JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB92641

Recombinant mouse Urokinase protein

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant mouse Urokinase protein is a Mouse Full Length protein, in the 179 to 433 aa range, expressed in Insect cells, with >98%, suitable for SDS-PAGE, ELISA, FuncS.

View Alternative Names

Urokinase-type plasminogen activator, U-plasminogen activator, uPA, Plau

Key facts

Purity

>98% SDS-PAGE

Expression system

Insect cells

Tags

Tag free

Applications

FuncS, SDS-PAGE, ELISA

applications

Biologically active

Yes

Biological activity

The uPA activity of this product was tested using urokinase chromogenic substrate and standardized to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. If you need the specific activity value please contact us with the lot# you require the data for.

Accession

P06869

Animal free

No

Carrier free

No

Species

Mouse

Storage buffer

pH: 5 Constituents: 0.58% Sodium chloride, 0.41% Sodium acetate, 0.0292% EDTA

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Spectrophotometric Data:

Ultraviolet Absorbance (280nm) = 0.9

epsilon 0.1% = 1.48

ab92641 demonstrates preferential binding to mouse uPAR over Human uPAR coated on an ELISA plate.

Produced using non-baculovirus insect cells.

style
left-column

Sequence info

[{"sequence":"KIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF","proteinLength":"Full Length","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":433,"aminoAcidStart":179,"nature":"Recombinant","expressionSystem":"Insect cells","accessionNumber":"P06869","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.
Biological function summary

Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.

Pathways

Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.

Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.

Sequence similarities

Belongs to the peptidase S1 family.

Post-translational modifications

Produced as an inactive single-chain protein (pro-uPA or sc-uPA), is processed into the active disulfide-linked two-chain form of PLAU/uPA by a proteolytic event mediated, at least, by TMPRSS4.

style
left-column

Product protocols

style
left-column

Target data

Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
See full target information Urokinase-type plasminogen activator
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 235:6268-6286 PubMed31990070

2020

Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina V Semina,Kseniya A Rubina,Anna A Shmakova,Karina D Rysenkova,Polina S Klimovich,Natalya A Aleksanrushkina,Veronika Y Sysoeva,Maxim N Karagyaur,Vsevolod A Tkachuk
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB90928

Native human Plasmin protein (Active)

SDS-PAGE - Native human Plasmin protein (Active) (AB90928)

  • 0
  • 0
Primary Antibodies

AB66705

Anti-PAI1 antibody

BOND RX™ Validated

Western blot - Anti-PAI1 antibody (AB66705)

  • 4
  • 8
Primary Antibodies

AB16663

Anti-Cyclin D1 antibody [SP4]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [SP4] (AB16663)

  • 4
  • 23

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com