AB208465

Recombinant Mouse WIF1 protein (His tag)

Name: Recombinant Mouse WIF1 protein (His tag) (ab208465) | Abcam
Brand: Abcam Limited
SKU: AB208465

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(1 Publication)

Recombinant Mouse WIF1 protein (His tag) is a Mouse Full Length protein, in the 29 to 379 aa range, expressed in Insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Wnt inhibitory factor 1, WIF-1, Wif1

1 Images

SDS-PAGE - Recombinant Mouse WIF1 protein (His tag) (AB208465)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Mouse WIF1 protein (His tag) (AB208465)

15% SDS-PAGE analysis of ab208465 (3 μg)

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Insect cells

Applications

SDS-PAGE

applications

Biologically active

Accession

Q9WUA1

Animal free

Carrier free

Species

Mouse

Storage buffer

pH: 5.5 Constituents: 30% Glycerol (glycerin, glycerine), 0.39% MES, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Sequence info

[{"sequence":"GQPPEESLYLWIDAHQARVLIGFEEDILIVSEGKMAPFTHDFRKAQQRMPAIPVNIHSMNFTWQAAGQAEYFYEFLSLRSLDKGIMADPTVNVPLLGTVPHKASVVQVGFPCLGKQDGVAAFEVNVIVMNSEGNTILRTPQNAIFFKTCQQAECPGGCRNGGFCNERRVCECPDGFYGPHCEKALCIPRCMNGGLCVTPGFCICPPGFYGVNCDKANCSTTCFNGGTCFYPGKCICPPGLEGEQCELSKCPQPCRNGGKCIGKSKCKCPKGYQGDLCSKPVCEPGCGAHGTCHEPNKCQCREGWHGRHCNKRYGASLMHAPRPAGAGLERHTPSLKKAEDRRDPPESNYIWVEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"39.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":379,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Insect cells","accessionNumber":"Q9WUA1","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WIF1 also known as Wnt inhibitory factor 1 encodes a protein that interacts with Wnt proteins and inhibits their activity. This protein has an approximate molecular mass of 40 kDa and is secreted into the extracellular space. It shows expression in various tissues including the brain lung and kidney. The structure of WIF1 enables it to bind directly to Wnt proteins modulating their signaling capabilities.

Biological function summary

WIF1 participates in the regulation of Wnt signaling which is a critical pathway for cell development and differentiation. It functions to maintain cellular homeostasis by preventing excessive Wnt signaling an essential process in embryonic development and adult tissue maintenance. WIF1 does not form a complex but interacts with Wnt proteins in the extracellular environment ensuring that Wnt signaling remains controlled.

Pathways

WIF1 plays a significant role in the Wnt signaling pathway which is essential for processes like cell fate determination and proliferation. By inhibiting Wnt activity WIF1 supports maintaining balanced signaling levels within the pathway. It connects to other components like β-catenin and can influence pathways like the Wnt/β-catenin signaling pathway which involves the accumulation and transcriptional activity of β-catenin in the cell nucleus.

WIF1 has been implicated in cancer and osteoarthritis due to its regulatory effect on Wnt signaling. Downregulation of WIF1 often associates with increased Wnt activity contributing to tumor progression in various cancers through interactions with proteins like DKK1 a known Wnt pathway inhibitor. In osteoarthritis altered WIF1 expression levels can affect cartilage degradation by modifying Wnt signaling showing its role in maintaining joint health.

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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation.

See full target information Wif1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 22:709-718 PubMed30988524

2019

Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqin Niu,Hui-Hsin Tsai,Kimberly K Hoi,Nanxin Huang,Guangdan Yu,Kicheol Kim,Sergio E Baranzini,Lan Xiao,Jonah R Chan,Stephen P J Fancy

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Recombinant Mouse WIF1 protein (His tag)

SDS-PAGE - Recombinant Mouse WIF1 protein (His tag) (AB208465)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Product protocols

Target data

Publications (1)

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