Recombinant Mouse VCAM1 protein (Fc Chimera His Tag) is a Fragment protein, in the 1 to 698 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M P V K M V A V L G A S T V L W I L F A V S Q A F K I E I S P E Y K T I A Q I G D S M A L T C S T T G C E S P L F S W R T Q I D S P L N A K V R T E G S K S V L T M E P V S F E N E H S Y L C T A T C G S G K L E R S I H V D I Y S F P K D P E I Q F S G P L E V G K P V T V K C L A P D I Y P V Y R L E I D L F K G D Q L M N R Q E F S S E E M T K S L E T K S L E V T F T P V I E D I G K A L V C R A K L H I D Q I D S T L K E R E T V K E L Q V Y I S P R N T T I S V H P S T R L Q E G G A V T M T C S S E G L P A P E I F W G R K L D N E V L Q L L S G N A T L T L I A M R M E D S G V Y V C E G V N L I G R D K A E V E L V V Q E K P F I V D I S P G S Q V A A Q V G D S V V L T C A A I G C D S P S F S W R T Q T D S P L N G V V R N E G A K S T L V L S S V G F E D E H S Y L C A V T C L Q R T L E K R T Q V E V Y S F P E D P V I K M S G P L V H G R P V T V N C T V P N V Y P F D H L E I E L L K G E T T L M K K Y F L E E M G I K S L E T K I L E T T F I P T I E D T G K S L V C L A R L H S G E M E S E P K Q R Q S V Q P L Y V N V A P K E T T I W V S P S P I L E E G S P V N L T C S S D G I P A P K I L W S R Q L N N G E L Q P L S E N T T L T F M S T K R D D S G I Y V C E G I N E A G I S R K S V E L I I Q V S P K D I Q L T V F P S K S V K E G D T V I I S C T C G N V P E T W I I L K K K A K T G D M V L K S V D G S Y T I R Q A Q L Q D A G I Y E C E S K T E V G S Q L R S L T L D V K G K E H N K N Y F S P E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Recommended products
Recombinant Mouse VCAM1 protein (Fc Chimera His Tag) is a Fragment protein, in the 1 to 698 aa range, expressed in HEK 293, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Fc tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Animal free
- No
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
- M P V K M V A V L G A S T V L W I L F A V S Q A F K I E I S P E Y K T I A Q I G D S M A L T C S T T G C E S P L F S W R T Q I D S P L N A K V R T E G S K S V L T M E P V S F E N E H S Y L C T A T C G S G K L E R S I H V D I Y S F P K D P E I Q F S G P L E V G K P V T V K C L A P D I Y P V Y R L E I D L F K G D Q L M N R Q E F S S E E M T K S L E T K S L E V T F T P V I E D I G K A L V C R A K L H I D Q I D S T L K E R E T V K E L Q V Y I S P R N T T I S V H P S T R L Q E G G A V T M T C S S E G L P A P E I F W G R K L D N E V L Q L L S G N A T L T L I A M R M E D S G V Y V C E G V N L I G R D K A E V E L V V Q E K P F I V D I S P G S Q V A A Q V G D S V V L T C A A I G C D S P S F S W R T Q T D S P L N G V V R N E G A K S T L V L S S V G F E D E H S Y L C A V T C L Q R T L E K R T Q V E V Y S F P E D P V I K M S G P L V H G R P V T V N C T V P N V Y P F D H L E I E L L K G E T T L M K K Y F L E E M G I K S L E T K I L E T T F I P T I E D T G K S L V C L A R L H S G E M E S E P K Q R Q S V Q P L Y V N V A P K E T T I W V S P S P I L E E G S P V N L T C S S D G I P A P K I L W S R Q L N N G E L Q P L S E N T T L T F M S T K R D D S G I Y V C E G I N E A G I S R K S V E L I I Q V S P K D I Q L T V F P S K S V K E G D T V I I S C T C G N V P E T W I I L K K K A K T G D M V L K S V D G S Y T I R Q A Q L Q D A G I Y E C E S K T E V G S Q L R S L T L D V K G K E H N K N Y F S P E
- Protein length
- Fragment
- Predicted molecular weight
- 102 kDa
- Amino acids
- 1 to 698
- Nature
- Recombinant
- Tags
- Fc tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Lyophilized
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
- Biological function summary
Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.
- Pathways
VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.
- Associated diseases and disorders
Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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SDS-PAGE - Recombinant Mouse VCAM1 protein (Fc Chimera His Tag) (ab276780)
SDS-PAGE analysis of ab276780
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