Recombinant Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 protein (Tagged) is a Tetronarce californica Fragment protein, in the 25 to 234 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
S E H E T R L V A N L L E N Y N K V I R P V E H H T H F V D I T V G L Q L I Q L I S V D E V N Q I V E T N V R L R Q Q W I D V R L R W N P A D Y G G I K K I R L P S D D V W L P D L V L Y N N A D G D F A I V H M T K L L L D Y T G K I M W T P P A I F K S Y C E I I V T H F P F D Q Q N C T M K L G I W T Y D G T K V S I S P E S D R P D L S T F M E S G E W V M K D Y R G W K H W V Y Y T C C P D T P Y L D I T Y H F I M Q R I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Upon acetylcholine binding, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Alternative names
Acetylcholine receptor subunit alpha, CHRNA1
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Recombinant Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 protein (Tagged) is a Tetronarce californica Fragment protein, in the 25 to 234 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P02710-1
- Animal free
- No
- Species
- Tetronarce californica
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- S E H E T R L V A N L L E N Y N K V I R P V E H H T H F V D I T V G L Q L I Q L I S V D E V N Q I V E T N V R L R Q Q W I D V R L R W N P A D Y G G I K K I R L P S D D V W L P D L V L Y N N A D G D F A I V H M T K L L L D Y T G K I M W T P P A I F K S Y C E I I V T H F P F D Q Q N C T M K L G I W T Y D G T K V S I S P E S D R P D L S T F M E S G E W V M K D Y R G W K H W V Y Y T C C P D T P Y L D I T Y H F I M Q R I
- Accession
- P02710
- Protein length
- Fragment
- Predicted molecular weight
- 40.8 kDa
- Amino acids
- 25 to 234
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Upon acetylcholine binding, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
- Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 1
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Nicotinic Acetylcholine Receptor alpha 1 also known as CHRNA1 is an essential component of the nicotinic acetylcholine receptor complex. This receptor an ion channel found in the neuromuscular junction is involved in the mechanical process of signal transmission between nerves and muscles. The protein facilitates the binding of acetylcholine a neurotransmitter which triggers an ion flow causing muscle contraction. The CHRNA1 subunit generally displays a mass of approximately 58 kDa and is widely expressed in muscle tissues.
- Biological function summary
The Nicotinic Acetylcholine Receptor alpha 1 has a fundamental role in neuromuscular communication. It is part of a pentameric complex in combination with other acetylcholine receptor subunits. When acetylcholine binds to the receptor the channel opens permitting cations like sodium and calcium to enter the muscle cell leading to depolarization and subsequent muscle contraction. This biological process is essential for voluntary muscle movement.
- Pathways
The Nicotinic Acetylcholine Receptor alpha 1 is involved in the neuromuscular signaling pathway. This pathway regulates muscle contraction by converting neural chemical signals into mechanical responses. In this pathway CHRNA1 interacts closely with proteins such as the acetylcholine ligand and acetylcholine receptor. These interactions ensure effective synaptic transmission at the neuromuscular junction contributing to muscle activation and relaxation cycles.
- Associated diseases and disorders
Nicotinic Acetylcholine Receptor alpha 1 is linked to conditions such as myasthenia gravis and congenital myasthenic syndromes. In myasthenia gravis autoantibodies targeting CHRNA1 hinder normal receptor function on muscles leading to weakness and fatigue. Additionally the protein is associated with congenital myasthenic syndromes where mutations can alter the receptor's function resulting in impaired neuromuscular transmission. The receptor's relationship with other proteins like the acetylcholine receptor and AchR protein plays an important role in these conditions often defining the severity and specific characteristics of the disorders.
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SDS-PAGE - Recombinant Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 protein (Tagged) (ab235797)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab235797.
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