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AB83471

Recombinant Mycobacterium tuberculosis Ag85B protein

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(1 Publication)

Recombinant Mycobacterium tuberculosis Ag85B protein is a Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE. The predicted molecular weight of ab83471 protein is 30 kDa.

- Save time and ensure accurate results - use our Ag85B protein as a control

View Alternative Names

30 kDa extracellular protein, Antigen 85 complex B, Mycobacterium, Mycolyl transferase 85B

1 Images
SDS-PAGE - Recombinant Mycobacterium tuberculosis Ag85B protein (AB83471)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mycobacterium tuberculosis Ag85B protein (AB83471)

SDS Page 12% polyacrylamide gel with 5µg ab83471 stained with Coomassie Blue in lane 2 compared with the molecular marker in lane 1.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Animal free

No

Carrier free

No

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.328% Sodium phosphate, 0.284% Disodium hydrogenorthophosphate, 0.24% Sodium dihydrogen phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our recombinant Mycobacterium tuberculosis Ag85B protein ab83471 as a positive control in SDS-PAGE.


Check out our protein gel staining guide for SDS-PAGE here
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Sequence info

[{"sequence":"MTDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGGNNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETFLTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGDAGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKFQDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG","proteinLength":"Full Length","predictedMolecularWeight":"30 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10153","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Mycobacterium tuberculosis Ag85B protein also known as Antigen 85B or FBPA is a component of the Ag85 complex. This protein has a molecular mass of approximately 31 kDa. The Ag85B protein plays an important mechanical role by catalyzing the formation of the cord factor a major glycolipid in the cell wall of Mycobacterium tuberculosis. It is expressed in the mycobacterium's cell wall contributing to the virulence of this pathogen by promoting the invasion and survival within the host.
Biological function summary

The Ag85B protein serves in the assembly and maintenance of the mycobacterial cell wall by synthesizing mycolate. It is a part of the Ag85 complex which includes Ag85A Ag85B and Ag85C ensuring accurate synthesis and export of essential components of the cell wall. Through its actions Ag85B is involved in interacting with the host immune system and is a target for vaccine development due to its immunogenic properties. Its presence is detected during histological examinations of infected tissues where it shows the interaction with host cells.

Pathways

The Ag85B protein functions within the mycolic acid biosynthesis pathway essential for maintaining the structure of the mycobacterial cell envelope. This protein interacts with Ag85A and Ag85C to orchestrate complex lipid synthesis required for Mycobacterium tuberculosis' survival. Ag85B's role in lipid metabolism makes it important in the pathogen's adaptation to the host environment by altering the cell wall to evade immune detection.

Ag85B is highly relevant to respiratory diseases like tuberculosis. This protein plays a role in the pathogen's ability to resist destruction by the host's immune system contributing to the chronic nature of tuberculosis. Additionally Ag85B has been investigated in the context of its potential utility in vaccines and diagnostic tests for Mycobacterium tuberculosis infections. Its interactions with other proteins in the Ag85 complex make it a focus of research for developing therapeutics to treat and manage tuberculosis.
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Specifications

Form

Lyophilized

Additional notes

Endotoxin Tested

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General info

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Product protocols

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Target data

See full target information Mycobacterium tuberculosis Ag85B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Allergologia et immunopathologia 51:27-35 PubMed36916085

2023

Triamcinolone acetonide induces the autophagy of Ag85B-treated WI-38 cells via SIRT1/FOXO3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Luo,Lei Zhou,Linzi Luo,Dan Feng,Yan Ding,Zhibin Lu,Ganjuan Nie,Liqiong Bai,Yangbao Xiao
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