Recombinant mouse VEGFA protein (Active)

• FuncS

Unknown

Functional Studies - Recombinant mouse VEGFA protein (Active) (AB185265)

The purity of ActiveMax® Mouse VEGF164 was greater than 95% as determined by SEC-HPLC.

• FuncS

Unknown

Functional Studies - Recombinant mouse VEGFA protein (Active) (AB185265)

Immobilized ab185265 at 2 μg/mL (100 μL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL.

• WB

Lab

Western blot - Recombinant mouse VEGFA protein (Active) (AB185265)

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab46154 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-VEGFA antibody (<a href='/en-us/products/primary-antibodies/vegfa-antibody-ab46154'>ab46154</a>) at 1 µg/mL

Lane 1:

Recombinant Human VEGFA protein (His tag) (<a href='/en-us/products/unavailable/recombinant-human-vegfa-protein-his-tag-ab204773'>ab204773</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant mouse VEGFA protein (Active) (ab185265) at 0.1 µg

Secondary

All lanes:

Rabbit IgG secondary antibody at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 23 kDa

true

Exposure time: 10s

• SDS-PAGE

Unknown

SDS-PAGE - Recombinant mouse VEGFA protein (Active) (AB185265)

ab185265 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.