Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) is a Mycobacterium tuberculosis CDC1551 Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
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Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- = 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
F S R P G L P V E Y L Q V P S P S M G R D I K V Q F Q S G G N N S P A V Y L L D G L R A Q D D Y N G W D I N T P A F E W Y Y Q S G L S I V M P V G G Q S S F Y S D W Y S P A C G K A G C Q T Y K W E T F L T S E L P Q W L S A N R A V K P T G S A A I G L S M A G S S A M I L A A Y H P Q Q F I Y A G S L S A L L D P S Q G M G P S L I G L A M G D A G G Y K A A D M W G P S S D P A W E R N D P T Q Q I P K L V A N N T R L W V Y C G N G T P N E L G G A N I P A E F L E N F V R S S N L K F Q D A Y N A A G G H N A V F N F P P N G T H S W E Y W G A Q L N A M K G D L Q S S L G A G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
Alternative names
MT1934, fbpB, Diacylglycerol acyltransferase/mycolyltransferase Ag85B, DGAT, 30 kDa extracellular protein, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex B, Extracellular alpha-antigen, Fibronectin-binding protein B, 85B, Ag85B, Fbps B
Recommended products
Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) is a Mycobacterium tuberculosis CDC1551 Full Length protein, expressed in Escherichia coli, with >=95% purity, = 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- = 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P9WQP0-1
- Animal free
- Yes
- Species
- Mycobacterium tuberculosis CDC1551
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% Glycerol (glycerin, glycerine), 10% PBS
Sequence info
Amino acid sequence
- F S R P G L P V E Y L Q V P S P S M G R D I K V Q F Q S G G N N S P A V Y L L D G L R A Q D D Y N G W D I N T P A F E W Y Y Q S G L S I V M P V G G Q S S F Y S D W Y S P A C G K A G C Q T Y K W E T F L T S E L P Q W L S A N R A V K P T G S A A I G L S M A G S S A M I L A A Y H P Q Q F I Y A G S L S A L L D P S Q G M G P S L I G L A M G D A G G Y K A A D M W G P S S D P A W E R N D P T Q Q I P K L V A N N T R L W V Y C G N G T P N E L G G A N I P A E F L E N F V R S S N L K F Q D A Y N A A G G H N A V F N F P P N G T H S W E Y W G A Q L N A M K G D L Q S S L G A G
- Accession
- P9WQP0
- Protein length
- Full Length
- Predicted molecular weight
- 32.75 kDa
- Actual molecular weight
- 32.62 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
- Sequence similarities
Belongs to the mycobacterial A85 antigen family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Mycobacterium tuberculosis Ag85 also called Ag85 complex or Ag-85 consists of a series of related proteins secreted by Mycobacterium tuberculosis including Ag85A Ag85B and Ag85C. This protein family plays a central role in the pathogen's ability to synthesize mycolic acids. The Ag85 complex has a molecular mass of around 30 to 32 kDa and is abundantly expressed on the surface of the Mycobacterium tuberculosis cell wall.
- Biological function summary
The Ag85 complex facilitates the synthesis and transfer of mycolic acids essential constituents of the mycobacterial cell wall's lipid-rich structure. The Ag85 proteins participate in forming this barrier allowing Mycobacterium tuberculosis to resist host immune defenses. This functionality is linked to its involvement in the mycolyltransferase enzyme system which operates in collaboration with several other proteins necessary for full cell wall biosynthesis.
- Pathways
The Ag85 complex significantly contributes to the biosynthetic route of mycolic acids an integral component of mycobacterial cell walls. This pathway includes interactions with other proteins like Pks13 and Mycobacterial arabinosyltransferase (EmbB) both important for the complex architectural integrity and viability of the mycobacterial cell wall. This biosynthesis pathway ensures proper assembly and maintenance of the cell envelope enhancing the persistence of the bacteria.
- Associated diseases and disorders
The Ag85 proteins are critical in the pathogenesis of tuberculosis where their activity supports the survival of the bacteria within host macrophages. Ag85 complexes are considered potential targets for diagnostic and therapeutic applications against tuberculosis. Additionally in leprosy another disease caused by mycobacteria proteins of the Ag85 complex have shown cross-reactivity emphasizing their significant role in bacterial viability and immune response evasion.
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2 product images
SDS-PAGE - Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) (ab318949)
SDS-PAGE analysis of ab318949 under reducing conditions for 2ug protein.
Mass Spectrometry - Recombinant Mycobacterium tuberculosis CDC1551 Ag85 Protein (His tag) (ab318949)
Mass determination by ESI-TOF.
Predicted MW is 32750 Da (+/- 10 Da by ESI-TOF). Observed MW is 32618.46 Da.
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