Recombinant mouse TRAP/CD40L protein (Active) is a Mouse Fragment protein, in the 115 to 260 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, HPLC.
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- Frontiers in microbiology 13:10176962022The role of SIRT1 in the process of infection of RAW 264.7 macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Dong,Ziyang Jiang,Jianhui Zhang,Hanxiao Qin,Jianping Chen,Qiwei ChenPubMed 36466662
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, FuncS, ELISA, HPLC
- Biologically active
Yes
Amino acid sequence
G D E D P Q I A A H V V S E A N S N A A S V L Q W A K K G Y Y T M K S N L V M L E N G K Q L T V K R E G L Y Y V Y T Q V T F C S N R E P S S Q R P F I V G L W L K P S S G S E R I L L K A A N T H S S S Q L C E Q Q S V H L G G V F E L Q A G A S V F V N V T E A S Q V I H R V G F S S F G L L K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165).CD40 ligand, soluble formActs as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Alternative names
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5, TRAP, Tnfsf5, Cd40lg, Cd40l
Recommended products
Recombinant mouse TRAP/CD40L protein (Active) is a Mouse Fragment protein, in the 115 to 260 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA, HPLC.
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, FuncS, ELISA, HPLC
- Biologically active
Yes
- Biological activity
Measured by its binding ability in a functional ELISA against immobilized Mouse CD40, Fc Tag (0.2 μg/well). The linear range is 9-78 ng/ml.
- Accession
- P27548-1
- Animal free
No
- Species
Mouse
- Reconstitution
Reconstitute at 200 µg/mL in water
- Concentration
- Storage buffer
pH: 7.4
Constituents: 95% PBS, 5% Trehalose
Sequence info
Amino acid sequence
G D E D P Q I A A H V V S E A N S N A A S V L Q W A K K G Y Y T M K S N L V M L E N G K Q L T V K R E G L Y Y V Y T Q V T F C S N R E P S S Q R P F I V G L W L K P S S G S E R I L L K A A N T H S S S Q L C E Q Q S V H L G G V F E L Q A G A S V F V N V T E A S Q V I H R V G F S S F G L L K L
- Accession
- Protein length
Fragment
- Predicted molecular weight
16.8 kDa
- Amino acids
115 to 260
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Lyophilized
- Additional notes
>95% as determined by SDS-PAGE.>95% as determined by SEC-MALS.
General info
- Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165).
- Sequence similarities
Belongs to the tumor necrosis factor family.
- Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRAP/CD40L also known as CD154 or CD40 ligand is a type II transmembrane protein with a molecular mass of approximately 39 kDa. CD40L is mainly expressed on the surface of activated T cells. This protein binds to CD40 a significant receptor present on several immune cells including B cells macrophages and dendritic cells. The interaction with CD40 is essential for immune cell activation and development marking CD40L as an important player in adaptive immunity.
- Biological function summary
The interaction between CD40L and CD40 triggers cell proliferation differentiation and apoptosis in immune cells. CD40L contributes to the formation of an immunological synapse encompassing its role in T helper cell function. Furthermore CD40L is implicated in the activation of B cells which are necessary for antibody class switching and memory cell generation. Its broad influence shows CD40L's involvement in immune system regulation and response.
- Pathways
CD40L is integral to the CD40 signaling pathway which is involved in immune and inflammatory responses. CD40L activates the NF-kB and JNK signaling pathways by engaging with CD40 which then drives the transcription of genes related to immune response. This signaling cascade impacts several other proteins such as TRAF proteins which act as intermediaries in propagating the signal.
- Associated diseases and disorders
CD40L has been linked to immune-related conditions including autoimmune disorders and inflammatory diseases. Elevated CD40L expression is observed in diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. In SLE CD40L interaction with CD40 on B cells is known to exacerbate disease severity by promoting autoantibody production. Exploring anti-CD40L therapeutic strategies can offer potential intervention in such diseases.
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3 product images
SDS-PAGE - Recombinant mouse TRAP/CD40L protein (Active) (ab220551)
SDS-PAGE analysis of ab220551 stained overnight with Coomassie Blue.
The reduced protein migrates as 21-25 kDa in SDS-PAGE.
Functional Studies - Recombinant mouse TRAP/CD40L protein (Active) (ab220551)
Immobilized Mouse CD40, Fc Tag at 2 μg/ml (100 μl/well) can bind ab220551 with a linear range of 9-78 ng/ml.
HPLC - Recombinant mouse TRAP/CD40L protein (Active) (ab220551)
A SEC-HPLC analysis showing over 95% of ab220551 present as active trimers with a molecular mass of around 50-65 kDa.
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