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Recombinant Mouse Wnt8b protein (Tagged) is a Mouse Full Length protein, in the 22 to 350 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

W S V N N F L M T G P K A Y L V Y S S S V A A G A Q S G I E E C K Y Q F A W D R W N C P E R A L Q L S S H G G L R S A N R E T A F V H A I S S A G V M Y T L T R N C S L G D F D N C G C D D S R N G Q L G G Q G W L W G G C S D N V G F G E A I S K Q F V D A L E T G Q D A R A A M N L H N N E A G R K A V K G T M K R T C K C H G V S G S C T T Q T C W L Q L P E F R E V G A H L K E K Y H A A L K V D L L Q G A G N S A A G R G A I A D T F R S I S T R E L V H L E D S P D Y C L E N K T L G L L G T E G R E C L R R G R A L G R W E R R S C R R L C G D C G L A V E E R R A E T V S S C N C K F H W C C A V R C E Q C R R R V T K Y F C S R A E R P P R G A A H K P G K N S

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

Alternative names

Recommended products

Recombinant Mouse Wnt8b protein (Tagged) is a Mouse Full Length protein, in the 22 to 350 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
Q9WUD6-1
Animal free

No

Species

Mouse

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

W S V N N F L M T G P K A Y L V Y S S S V A A G A Q S G I E E C K Y Q F A W D R W N C P E R A L Q L S S H G G L R S A N R E T A F V H A I S S A G V M Y T L T R N C S L G D F D N C G C D D S R N G Q L G G Q G W L W G G C S D N V G F G E A I S K Q F V D A L E T G Q D A R A A M N L H N N E A G R K A V K G T M K R T C K C H G V S G S C T T Q T C W L Q L P E F R E V G A H L K E K Y H A A L K V D L L Q G A G N S A A G R G A I A D T F R S I S T R E L V H L E D S P D Y C L E N K T L G L L G T E G R E C L R R G R A L G R W E R R S C R R L C G D C G L A V E E R R A E T V S S C N C K F H W C C A V R C E Q C R R R V T K Y F C S R A E R P P R G A A H K P G K N S

Accession

Q9WUD6

Protein length

Full Length

Predicted molecular weight

56.2 kDa

Amino acids

22 to 350

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

Sequence similarities

Belongs to the Wnt family.

Post-translational modifications

Palmitoleoylation is required for efficient binding to frizzled receptors (By similarity). Depalmitoleoylation leads to Wnt signaling pathway inhibition (By similarity).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Wnt8b known as Wingless-type MMTV integration site family member 8B is a secreted protein involved in the Wnt signaling pathway. Its molecular weight is approximately 37 kDa. It is expressed in several tissues including the developing central nervous system and somites during embryogenesis. Wnt8b belongs to the family of Wnt proteins which play a role in cell communication and gene expression regulation.

Biological function summary

Wnt8b has a significant role in the regulation of cell fate and patterning during development. It acts as a morphogen influencing the gradient-mediated processes that define tissue differentiation. As part of the canonical Wnt signaling pathway Wnt8b binds to the Frizzled receptors and co-receptors like LRP5/6 leading to stabilization of the beta-catenin complex in the cytoplasm. This beta-catenin complex translocates to the nucleus where it affects the transcription of target genes.

Pathways

Wnt8b is involved in two major signaling pathways: the canonical Wnt/beta-catenin pathway and the planar cell polarity pathway. In the Wnt/beta-catenin pathway Wnt8b interacts with proteins like Dishevelled affecting downstream effectors such as beta-catenin. In the planar cell polarity pathway Wnt8b influences cellular orientation and movement through interactions with proteins such as Rho and Rac contributing to tissue polarity and motility.

Associated diseases and disorders

Wnt8b is associated with conditions like cancer and neurodevelopmental disorders. Aberrant Wnt8b expression can lead to tumor progression and metastasis with connections to oncogenes such as MYC and CTNNB1. In neurodevelopmental disorders disruptions in Wnt8b signaling can impact brain development and function relating to proteins that mediate synaptic communication and plasticity. Understanding these connections helps to elucidate the role of Wnt8b in pathological states.

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