Recombinant Mouse VISTA protein is a Mouse Fragment protein, in the 33 to 191 aa range, expressed in Insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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- Biochemical and biophysical research communication 516:914-9202019Inhibition of allogeneic islet graft rejection by VISTA-conjugated liposome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Guo et. al.PubMed 31272717
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
F K V T T P Y S L Y V C P E G Q N A T L T C R I L G P V S K G H D V T I Y K T W Y L S S R G E V Q M C K E H R P I R N F T L Q H L Q H H G S H L K A N A S H D Q P Q K H G L E L A S D H H G N F S I T L R N V T P R D S G L Y C C L V I E L K N H H P E Q R F Y G S M E L Q V Q A G K G S G S T C M A S N E Q D S D S I T A A L E H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).
Alternative names
Dies1, PD-1H, VISTA, Vsir, V-type immunoglobulin domain-containing suppressor of T-cell activation, Platelet receptor Gi24, V-set domain-containing immunoregulatory receptor, V-set immunoregulatory receptor
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Recombinant Mouse VISTA protein is a Mouse Fragment protein, in the 33 to 191 aa range, expressed in Insect, with >90% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Insect cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q9D659-1
- Animal free
- No
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- F K V T T P Y S L Y V C P E G Q N A T L T C R I L G P V S K G H D V T I Y K T W Y L S S R G E V Q M C K E H R P I R N F T L Q H L Q H H G S H L K A N A S H D Q P Q K H G L E L A S D H H G N F S I T L R N V T P R D S G L Y C C L V I E L K N H H P E Q R F Y G S M E L Q V Q A G K G S G S T C M A S N E Q D S D S I T A A L E H H H H H H
- Accession
- Q9D659
- Protein length
- Fragment
- Predicted molecular weight
- 18.8 kDa
- Amino acids
- 33 to 191
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Immunoregulatory receptor which inhibits the T-cell response (PubMed:21383057, PubMed:24743150, PubMed:25267631). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (PubMed:20042595). May stimulate MMP14-mediated MMP2 activation (By similarity).
- Post-translational modifications
At the cell surface, may be cleaved by MMP14.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
VISTA also known as V-domain Ig suppressor of T cell activation or Gi24 is a protein significant in immune regulation. It is a type I transmembrane glycoprotein with an apparent mass of approximately 55 kDa. VISTA is expressed on hematopoietic cells specifically within immune system components such as myeloid cells and T cells. Researchers have identified biotinylated chimeric proteins which facilitate the study of VISTA's expression and interactions in various immunological research models.
- Biological function summary
VISTA functions in immune checkpoint regulation by modulating T cell activity. It acts as both a ligand and a receptor influencing immune responses. As part of an immune complex VISTA interacts with other checkpoint proteins to maintain immune homeostasis and prevent autoimmunity. Its role mirrors those of other B7 family members like PD-1 and CTLA-4 which are involved in negative immune regulation.
- Pathways
VISTA integrates into the broader framework of immune signaling pathways. It contributes significantly to the immune checkpoint pathway similar to CTLA-4 and PD-1 which mediate immune cell proliferation and activation. By interacting with these proteins VISTA plays an important role in maintaining self-tolerance and regulating inflammatory responses functioning parallel to other proteins such as CD28 and B7-H5 in the immune checkpoint pathway.
- Associated diseases and disorders
VISTA's improper regulation associates with autoimmune diseases and cancer. Its expression influences the progression of conditions like rheumatoid arthritis where it modulates immune responses and inflammation. Additionally in oncology VISTA acts as an inhibitory molecule; hence targeting it can enhance anti-tumor immunity similar to therapies targeting proteins like PD-1 and PD-L1. Understanding VISTA's intricate connections helps in developing therapies for these diseases.
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1 product image
SDS-PAGE - Recombinant Mouse VISTA protein (ab208315)
15% SDS-PAGE analysis of ab208315 (3μg).
Observed MW: 28-40KDa (SDS-PAGE under reducing conditions).
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