Recombinant Mumps virus wild-type, Gloucester strain, nucleocapsid protein is a Mumps virus Miyahara vaccine Full Length protein, expressed in Saccharomyces cerevisiae, with >95% purity and suitable for ELISA, WB, SDS-PAGE.
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- Vaccines 8:2020Protective Malaria Vaccine in Mice Based on the Circumsporozoite Protein Fused with the Mumps Nucleocapsid Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodolfo F Marques et. al.PubMed 32325874
- Epidemiology and infection 148:e172020Application of a fast and cost-effective 'three-in-one' MMR ELISA as a tool for surveying anti-MMR humoral immunity: the Hungarian experience.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Böröcz et. al.PubMed 32014073
- IEEE journal of solid-state circuits 53:2054-20642018High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander C Sun et. al.PubMed 30559530
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- ELISA, WB, SDS-PAGE
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info 5 µg/mL | Notes - |
Application WB | Reactivity Reacts | Dilution info 1 µg/mL | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info 1.2 µg/mL | Notes - |
Target data
Function
Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases (By similarity). The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity).
Additional Targets
Mumps virus wild-type, Gloucester strain, nucleocapsid
Alternative names
NP, N, Nucleoprotein, Nucleocapsid protein, Protein N
Recommended products
Recombinant Mumps virus wild-type, Gloucester strain, nucleocapsid protein is a Mumps virus Miyahara vaccine Full Length protein, expressed in Saccharomyces cerevisiae, with >95% purity and suitable for ELISA, WB, SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Saccharomyces cerevisiae
- Tags
- Tag free
- Applications
- ELISA, WB, SDS-PAGE
- Biologically active
- No
- Accession
- P21186-1
- Animal free
- No
- Species
- Mumps virus Miyahara vaccine
- Reconstitution
- Reconstitute in water, 50% glycerol
- Concentration
- Storage buffer
Constituents: PBS
Specifications
- Form
- Lyophilized
- Additional notes
ab74560 is purified by ultracentifugation.
General info
- Function
Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases (By similarity). The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity).
- Sequence similarities
Belongs to the paramyxoviruses nucleocapsid family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Reconstitute for long term storage
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Mumps virus wild-type Gloucester strain nucleocapsid protein plays an important role in the encapsidation of the viral RNA genome which facilitates the replication process of the virus. This protein also known as NP has a mass of approximately 57 kDa and expresses itself in infected host cells where it forms a nucleocapsid structure. The nucleocapsid protein interacts tightly with viral RNA protecting it from host nucleases and serving as a template for viral RNA synthesis.
- Biological function summary
The nucleocapsid protein of the Mumps virus Gloucester strain functions as a primary structural component of the virus. It is not a part of a multi-protein complex outside of its encapsulation role although it forms oligomeric structures important for its function. By interacting with other viral proteins like phosphoprotein (P) and large protein (L) it facilitates the localization and assembly of viral components which is essential for virus replication and transcription processes.
- Pathways
This nucleocapsid protein interacts directly with the virus replication and transcription machinery. It is situated within the Mumps virus life cycle pathway where it drives the replication of viral RNA. The nucleocapsid protein closely associates with the P and L proteins in these pathways enabling efficient synthesis of new viral genomes and the production of viral mRNA required for the assembly of new virions.
- Associated diseases and disorders
The nucleocapsid protein of the Mumps virus Gloucester strain is central to the pathology of mumps disease. The spread and replication of the virus within the host cells lead to systemic infections characterized by swelling of the salivary glands. There is also a risk of complications such as meningitis and encephalitis. The nucleocapsid protein's interaction with the viral P protein remains key to understanding its role in mumps-related pathogenesis.
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1 product image
SDS-PAGE - Recombinant Mumps virus wild-type, Gloucester strain, nucleocapsid protein (ab74560)
SDS-PAGE showing ab74560 at approximately 66kDa. (1.2 μg/lane).
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