Recombinant Mouse ZnT-8 protein is a Mouse Full Length protein, in the 1 to 367 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M E F L E R T Y L V N D Q A T K M Y A F P L D R E L R Q K P V N K D Q C P G D R P E H P E A G G I Y H C H N S A K A T G N R S S K Q A H A K W R L C A A S A I C F I F M V A E V V G G H V A G S L A I L T D A A H L L I D L T S F L L S L F S L W L S S R P P S K R L T F G W Y R A E I L G A L L S V L C I W V V T G V L L Y L A C E R L L Y P D Y Q I Q A G I M I T V S G C A V A A N I V L T M I L H Q R N F G Y N H K D V Q A N A S V R A A F V H A L G D V F Q S I S V L I S A L I I Y F K P D Y K I A D P V C T F I F S I L V L A S T V M I L K D F S I L L M E G V P K G L S Y N S V K E I I L A V D G V I S V H S L H I W S L T V N Q V I L S V H V A T A A S Q D S Q S V R T G I A Q A L S S F D L H S L T I Q I E S A A D Q D P S C L L C E D P Q D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.
Alternative names
Znt8, Slc30a8, Proton-coupled zinc antiporter SLC30A8, Solute carrier family 30 member 8, Zinc transporter 8, ZnT-8
Recommended products
Recombinant Mouse ZnT-8 protein is a Mouse Full Length protein, in the 1 to 367 aa range, expressed in Cell free, with >=85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Cell free
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q8BGG0-1
- Animal free
- Yes
- Species
- Mouse
- Concentration
- Storage buffer
pH: 7.4 - 8
Constituents: 6% Trehalose, 0.87% Sodium chloride, 0.24% Tris, 0.05% 2-Octadecoxyethanol
Sequence info
Amino acid sequence
- M E F L E R T Y L V N D Q A T K M Y A F P L D R E L R Q K P V N K D Q C P G D R P E H P E A G G I Y H C H N S A K A T G N R S S K Q A H A K W R L C A A S A I C F I F M V A E V V G G H V A G S L A I L T D A A H L L I D L T S F L L S L F S L W L S S R P P S K R L T F G W Y R A E I L G A L L S V L C I W V V T G V L L Y L A C E R L L Y P D Y Q I Q A G I M I T V S G C A V A A N I V L T M I L H Q R N F G Y N H K D V Q A N A S V R A A F V H A L G D V F Q S I S V L I S A L I I Y F K P D Y K I A D P V C T F I F S I L V L A S T V M I L K D F S I L L M E G V P K G L S Y N S V K E I I L A V D G V I S V H S L H I W S L T V N Q V I L S V H V A T A A S Q D S Q S V R T G I A Q A L S S F D L H S L T I Q I E S A A D Q D P S C L L C E D P Q D
- Accession
- Q8BGG0
- Protein length
- Full Length
- Predicted molecular weight
- 43 kDa
- Amino acids
- 1 to 367
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Proton-coupled zinc ion antiporter mediating the entry of zinc into the lumen of pancreatic beta cell secretory granules, thereby regulating insulin secretion.
- Sequence similarities
Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Zinc transporter 8 (ZnT-8) also known as SLC30A8 is a protein that functions as a zinc transporter located in the membrane of insulin secretory vesicles in pancreatic beta cells. The molecular mass of ZnT-8 is approximately 51 kDa. Expression of ZnT-8 has been observed predominantly in the pancreas with significant presence in islet cells. ZnT-8 facilitates the movement of zinc ions from the cytoplasm into insulin granules which is essential for proper insulin crystallization and storage.
- Biological function summary
Zinc plays an important role in insulin maturation and secretion and ZnT-8 contributes to these processes by regulating zinc ion homeostasis within the pancreatic beta cells. ZnT-8 is not part of a larger complex but operates alongside other zinc transporters to maintain zinc balance. This transporter ensures that zinc levels within insulin granules achieve conditions necessary for effective packaging and release of insulin.
- Pathways
ZnT-8 is directly involved in regulating glucose homeostasis and insulin signaling pathways. The protein plays a significant part in the insulin secretion pathway enabling the correct response to fluctuating blood glucose levels. ZnT-8 activity is closely related to glucose transporter type 2 (GLUT2) and other components of the insulin signaling pathway which ensures the maintenance of normal blood glucose levels through appropriate insulin action and secretion.
- Associated diseases and disorders
ZnT-8 has been closely associated with type 2 diabetes and autoimmune diabetes. Variants in the SLC30A8 gene which encodes ZnT-8 have been linked to altered risk of developing type 2 diabetes. This connection implicates ZnT-8 in the pathophysiology of impaired insulin function. Furthermore ZnT-8 is a significant autoantigen in autoimmune diabetes with antibodies against ZnT-8 (anti-ZnT8 antibodies) being detected in patients suggesting an immune response involving this protein in the disease process.
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1 product image
SDS-PAGE - Recombinant Mouse ZnT-8 protein (ab289758)
SDS-PAGE analysis of ab289758
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