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AB224846

Recombinant Nucleoprotein (His tag)

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Recombinant Nucleoprotein (His tag) is a Lassa virus Josiah Full Length protein, in the 1 to 569 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N, N

1 Images
SDS-PAGE - Recombinant Nucleoprotein (His tag) (AB224846)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Nucleoprotein (His tag) (AB224846)

Discontinuous SDS-PAGE (Tris-Glycine gel)(reduced) analysis of ab224846 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P13699

Animal free

No

Carrier free

No

Species

Lassa virus Josiah

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSASKEIKSFLWTQSLRRELSGYCSNIKLQVVKDAQALLHGLDFSEVSNVQRLMRKERRDDNDLKRLRDLNQAVNNLVELKSTQQKSILRVGTLTSDDLLILAADLEKLKSKVIRTERPLSAGVYMGNLSSQQLDQRRALLNMIGMSGGNQGARAGRDGVVRVWDVKNAELLNNQFGTMPSLTLACLTKQGQVDLNDAVQALTDLGLIYTAKYPNTSDLDRLTQSHPILNMIDTKKSSLNISGYNFSLGAAVKAGACMLDGGNMLETIKVSPQTMDGILKSILKVKKALGMFISDTPGERNPYENILYKICLSGDGWPYIASRTSITGRAWENTVVDLESDGKPQKADSNNSSKSLQSAGFTAGLTYSQLMTLKDAMLQLDPNAKTWMDIEGRPEDPVEIALYQPSSGCYIHFFREPTDLKQFKQDAKYSHGIDVTDLFATQPGLTSAVIDALPRNMVITCQGSDDIRKLLESQGRKDIKLIDIALSKTDSRKYENAVWDQYKDLCHMHTGVVVEKKKRGGKEEITPHCALMDCIMFDAAVSGGLNTSVLRAVLPRDMVFRTSTPRVVL","proteinLength":"Full Length","predictedMolecularWeight":"79 kDa","actualMolecularWeight":null,"aminoAcidEnd":569,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13699","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoprotein also known as NP is a structural protein that plays an important role in the assembly and genomic encapsidation of several viruses including influenza and hantavirus. It has a molecular mass that varies depending on the specific virus but typically ranges from 50 to 60 kDa. Nucleoprotein is expressed predominantly within the nuclear and cytoplasmic regions of infected host cells. It interacts directly with the viral RNA forming a ribonucleoprotein complex that facilitates the viral replication process.
Biological function summary

Nucleoprotein acts as an essential component in the viral lifecycle by aiding in the protection and packaging of viral RNA. It typically associates with other viral proteins to form a ribonucleoprotein complex which is necessary for viral replication and transcription. This complex ensures the encapsidation of the viral genome protecting it from nucleases and allowing proper assembly into new virions. Additionally nucleoprotein has been found to modulate host immune responses potentially aiding viral evasion of host defense mechanisms.

Pathways

Nucleoprotein participates in viral replication and transcription pathways particularly within the context of the host-virus interaction. It is associated with the replication machinery of the virus where it interacts with the polymerase complex which includes proteins such as polymerase basic 1 (PB1) and polymerase basic 2 (PB2). This interaction facilitates the synthesis of complementary RNA strands necessary for viral propagation and the production of viral proteins.

Nucleoprotein’s involvement is significant in conditions like viral hemorrhagic fevers such as those caused by hantavirus which lead to fever and thrombocytopenia. It plays a role in diseases by contributing to viral pathogenesis and influencing disease severity. The interaction of nucleoprotein with other proteins such as glycoproteins found in the viral envelope highlights its importance in the infectious cycle and its potential as a target for therapeutic interventions and vaccine development.
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Specifications

Form

Liquid

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General info

Function

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and thereby participates in the suppression of interferon induction.

Sequence similarities

Belongs to the arenaviridae nucleocapsid protein family.

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Product protocols

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Target data

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and thereby participates in the suppression of interferon induction.
See full target information N
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