGE analysis of reduced ab221026 stained overnight with Coomassie Blue. The protein migrates as 116 kDa in SDS-PAGE due to glycosylation.
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