Recombinant Mycobacterium tuberculosis TB Ag85A protein is a Mycobacterium tuberculosis H37Rv Full Length protein, in the 44 to 338 aa range, expressed in Baculovirus infected BTI-TN-5B1-4, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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- Science advances 9:eadh82282023Temporal landscape of mitochondrial proteostasis governed by the UPR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLouise Uoselis,Runa Lindblom,Wai Kit Lam,Catharina J Küng,Marvin Skulsuppaisarn,Grace Khuu,Thanh N Nguyen,Danielle L Rudler,Aleksandra Filipovska,Ralf B Schittenhelm,Michael LazarouPubMed 37738349
- Frontiers in microbiology 8:12632017Recombinant Invasive Carrying a DNA Vaccine Coding the Ag85A Antigen Increases INF-γ, IL-6, and TNF-α Cytokines after Intranasal Immunization.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected BTI-TN-5B1-4 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A D P A F S R P G L P V E Y L Q V P S P S M G R D I K V Q F Q S G G A N S P A L Y L L D G L R A Q D D F S G W D I N T P A F E W Y D Q S G L S V V M P V G G Q S S F Y S D W Y Q P A C G K A G C Q T Y K W E T F L T S E L P G W L Q A N R H V K P T G S A V V G L S M A A S S A L T L A I Y H P Q Q F V Y A G A M S G L L D P S Q A M G P T L I G L A M G D A G G Y K A S D M W G P K E D P A W Q R N D P L L N V G K L I A N N T R V W V Y C G N G K P S D L G G N N L P A K F L E G F V R T S N I K F Q D A Y N A G G G H N G V F D F P D S G T H S W E Y W G A Q L N A M K P D L Q R A L G A T P N T G P A P Q G A H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.
Additional Targets
TB Ag85A
Alternative names
mpt44, Rv3804c, MTV026.09c, fbpA, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Antigen 85 complex A, Fibronectin-binding protein A, 85A, Ag85A, Fbps A
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Recombinant Mycobacterium tuberculosis TB Ag85A protein is a Mycobacterium tuberculosis H37Rv Full Length protein, in the 44 to 338 aa range, expressed in Baculovirus infected BTI-TN-5B1-4, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Baculovirus infected BTI-TN-5B1-4 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P9WQP3-1
- Animal free
- No
- Species
- Mycobacterium tuberculosis H37Rv
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl
Sequence info
Amino acid sequence
- A D P A F S R P G L P V E Y L Q V P S P S M G R D I K V Q F Q S G G A N S P A L Y L L D G L R A Q D D F S G W D I N T P A F E W Y D Q S G L S V V M P V G G Q S S F Y S D W Y Q P A C G K A G C Q T Y K W E T F L T S E L P G W L Q A N R H V K P T G S A V V G L S M A A S S A L T L A I Y H P Q Q F V Y A G A M S G L L D P S Q A M G P T L I G L A M G D A G G Y K A S D M W G P K E D P A W Q R N D P L L N V G K L I A N N T R V W V Y C G N G K P S D L G G N N L P A K F L E G F V R T S N I K F Q D A Y N A G G G H N G V F D F P D S G T H S W E Y W G A Q L N A M K P D L Q R A L G A T P N T G P A P Q G A H H H H H H
- Accession
- P9WQP3
- Protein length
- Full Length
- Predicted molecular weight
- 32.8 kDa
- Amino acids
- 44 to 338
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.
- Sequence similarities
Belongs to the mycobacterial A85 antigen family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TB Ag85A also known as Antigen 85A is an important protein in Mycobacterium tuberculosis. It has a molecular mass of approximately 30-32 kDa. TB Ag85A is part of the antigen 85 complex which consists of proteins Ag85A Ag85B and Ag85C. This protein actively participates in mycobacterial cell wall synthesis. It catalyzes the transfer of mycolic acids to the cell wall arabinogalactan contributing to the bacteria's protective barrier. Expression occurs heavily in the cell envelope of Mycobacterium tuberculosis enabling vital interactions with the host's immune cells.
- Biological function summary
TB Ag85A plays a significant role in the survival and virulence of Mycobacterium tuberculosis within host macrophages. It is part of the antigen 85 complex an important element in the synthesis of the mycobacterial cell envelope. This complex facilitates the interconversion of trehalose monomycolate and trehalose dimycolate critical for granuloma formation. The antigen 85 complex interferes with the host's immune response aiding the persistence of the bacterium. The synthesis and secretion of TB Ag85A by Mycobacterium tuberculosis allow it to adjust and survive hostile intra-host environments.
- Pathways
TB Ag85A participates in the lipid biosynthesis pathway integral to cell envelope formation. This pathway plays a fundamental role in maintaining mycobacterial cell wall integrity. TB Ag85A is linked with proteins like Ag85B and Ag85C which are components of the same antigen 85 complex. These proteins work together within the pathway contributing to the robust defense system of Mycobacterium tuberculosis which complicates treatment and eradication efforts.
- Associated diseases and disorders
TB Ag85A is closely connected to tuberculosis a severe infectious disease. The protein's role in cell wall synthesis and immune evasion links it to the pathogen's ability to persist in the host. TB Ag85A's interactions with host immune defenses allow Mycobacterium tuberculosis to survive and proliferate in the lungs. Additionally studies propose TB Ag85A as a potential target for developing vaccines against tuberculosis given its role in infection and immune evasion. Researchers are exploring its connections to Ag85B and Ag85C for novel therapeutic interventions in tuberculosis management.
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SDS-PAGE - Recombinant Mycobacterium tuberculosis TB Ag85A protein (ab124604)
15% SDS-PAGE showing ab124604 (3 µg).
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