Recombinant mouse Wnt3a protein is a Mouse Full Length protein, in the 19 to 352 aa range with >75% purity, < 1 EU/µg endotoxin level and suitable for cell differentiation assays, HPLC, and SDS-PAGE. The predicted molecular weight of ab81484 protein is 37.4 kDa.
- Save time and ensure accurate results- use our recombiant Wnt3a protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Cited in over 15 publications
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Publications
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- Current research in neurobiology 5:1000952023Protocol for a Wnt reporter assay to measure its activity in human neural stem cells derived from induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristine Marie Yde Ohki,Natalie Monet Walter,Michelle Rickli,José Maria Salazar Campos,Anna Maria Werling,Christian Döring,Susanne Walitza,Edna GrünblattPubMed 37426743
- Methods in molecular biology (Clifton, N.J.) 2520:295-3072021Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmre Balbasi,Dersu Sezginmert,Ceren Alganatay,Nihal Terzi CizmeciogluPubMed 34611822
- Journal of cellular and molecular medicine 25:6963-69752021Fractalkine aggravates LPS-induced macrophage activation and acute kidney injury via Wnt/β-catenin signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiming Gong,Yan Jiang,Xiuhong Pan,Yanwu YouPubMed 34101346
- Angiogenesis 24:631-6452021Loss of endothelial glucocorticoid receptor promotes angiogenesis via upregulation of Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Liu,Han Zhou,Tiening Zhang,Xixiang Gao,Bo Tao,Hao Xing,Zhenwu Zhuang,Alan Dardik,Themis R Kyriakides,Julie E GoodwinPubMed 33650028
- American journal of physiology. Gastrointestinal and liver physiology 320:G627-G6432021Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, βPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKunrong Cheng,Shannon M Larabee,Mazen Tolaymat,Marie Hanscom,Aaron C Shang,Alyssa Schledwitz,Shien Hu,Cinthia B Drachenberg,Min Zhan,Ahmed Chahdi,Jean-Pierre RaufmanPubMed 33566751
- Molecular cancer 19:1182020LncRNA PVT1 promotes gemcitabine resistance of pancreatic cancer via activating Wnt/β-catenin and autophagy pathway through modulating the miR-619-5p/Pygo2 and miR-619-5p/ATG14 axes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCefan Zhou et. al.PubMed 32727463
- International journal of cell biology 2020:20459692020Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnisha Polley et. al.PubMed 32328102
- Journal of immunology (Baltimore, Md. : 1950) 203:2630-26432019Controls Germinal Center Reaction by Relaying a Wnt Signal to the Expression in Centroblasts during Humoral Immune Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeung Young Choi et. al.PubMed 31586036
- Science translational medicine 11:2019Calpain 9 as a therapeutic target in TGFβ-induced mesenchymal transition and fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid H Kim,James D Beckett,Varun Nagpal,Manuel A Seman-Senderos,Russell A Gould,Tyler J Creamer,Elena Gallo MacFarlane,Yichun Chen,Djahida Bedja,Jonathan T Butcher,Wayne Mitzner,Rosanne Rouf,Shoji Hata,Daniel S Warren,Harry C DietzPubMed 31316008
- Autophagy 15:1935-19532019Intestinal epithelial HMGB1 inhibits bacterial infection via STAT3 regulation of autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong-Guo Zhang,Xiaorong Zhu,Rong Lu,Jeannette S Messer,Yinglin Xia,Eugene B Chang,Jun SunPubMed 30894054
Key facts
- Purity
- >75% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Cell Culture
- Tags
- Tag free
- Applications
- HPLC, CellDiff, SDS-PAGE, CellAct
- Biologically active
- Yes
Amino acid sequence
S Y P I W W S L A V G P Q Y S S L S T Q P I L C A S I P G L V P K Q L R F C R N Y V E I M P S V A E G V K A G I Q E C Q H Q F R G R R W N C T T V S N S L A I F G P V L D K A T R E S A F V H A I A S A G V A F A V T R S C A E G S A A I C G C S S R L Q G S P G E G W K W G G C S E D I E F G G M V S R E F A D A R E N R P D A R S A M N R H N N E A G R Q A I A S H M H L K C K C H G L S G S C E V K T C W W S Q P D F R T I G D F L K D K Y D S A S E M V V E K H R E S R G W V E T L R P R Y T Y F K V P T E R D L V Y Y E A S P N F C E P N P E T G S F G T R D R T C N V S S H G I D G C D L L C C G R G H N A R T E R R R E K C H C V F H W C C Y V S C Q E C T R V Y D V H T C K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application CellDiff | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Due to glycosylation, ab81484 migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non-reducing conditions. |
Application CellAct | Reactivity Reacts | Dilution info - | Notes The expected ED50 for the induction of alkaline phosphatase production by CCL-226 cells is 0.4-0.6 ng/ml. |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites (PubMed:8299937). Required for normal morphogenesis of the developing neural tube (PubMed:8299937). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
Alternative names
Wnt-3a, Wnt3a, Protein Wnt-3a
Recommended products
Recombinant mouse Wnt3a protein is a Mouse Full Length protein, in the 19 to 352 aa range with >75% purity, < 1 EU/µg endotoxin level and suitable for cell differentiation assays, HPLC, and SDS-PAGE. The predicted molecular weight of ab81484 protein is 37.4 kDa.
- Save time and ensure accurate results- use our recombiant Wnt3a protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Cited in over 15 publications
Key facts
- Purity
- >75% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Cell Culture
- Tags
- Tag free
- Applications
- HPLC, CellDiff, SDS-PAGE, CellAct
- Biologically active
- Yes
- Biological activity
- The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.
The expected ED50 for this effect is 0.4-0.6 ng/ml.
- Accession
- P27467-1
- Animal free
- No
- Species
- Mouse
- Reconstitution
- reconstitute with water at 0.1mg/mL
- Concentration
- Storage buffer
Constituents: 13% 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
Sequence info
Amino acid sequence
- S Y P I W W S L A V G P Q Y S S L S T Q P I L C A S I P G L V P K Q L R F C R N Y V E I M P S V A E G V K A G I Q E C Q H Q F R G R R W N C T T V S N S L A I F G P V L D K A T R E S A F V H A I A S A G V A F A V T R S C A E G S A A I C G C S S R L Q G S P G E G W K W G G C S E D I E F G G M V S R E F A D A R E N R P D A R S A M N R H N N E A G R Q A I A S H M H L K C K C H G L S G S C E V K T C W W S Q P D F R T I G D F L K D K Y D S A S E M V V E K H R E S R G W V E T L R P R Y T Y F K V P T E R D L V Y Y E A S P N F C E P N P E T G S F G T R D R T C N V S S H G I D G C D L L C C G R G H N A R T E R R R E K C H C V F H W C C Y V S C Q E C T R V Y D V H T C K
- Accession
- P27467
- Protein length
- Full Length
- Amino acids
- 19 to 352
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Greater than 75% by HPLC analyses.
General info
- Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites (PubMed:8299937). Required for normal morphogenesis of the developing neural tube (PubMed:8299937). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
- Sequence similarities
Belongs to the Wnt family.
- Post-translational modifications
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- A few weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your results using our Recombinant mouse Wnt3a protein ab81484 as a positive control in SDS-PAGE.
Our bioactive recombinant mouse Wnt3 protein ab81484 is ideal to use in oncogenesis research and several developmental processes, including regulation of cell fate and patterning during embryogenesis.
The ab81484 mouse Wnt-3a is a monomeric glycoprotein. Due to glycosylation ab81484 migrates at an observed molecular weight of ~38-41 kDa by SDS-PAGE under non-reducing conditions.
Check out our protein gel staining guide for SDS-PAGE here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.
- Biological function summary
Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.
- Pathways
Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.
- Associated diseases and disorders
Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.
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1 product image
This image is courtesy of Emily Moorefield, Wake Forest Institute for Regenerative Medicine, NC, USACell Differentiation - Recombinant mouse Wnt3a protein (ab81484)
Human embryonic stem cells (HUES4) were differentiated as monolayer cultures on matrigel coated dishes using a protocol described in Kroon et al, Nat Biotechnol. (2008). Inhibin beta A (ab50051) and Wnt3a (ab81484) were added to cultures to direct differentiation towards definitive endoderm. This image was taken 3 days after induction, where the definitive endoderm marker FOXA2 (ab40874 in green) is abundant. Dapi staining is shown in blue. Magnification: 200x. Negative controls of fluorescently conjugated secondary antibodies only showed no staining.
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