Recombinant Mouse VCAM1 Protein (Fc Chimera) is a Mouse Fragment protein, in the 25 to 698 aa range, expressed in HEK 293, with >=95% purity, > 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- > 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
Amino acid sequence
F K I E I S P E Y K T I A Q I G D S M A L T C S T T G C E S P L F S W R T Q I D S P L N A K V R T E G S K S V L T M E P V S F E N E H S Y L C T A T C G S G K L E R S I H V D I Y S F P K D P E I Q F S G P L E V G K P V T V K C L A P D I Y P V Y R L E I D L F K G D Q L M N R Q E F S S E E M T K S L E T K S L E V T F T P V I E D I G K A L V C R A K L H I D Q I D S T L K E R E T V K E L Q V Y I S P R N T T I S V H P S T R L Q E G G A V T M T C S S E G L P A P E I F W G R K L D N E V L Q L L S G N A T L T L I A M R M E D S G V Y V C E G V N L I G R D K A E V E L V V Q E K P F I V D I S P G S Q V A A Q V G D S V V L T C A A I G C D S P S F S W R T Q T D S P L N G V V R N E G A K S T L V L S S V G F E D E H S Y L C A V T C L Q R T L E K R T Q V E V Y S F P E D P V I K M S G P L V H G R P V T V N C T V P N V Y P F D H L E I E L L K G E T T L M K K Y F L E E M G I K S L E T K I L E T T F I P T I E D T G K S L V C L A R L H S G E M E S E P K Q R Q S V Q P L Y V N V A P K E T T I W V S P S P I L E E G S P V N L T C S S D G I P A P K I L W S R Q L N N G E L Q P L S E N T T L T F M S T K R D D S G I Y V C E G I N E A G I S R K S V E L I I Q V S P K D I Q L T V F P S K S V K E G D T V I I S C T C G N V P E T W I I L K K K A K T G D M V L K S V D G S Y T I R Q A Q L Q D A G I Y E C E S K T E V G S Q L R S L T L D V K G K E H N K D Y F S P E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
Alternative names
CD106, Vcam-1, Vcam1, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1
Recommended products
Recombinant Mouse VCAM1 Protein (Fc Chimera) is a Mouse Fragment protein, in the 25 to 698 aa range, expressed in HEK 293, with >=95% purity, > 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- > 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Accession
- P29533-1
- Animal free
- Yes
- Species
- Mouse
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- F K I E I S P E Y K T I A Q I G D S M A L T C S T T G C E S P L F S W R T Q I D S P L N A K V R T E G S K S V L T M E P V S F E N E H S Y L C T A T C G S G K L E R S I H V D I Y S F P K D P E I Q F S G P L E V G K P V T V K C L A P D I Y P V Y R L E I D L F K G D Q L M N R Q E F S S E E M T K S L E T K S L E V T F T P V I E D I G K A L V C R A K L H I D Q I D S T L K E R E T V K E L Q V Y I S P R N T T I S V H P S T R L Q E G G A V T M T C S S E G L P A P E I F W G R K L D N E V L Q L L S G N A T L T L I A M R M E D S G V Y V C E G V N L I G R D K A E V E L V V Q E K P F I V D I S P G S Q V A A Q V G D S V V L T C A A I G C D S P S F S W R T Q T D S P L N G V V R N E G A K S T L V L S S V G F E D E H S Y L C A V T C L Q R T L E K R T Q V E V Y S F P E D P V I K M S G P L V H G R P V T V N C T V P N V Y P F D H L E I E L L K G E T T L M K K Y F L E E M G I K S L E T K I L E T T F I P T I E D T G K S L V C L A R L H S G E M E S E P K Q R Q S V Q P L Y V N V A P K E T T I W V S P S P I L E E G S P V N L T C S S D G I P A P K I L W S R Q L N N G E L Q P L S E N T T L T F M S T K R D D S G I Y V C E G I N E A G I S R K S V E L I I Q V S P K D I Q L T V F P S K S V K E G D T V I I S C T C G N V P E T W I I L K K K A K T G D M V L K S V D G S Y T I R Q A Q L Q D A G I Y E C E S K T E V G S Q L R S L T L D V K G K E H N K D Y F S P E
- Accession
- P29533
- Protein length
- Fragment
- Predicted molecular weight
- 100.3 kDa
- Actual molecular weight
- 100.3 kDa
- Amino acids
- 25 to 698
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
- Post-translational modifications
Cleaved by the metalloproteinase ADAM17 to generate the soluble form.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
- Biological function summary
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
- Pathways
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
- Associated diseases and disorders
Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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2 product images
SDS-PAGE - Recombinant Mouse VCAM1 Protein (Fc Chimera) (ab310806)
SDS-PAGE analysis of ab310806
Mass Spectrometry - Recombinant Mouse VCAM1 Protein (Fc Chimera) (ab310806)
Mass determination by ESI-TOF. Predicted MW is 100332.36308 (+/- 10Da by ESI-TOF). Observed MW is 100335.73.
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