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Recombinant mouse Wnt3a protein is a Mouse Full Length protein, in the 19 to 352 aa range, expressed in Cell Culture, with >75% purity, < 1 EU/µg endotoxin level and suitable for HPLC, CellDiff, SDS-PAGE, CellAct.
Alternative names=Protein Wnt-3a, Wnt3a, Wnt-3a
- Purity
>75% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Cell Culture
- Tags
Tag free
- Applications
HPLC, CellDiff, SDS-PAGE, CellAct
- Biologically active
Yes
- Sequence
S Y P I W W S L A V G P Q Y S S L S T Q P I L C A S I P G L V P K Q L R F C R N Y V E I M P S V A E G V K A G I Q E C Q H Q F R G R R W N C T T V S N S L A I F G P V L D K A T R E S A F V H A I A S A G V A F A V T R S C A E G S A A I C G C S S R L Q G S P G E G W K W G G C S E D I E F G G M V S R E F A D A R E N R P D A R S A M N R H N N E A G R Q A I A S H M H L K C K C H G L S G S C E V K T C W W S Q P D F R T I G D F L K D K Y D S A S E M V V E K H R E S R G W V E T L R P R Y T Y F K V P T E R D L V Y Y E A S P N F C E P N P E T G S F G T R D R T C N V S S H G I D G C D L L C C G R G H N A R T E R R R E K C H C V F H W C C Y V S C Q E C T R V Y D V H T C K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application CellDiff | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Due to glycosylation, ab81484 migrates at an apparent molecular weight of approximately 38.0-41.0 kDa by SDS-PAGE analysis under non-reducing conditions. |
Application CellAct | Reactivity Reacts | Dilution info - | Notes The expected ED50 for the induction of alkaline phosphatase production by CCL-226 cells is 0.4-0.6 ng/ml. |
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Recombinant mouse Wnt3a protein is a Mouse Full Length protein, in the 19 to 352 aa range, expressed in Cell Culture, with >75% purity, < 1 EU/µg endotoxin level and suitable for HPLC, CellDiff, SDS-PAGE, CellAct.
Alternative names=Protein Wnt-3a, Wnt3a, Wnt-3a
Key facts
- Purity
>75% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Cell Culture
- Tags
Tag free
- Applications
HPLC, CellDiff, SDS-PAGE, CellAct
- Biologically active
Yes
- Biological activity
The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.
The expected ED50 for this effect is 0.4-0.6 ng/ml.
- Amino acids
19 to 352
- Sequence
S Y P I W W S L A V G P Q Y S S L S T Q P I L C A S I P G L V P K Q L R F C R N Y V E I M P S V A E G V K A G I Q E C Q H Q F R G R R W N C T T V S N S L A I F G P V L D K A T R E S A F V H A I A S A G V A F A V T R S C A E G S A A I C G C S S R L Q G S P G E G W K W G G C S E D I E F G G M V S R E F A D A R E N R P D A R S A M N R H N N E A G R Q A I A S H M H L K C K C H G L S G S C E V K T C W W S Q P D F R T I G D F L K D K Y D S A S E M V V E K H R E S R G W V E T L R P R Y T Y F K V P T E R D L V Y Y E A S P N F C E P N P E T G S F G T R D R T C N V S S H G I D G C D L L C C G R G H N A R T E R R R E K C H C V F H W C C Y V S C Q E C T R V Y D V H T C K
- Accession
- P27467-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Mouse
- Reconstitution
Reconstitute to a concentration of 0.1 mg/mL with ddH2O. Store at 4°C for up to 1 week or dilute further in a buffer containing a carrier protein or stabilizer and store working aliquots at -20°C to -80°C for up to 3 months.
- Concentration
- Storage buffer
Constituents: 13% CHAPS
Specifications
- Form
Lyophilized
- Additional notes
Greater than 75% by HPLC analyses.
General info
- Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites (PubMed:8299937). Required for normal morphogenesis of the developing neural tube (PubMed:8299937). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
- Sequence similarities
Belongs to the Wnt family.
- Post-translational modifications
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT3A.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
A few weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Endotoxin level is less than 0.2 ng per μg (2EU/μg).
Formulation: Sterile filtered through a 0.2 micron filter. Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 500mM NaCl + 0.5% CHAPS.
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