JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB318949

Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) is a Mycobacterium tuberculosis CDC1551 Full Length protein, expressed in Escherichia coli, with >95%, 0.005 EU/µg endotoxin level, suitable for Mass Spec, SDS-PAGE.

View Alternative Names

MT1934, fbpB, Diacylglycerol acyltransferase/mycolyltransferase Ag85B, DGAT, 30 kDa extracellular protein, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex B, Extracellular alpha-antigen, Fibronectin-binding protein B, 85B, Ag85B, Fbps B

2 Images
Mass Spectrometry - Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) (AB318949)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) (AB318949)

Mass determination by ESI-TOF. Predicted MW is 32750 Da (+/- 10 Da by ESI-TOF). Observed MW is 32618.46 Da.

SDS-PAGE - Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) (AB318949)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) (AB318949)

SDS-PAGE analysis of ab318949 under reducing conditions for 2ug protein.

Key facts

Purity

>95% HPLC

Endotoxin level

0.005 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Mass Spectrometry

LC-MS/MS

Accession

P9WQP0

Animal free

Yes

Carrier free

No

Species

Mycobacterium tuberculosis CDC1551

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Complementary Products

Proteins & Peptides

AB83471

Recombinant Mycobacterium tuberculosis Ag85B protein

SDS-PAGE - Recombinant Mycobacterium tuberculosis Ag85B protein (AB83471)

  • 0
  • 0
Proteins & Peptides

AB226277

Recombinant Mycobacterium tuberculosis MPT64 protein (His tag)

Mass Spectrometry - Recombinant Mycobacterium tuberculosis MPT64 protein (His tag) (AB226277)

  • 0
  • 0
Proteins & Peptides

AB124574

Recombinant Mycobacterium tuberculosis ESAT6 protein

SDS-PAGE - Recombinant Mycobacterium tuberculosis ESAT6 protein (AB124574)

  • 5
  • 1
Primary Antibodies

AB179483

Anti-HIF-1 alpha antibody [EPR16897]

RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-HIF-1 alpha antibody [EPR16897] (AB179483)

  • 4
  • 9
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"FSRPGLPVEYLQVPSPSMGRDIKVQFQSGGNNSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSIVMPVGGQSSFYSDWYSPACGKAGCQTYKWETFLTSELPQWLSANRAVKPTGSAAIGLSMAGSSAMILAAYHPQQFIYAGSLSALLDPSQGMGPSLIGLAMGDAGGYKAADMWGPSSDPAWERNDPTQQIPKLVANNTRLWVYCGNGTPNELGGANIPAEFLENFVRSSNLKFQDAYNAAGGHNAVFNFPPNGTHSWEYWGAQLNAMKGDLQSSLGAG","proteinLength":"Full Length","predictedMolecularWeight":"32.75 kDa","actualMolecularWeight":"32.62 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P9WQP0","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Mycobacterium tuberculosis Ag85 also called Ag85 complex or Ag-85 consists of a series of related proteins secreted by Mycobacterium tuberculosis including Ag85A Ag85B and Ag85C. This protein family plays a central role in the pathogen's ability to synthesize mycolic acids. The Ag85 complex has a molecular mass of around 30 to 32 kDa and is abundantly expressed on the surface of the Mycobacterium tuberculosis cell wall.
Biological function summary

The Ag85 complex facilitates the synthesis and transfer of mycolic acids essential constituents of the mycobacterial cell wall's lipid-rich structure. The Ag85 proteins participate in forming this barrier allowing Mycobacterium tuberculosis to resist host immune defenses. This functionality is linked to its involvement in the mycolyltransferase enzyme system which operates in collaboration with several other proteins necessary for full cell wall biosynthesis.

Pathways

The Ag85 complex significantly contributes to the biosynthetic route of mycolic acids an integral component of mycobacterial cell walls. This pathway includes interactions with other proteins like Pks13 and Mycobacterial arabinosyltransferase (EmbB) both important for the complex architectural integrity and viability of the mycobacterial cell wall. This biosynthesis pathway ensures proper assembly and maintenance of the cell envelope enhancing the persistence of the bacteria.

The Ag85 proteins are critical in the pathogenesis of tuberculosis where their activity supports the survival of the bacteria within host macrophages. Ag85 complexes are considered potential targets for diagnostic and therapeutic applications against tuberculosis. Additionally in leprosy another disease caused by mycobacteria proteins of the Ag85 complex have shown cross-reactivity emphasizing their significant role in bacterial viability and immune response evasion.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).

Sequence similarities

Belongs to the mycobacterial A85 antigen family.

style
left-column

Product protocols

style
left-column

Target data

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
See full target information fbpB
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com