AB81484

Recombinant mouse Wnt3a protein

Name: Recombinant mouse Wnt3a protein (ab81484) | Abcam
Brand: Abcam Limited
SKU: AB81484
Rating: 5 (2 reviews)

(2 Reviews)

(17 Publications)

Recombinant mouse Wnt3a protein is a Mouse Full Length protein, in the 19 to 352 aa range with >75% purity, < 1 EU/µg endotoxin level and suitable for cell differentiation assays, HPLC, and SDS-PAGE. The predicted molecular weight of ab81484 protein is 37.4 kDa.

- Save time and ensure accurate results- use our recombiant Wnt3a protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Cited in over 15 publications

View Alternative Names

Wnt-3a, Wnt3a, Protein Wnt-3a

1 Images

Cell Differentiation - Recombinant mouse Wnt3a protein (AB81484)

CellDiff

Unknown

Cell Differentiation - Recombinant mouse Wnt3a protein (AB81484)

Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification : 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.

This image is courtesy of Emily Moorefield, Wake Forest Institute for Regenerative Medicine, NC, USA

Key facts

Purity

>75% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Cell Culture

Applications

CellAct, SDS-PAGE, FuncS, HPLC, CellDiff

applications

Biologically active

Yes

Biological activity

The ED₅₀ was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.

The expected ED₅₀ for this effect is 0.4-0.6 ng/ml.

Accession

P27467

Animal free

Carrier free

Species

Mouse

Reconstitution

reconstitute with water at 0.1mg/mL

Storage buffer

Constituents: 13% 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "CellDiff": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Due to glycosylation, ab81484 migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non-reducing conditions." }, "CellAct": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"The expected ED50 for the induction of alkaline phosphatase production by CCL-226 cells is 0.4-0.6 ng/ml." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Product details

Ensure the validity of your results using our Recombinant mouse Wnt3a protein ab81484 as a positive control in SDS-PAGE.
Our bioactive recombinant mouse Wnt3 protein ab81484 is ideal to use in oncogenesis research and several developmental processes, including regulation of cell fate and patterning during embryogenesis.

The ab81484 mouse Wnt-3a is a monomeric glycoprotein. Due to glycosylation ab81484 migrates at an observed molecular weight of ~38-41 kDa by SDS-PAGE under non-reducing conditions.

Check out our protein gel staining guide for SDS-PAGE here

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Sequence info

[{"sequence":"SYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":352,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Cell Culture","accessionNumber":"P27467","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

A few weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

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Specifications

Form

Lyophilized

Additional notes

Greater than 75% by HPLC analyses.

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General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites (PubMed : 8299937). Required for normal morphogenesis of the developing neural tube (PubMed : 8299937). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A.. Disulfide bonds have critical and distinct roles in secretion and activity. Loss of each conserved cysteine in WNT3A results in high molecular weight oxidized Wnt oligomers, which are formed through inter-Wnt disulfide bonding.. Palmitoleoylation by PORCN is required for efficient binding to frizzled receptors. Palmitoleoylation is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoleoylated WNT3A from WLS in secretory vesicles (PubMed:17141155, PubMed:24798332). Depalmitoleoylated by NOTUM, leading to inhibit Wnt signaling pathway, possibly by promoting disulfide bond formation and oligomerization (PubMed:25771893).

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Wnt3a

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109247 PubMed38439969

2024

Discovery of non-genomic drivers of YAP signaling modulating the cell plasticity in CRC tumor lines.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuhiko Ogasawara,Yoshihito Kano,Yosuke Yoneyama,Sakurako Kobayashi,Satoshi Watanabe,Sakura Kirino,Fausto D Velez-Bravo,Yourae Hong,Aleksandra Ostapiuk,Pavlo Lutsik,Iichiroh Onishi,Shinichi Yamauchi,Yui Hiraguri,Go Ito,Yusuke Kinugasa,Kenichi Ohashi,Mamoru Watanabe,Ryuichi Okamoto,Sabine Tejpar,Shiro Yui

Current research in neurobiology 5:100095 PubMed37426743

2023

Protocol for a Wnt reporter assay to measure its activity in human neural stem cells derived from induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cristine Marie Yde Ohki,Natalie Monet Walter,Michelle Rickli,José Maria Salazar Campos,Anna Maria Werling,Christian Döring,Susanne Walitza,Edna Grünblatt

Methods in molecular biology (Clifton, N.J.) 2520:295-307 PubMed34611822

2021

Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.

Applications

Unspecified application

Species

Unspecified reactive species

Emre Balbasi,Dersu Sezginmert,Ceren Alganatay,Nihal Terzi Cizmecioglu

Journal of cellular and molecular medicine 25:6963-6975 PubMed34101346

2021

Fractalkine aggravates LPS-induced macrophage activation and acute kidney injury via Wnt/β-catenin signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Gong,Yan Jiang,Xiuhong Pan,Yanwu You

Angiogenesis 24:631-645 PubMed33650028

2021

Loss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Liu,Han Zhou,Tiening Zhang,Xixiang Gao,Bo Tao,Hao Xing,Zhenwu Zhuang,Alan Dardik,Themis R Kyriakides,Julie E Goodwin

American journal of physiology. Gastrointestinal and liver physiology 320:G627-G643 PubMed33566751

2021

Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, βPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kunrong Cheng,Shannon M Larabee,Mazen Tolaymat,Marie Hanscom,Aaron C Shang,Alyssa Schledwitz,Shien Hu,Cinthia B Drachenberg,Min Zhan,Ahmed Chahdi,Jean-Pierre Raufman

Molecular cancer 19:118 PubMed32727463

2020

LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes.

Applications

Unspecified application

Species

Unspecified reactive species

Cefan Zhou,Changhua Yi,Yongxiang Yi,Wenying Qin,Yanan Yan,Xueying Dong,Xuewen Zhang,Yuan Huang,Rui Zhang,Jie Wei,Declan William Ali,Marek Michalak,Xing-Zhen Chen,Jingfeng Tang

International journal of cell biology 2020:2045969 PubMed32328102

2020

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation .

Applications

Unspecified application

Species

Unspecified reactive species

Anisha Polley,Riffat Khanam,Arunima Sengupta,Santanu Chakraborty

Journal of immunology (Baltimore, Md. : 1950) 203:2630-2643 PubMed31586036

2019

Controls Germinal Center Reaction by Relaying a Wnt Signal to the Expression in Centroblasts during Humoral Immune Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Young Choi,Joo Hyun Pi,Sung-Kyun Park,Chang Joong Kang

Science translational medicine 11: PubMed31316008

2019

Calpain 9 as a therapeutic target in TGFβ-induced mesenchymal transition and fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

David H Kim,James D Beckett,Varun Nagpal,Manuel A Seman-Senderos,Russell A Gould,Tyler J Creamer,Elena Gallo MacFarlane,Yichun Chen,Djahida Bedja,Jonathan T Butcher,Wayne Mitzner,Rosanne Rouf,Shoji Hata,Daniel S Warren,Harry C Dietz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant mouse Wnt3a protein

Cell Differentiation - Recombinant mouse Wnt3a protein (AB81484)

Key facts

Purity

Endotoxin level

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Reconstitution

Storage buffer

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Reactivity data

Product details

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Product protocols

Target data

Publications (17)

Product promise