JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224868

Recombinant Mouse TRP2/DCT protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Mouse TRP2/DCT protein (His tag) is a Mouse Fragment protein, in the 24 to 472 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Tyrp-2, Tyrp2, Dct, L-dopachrome tautomerase, DCT, DT, DOPAchrome conversion factor, DOPAchrome isomerase, DOPAchrome oxidoreductase, L-dopachrome Delta-isomerase, SLATY locus protein, Tyrosinase-related protein 2, TRP-2, TRP2

1 Images
SDS-PAGE - Recombinant Mouse TRP2/DCT protein (His tag) (AB224868)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse TRP2/DCT protein (His tag) (AB224868)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P29812

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Previously labelled as TRP2
style
left-column

Sequence info

[{"sequence":"QFPRVCMTLDGVLNKECCPPLGPEATNICGFLEGRGQCAEVQTDTRPWSGPYILRNQDDREQWPRKFFNRTCKCTGNFAGYNCGGCKFGWTGPDCNRKKPAILRRNIHSLTAQEREQFLGALDLAKKSIHPDYVITTQHWLGLLGPNGTQPQIANCSVYDFFVWLHYYSVRDTLLGPGRPYKAIDFSHQGPAFVTWHRYHLLWLERELQRLTGNESFALPYWNFATGKNECDVCTDELLGAARQDDPTLISRNSRFSTWEIVCDSLDDYNRRVTLCNGTYEGLLRRNKVGRNNEKLPTLKNVQDCLSLQKFDSPPFFQNSTFSFRNALEGFDKADGTLDSQVMNLHNLAHSFLNGTNALPHSAANDPVFVVLHSFTDAIFDEWLKRNNPSTDAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTAEQLGYNYAVDLSEEEAPVWSTTLS","proteinLength":"Fragment","predictedMolecularWeight":"69.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":472,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P29812","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRP2 also known as DCT or dopachrome tautomerase is a protein involved in melanin synthesis and is part of the tyrosinase-related protein family. It has a molecular weight of approximately 60 kDa. TRP2/DCT is mainly expressed in melanocytes which are cells responsible for pigment production. The protein functions in the melanin biosynthetic pathway converting dopachrome into DHICA contributing to the eumelanin formation which impacts skin and hair color.
Biological function summary

TRP2/DCT participates in pigmentation and photoprotection processes. It functions in tandem with other enzymes like tyrosinase and TRP1 to form a multiprotein complex important for melanin synthesis. The coordination of these proteins ensures the effective production and regulation of melanin which absorbs ultraviolet radiation and reduces DNA damage in skin cells.

Pathways

TRP2/DCT plays a role in the melanin biosynthetic pathway and influences the Wnt signaling pathway. The melanin pathway involves enzymes such as tyrosinase which initiates the conversion of tyrosine to L-DOPA and eventually to melanin pigments. The Wnt signaling pathway critical for cellular processes like proliferation and differentiation interacts with melanogenesis through proteins like beta-catenin influencing TRP2/DCT expression and activity.

TRP2/DCT has significant associations with melanoma and vitiligo. In melanoma altered expression of TRP2/DCT can impact tumor cell proliferation and survival often interacting with proteins like MITF an important regulator of melanocyte development. In vitiligo changes in TRP2/DCT expression can disrupt melanin production leading to depigmented patches on the skin. Understanding these interactions and expressions aids in potential therapeutic approaches for these skin conditions.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Plays a role in melanin biosynthesis (PubMed : 33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA) (PubMed : 1537333, PubMed : 1537334).

Sequence similarities

Belongs to the tyrosinase family.

Post-translational modifications

Glycosylated.

style
left-column

Product protocols

style
left-column

Target data

Plays a role in melanin biosynthesis (PubMed : 33100333). Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA) (PubMed : 1537333, PubMed : 1537334).
See full target information Dct
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB132146

Recombinant Human Melanoma gp100 protein

SDS-PAGE - Recombinant Human Melanoma gp100 protein (AB132146)

  • 0
  • 0
Primary Antibodies

AB221144

Anti-TRP2/DCT antibody [EPR21986]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP2/DCT antibody [EPR21986] (AB221144)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB210546

Anti-MelanA antibody [EPR20380]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [EPR20380] (AB210546)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com