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AB226269

Recombinant OmpA protein (Tagged)

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(1 Publication)

Recombinant OmpA protein (Tagged) is a Haemophilus influenzae Full Length protein, in the 22 to 359 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ompP5, ompA, Outer membrane protein P5, OMP P5, Fimbrin, Outer membrane porin A, Outer membrane protein A

3 Images
Mass Spectrometry - Recombinant OmpA protein (Tagged) (AB226269)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant OmpA protein (Tagged) (AB226269)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226269 could indicate that this peptide derived from E.coli-expressed Haemophilus influenzae ompA.

Mass Spectrometry - Recombinant OmpA protein (Tagged) (AB226269)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant OmpA protein (Tagged) (AB226269)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab226269 could indicate that this peptide derived from E.coli-expressed Haemophilus influenzae ompA.

SDS-PAGE - Recombinant OmpA protein (Tagged) (AB226269)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant OmpA protein (Tagged) (AB226269)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226269 with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

10x His tag N-Terminus SUMO tag N-Terminus Myc tag C-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P45996

Animal free

No

Carrier free

No

Species

Haemophilus influenzae

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Sequence info

[{"sequence":"APQENTFYAGVKAGQGSFHDGINNNGAIKKGLSSSNYGYRRNTFTYGVFGGYQILNQDNFGLAAELGYDDFGRAKLREAGKPKAKHTNHGAYLSLKGSYEVLDGLDVYGKAGVALVRSDYKFYEDANGTRDHKKGRHTARASGLFAVGAEYAVLPELAVRLEYQWLTRVGKYRPQDKPNTAINYNPWIGCINAGISYRFGQGEAPVVAAPEMVSKTFSLNSDVTFAFGKANLKPQAQATLDSVYGEISQVKSRKVAVAGYTNRIGSDAFNVKLSQERADSVANYFVAKGVAADAISATGYGEANPVTGATCDQVKGRKALIACLAPDRRVEIAVNGTK","proteinLength":"Full Length","predictedMolecularWeight":"56.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":359,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P45996","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OmpA also known as Outer Membrane Protein A is a significant membrane protein found in many Gram-negative bacteria including Escherichia coli (E. coli). It has a molecular mass of approximately 35 kDa. This protein is expressed in the outer membrane of these bacterial cells. The structure of OmpA includes a beta-barrel that enables it to function as a channel for the passage of small molecules. Its stability and role in maintaining membrane integrity make it an important element of bacterial survival.
Biological function summary

OmpA plays a critical role in interactions between the bacterial cell and its environment. It is involved in cell adhesion and invasion influencing the bacterium's ability to establish infection. This protein does not typically exist as part of a larger complex yet it contributes to the structural and functional roles of the bacterial outer membrane. Furthermore it aids in the stabilization of the outer membrane by interacting with other components including lipopolysaccharides.

Pathways

OmpA is integral to bacterial nutrient uptake mechanisms and the response to environmental changes. It participates in the pathway for porin-mediated transport which includes proteins like FepA another outer membrane protein involved in iron transport. Through its channel-forming properties OmpA influences the diffusion of small molecules and ions underlining its involvement in vital processes of cellular homeostasis and response to oxidative stress.

OmpA is connected to infectious diseases caused by pathogenic E. coli strains and other Gram-negative bacteria. It serves as a virulence factor contributing to pathogenicity by facilitating the invasion of host cells and evading the immune response. Diseases related to these bacteria such as urinary tract infections (UTIs) demonstrate the importance of OmpA in pathogenicity. OmpA also has connections to proteins like TLR2 (Toll-Like Receptor 2) which recognizes bacterial components and activates immune cell responses linking OmpA to inflammation and immune system modulation.

Specifications

Form

Liquid

General info

Function

Acts as a fimbriae subunit, allowing adhesion to host cells.. With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm. Acts as a porin with low permeability that allows slow penetration of small solutes; an internal gate slows down solute passage.

Sequence similarities

Belongs to the outer membrane OOP (TC 1.B.6) superfamily. OmpA family.

Product protocols

Target data

Acts as a fimbriae subunit, allowing adhesion to host cells.. With TolR probably plays a role in maintaining the position of the peptidoglycan cell wall in the periplasm. Acts as a porin with low permeability that allows slow penetration of small solutes; an internal gate slows down solute passage.
See full target information ompA

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biotechnology and applied biochemistry 69:2091-2101 PubMed34664729

2021

Human neutrophil peptide 1 promotes immune sterilization in vivo by reducing the virulence of multidrug-resistant Klebsiella pneumoniae and increasing the ability of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Yun Wang,Xiao-Chun Chen,Zhi-Han Yan,Fan Tu,Tian He,Subash C B Gopinath,Xiao-Hong Rui,Fu-Tao Cao
View all publications

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