Recombinant Mycobacterium tuberculosis Ag85B protein is a Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE. The predicted molecular weight of ab83471 protein is 30 kDa.
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- Allergologia et immunopathologia 51:27-352023Triamcinolone acetonide induces the autophagy of Ag85B-treated WI-38 cells via SIRT1/FOXO3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Luo,Lei Zhou,Linzi Luo,Dan Feng,Yan Ding,Zhibin Lu,Ganjuan Nie,Liqiong Bai,Yangbao XiaoPubMed 36916085
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M T D V S R K I R A W G R R L M I G T A A A V V L P G L V G L A G G A A T A G A F S R P G L P V E Y L Q V P S P S M G R D I K V Q F Q S G G N N S P A V Y L L D G L R A Q D D Y N G W D I N T P A F E W Y Y Q S G L S I V M P V G G Q S S F Y S D W Y S P A C G K A G C Q T Y K W E T F L T S E L P Q W L S A N R A V K P T G S A A I G L S M A G S S A M I L A A Y H P Q Q F I Y A G S L S A L L D P S Q G M G P S L I G L A M G D A G G Y K A A D M W G P S S D P A W E R N D P T Q Q I P K L V A N N T R L W V Y C G N G T P N E L G G A N I P A E F L E N F V R S S N L K F Q D A Y N A A G G H N A V F N F P P N G T H S W E Y W G A Q L N A M K G D L Q S S L G A G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
30 kDa extracellular protein, Antigen 85 complex B, Mycobacterium, Mycolyl transferase 85B
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Recombinant Mycobacterium tuberculosis Ag85B protein is a Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE. The predicted molecular weight of ab83471 protein is 30 kDa.
- Save time and ensure accurate results - use our Ag85B protein as a control
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Animal free
- No
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 2.9% Sodium chloride, 0.328% Sodium phosphate, 0.284% Disodium hydrogenorthophosphate, 0.24% Sodium dihydrogen phosphate
Sequence info
Amino acid sequence
- M T D V S R K I R A W G R R L M I G T A A A V V L P G L V G L A G G A A T A G A F S R P G L P V E Y L Q V P S P S M G R D I K V Q F Q S G G N N S P A V Y L L D G L R A Q D D Y N G W D I N T P A F E W Y Y Q S G L S I V M P V G G Q S S F Y S D W Y S P A C G K A G C Q T Y K W E T F L T S E L P Q W L S A N R A V K P T G S A A I G L S M A G S S A M I L A A Y H P Q Q F I Y A G S L S A L L D P S Q G M G P S L I G L A M G D A G G Y K A A D M W G P S S D P A W E R N D P T Q Q I P K L V A N N T R L W V Y C G N G T P N E L G G A N I P A E F L E N F V R S S N L K F Q D A Y N A A G G H N A V F N F P P N G T H S W E Y W G A Q L N A M K G D L Q S S L G A G
- Accession
- P10153
- Protein length
- Full Length
- Predicted molecular weight
- 30 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
Endotoxin Tested
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Mycobacterium tuberculosis Ag85B protein also known as Antigen 85B or FBPA is a component of the Ag85 complex. This protein has a molecular mass of approximately 31 kDa. The Ag85B protein plays an important mechanical role by catalyzing the formation of the cord factor a major glycolipid in the cell wall of Mycobacterium tuberculosis. It is expressed in the mycobacterium's cell wall contributing to the virulence of this pathogen by promoting the invasion and survival within the host.
- Biological function summary
The Ag85B protein serves in the assembly and maintenance of the mycobacterial cell wall by synthesizing mycolate. It is a part of the Ag85 complex which includes Ag85A Ag85B and Ag85C ensuring accurate synthesis and export of essential components of the cell wall. Through its actions Ag85B is involved in interacting with the host immune system and is a target for vaccine development due to its immunogenic properties. Its presence is detected during histological examinations of infected tissues where it shows the interaction with host cells.
- Pathways
The Ag85B protein functions within the mycolic acid biosynthesis pathway essential for maintaining the structure of the mycobacterial cell envelope. This protein interacts with Ag85A and Ag85C to orchestrate complex lipid synthesis required for Mycobacterium tuberculosis' survival. Ag85B's role in lipid metabolism makes it important in the pathogen's adaptation to the host environment by altering the cell wall to evade immune detection.
- Associated diseases and disorders
Ag85B is highly relevant to respiratory diseases like tuberculosis. This protein plays a role in the pathogen's ability to resist destruction by the host's immune system contributing to the chronic nature of tuberculosis. Additionally Ag85B has been investigated in the context of its potential utility in vaccines and diagnostic tests for Mycobacterium tuberculosis infections. Its interactions with other proteins in the Ag85 complex make it a focus of research for developing therapeutics to treat and manage tuberculosis.
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1 product image
SDS-PAGE - Recombinant Mycobacterium tuberculosis Ag85B protein (ab83471)
SDS Page 12% polyacrylamide gel with 5µg ab83471 stained with Coomassie Blue in lane 2 compared with the molecular marker in lane 1.
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