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AB124604

Recombinant Mycobacterium tuberculosis TB Ag85A protein (His tag C-Terminus)

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(2 Publications)

Recombinant Mycobacterium tuberculosis TB Ag85A protein (His tag C-Terminus) is a Mycobacterium tuberculosis H37Rv Full Length protein, in the 44 to 338 aa range, expressed in Baculovirus infected BTI-TN-5B1-4 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

mpt44, Rv3804c, MTV026.09c, fbpA, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex A, Fibronectin-binding protein A, 85A, Ag85A, Fbps A

1 Images
SDS-PAGE - Recombinant Mycobacterium tuberculosis TB Ag85A protein (His tag C-Terminus) (AB124604)
  • SDS-PAGE

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SDS-PAGE - Recombinant Mycobacterium tuberculosis TB Ag85A protein (His tag C-Terminus) (AB124604)

15% SDS-PAGE showing ab124604 (3 µg).

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected BTI-TN-5B1-4 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P9WQP3

Animal free

No

Carrier free

No

Species

Mycobacterium tuberculosis H37Rv

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ADPAFSRPGLPVEYLQVPSPSMGRDIKVQFQSGGANSPALYLLDGLRAQDDFSGWDINTPAFEWYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKWETFLTSELPGWLQANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAMGPTLIGLAMGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWVYCGNGKPSDLGGNNLPAKFLEGFVRTSNIKFQDAYNAGGGHNGVFDFPDSGTHSWEYWGAQLNAMKPDLQRALGATPNTGPAPQGAHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"32.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":338,"aminoAcidStart":44,"nature":"Recombinant","expressionSystem":"Baculovirus infected BTI-TN-5B1-4 cells","accessionNumber":"P9WQP3","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TB Ag85A also known as Antigen 85A is an important protein in Mycobacterium tuberculosis. It has a molecular mass of approximately 30-32 kDa. TB Ag85A is part of the antigen 85 complex which consists of proteins Ag85A Ag85B and Ag85C. This protein actively participates in mycobacterial cell wall synthesis. It catalyzes the transfer of mycolic acids to the cell wall arabinogalactan contributing to the bacteria's protective barrier. Expression occurs heavily in the cell envelope of Mycobacterium tuberculosis enabling vital interactions with the host's immune cells.
Biological function summary

TB Ag85A plays a significant role in the survival and virulence of Mycobacterium tuberculosis within host macrophages. It is part of the antigen 85 complex an important element in the synthesis of the mycobacterial cell envelope. This complex facilitates the interconversion of trehalose monomycolate and trehalose dimycolate critical for granuloma formation. The antigen 85 complex interferes with the host's immune response aiding the persistence of the bacterium. The synthesis and secretion of TB Ag85A by Mycobacterium tuberculosis allow it to adjust and survive hostile intra-host environments.

Pathways

TB Ag85A participates in the lipid biosynthesis pathway integral to cell envelope formation. This pathway plays a fundamental role in maintaining mycobacterial cell wall integrity. TB Ag85A is linked with proteins like Ag85B and Ag85C which are components of the same antigen 85 complex. These proteins work together within the pathway contributing to the robust defense system of Mycobacterium tuberculosis which complicates treatment and eradication efforts.

TB Ag85A is closely connected to tuberculosis a severe infectious disease. The protein's role in cell wall synthesis and immune evasion links it to the pathogen's ability to persist in the host. TB Ag85A's interactions with host immune defenses allow Mycobacterium tuberculosis to survive and proliferate in the lungs. Additionally studies propose TB Ag85A as a potential target for developing vaccines against tuberculosis given its role in infection and immune evasion. Researchers are exploring its connections to Ag85B and Ag85C for novel therapeutic interventions in tuberculosis management.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26 : 0-CoA over C18 : 1-CoA.

Sequence similarities

Belongs to the mycobacterial A85 antigen family.

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Product protocols

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Target data

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26 : 0-CoA over C18 : 1-CoA.
See full target information fbpA

Additional targets

TB Ag85A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadh8228 PubMed37738349

2023

Temporal landscape of mitochondrial proteostasis governed by the UPR.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Uoselis,Runa Lindblom,Wai Kit Lam,Catharina J Küng,Marvin Skulsuppaisarn,Grace Khuu,Thanh N Nguyen,Danielle L Rudler,Aleksandra Filipovska,Ralf B Schittenhelm,Michael Lazarou

Frontiers in microbiology 8:1263 PubMed28744263

2017

Recombinant Invasive Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-γ, IL-6, and TNF-α Cytokines after Intranasal Immunization.

Applications

FuncS

Species

Mouse

Pamela Mancha-Agresti,Camila Prosperi de Castro,Janete S C Dos Santos,Maíra A Araujo,Vanessa B Pereira,Jean G LeBlanc,Sophie Y Leclercq,Vasco Azevedo
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