Recombinant OV-16 antigen protein (His tag) is a Onchocerca volvulus Full Length protein, in the 17 to 197 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
K I S A E N A N C K K C T P M L V D S A F K E H G I V P D V V S T A P T K L V N V S Y N N L T V N L G N E L T P T Q V K N Q P T K V S W D A E P G A L Y T L V M T D P D A P S R K N P V F R E W H H W L I I N I S G Q N V S S G T V L S D Y I G S G P R K G T G L H R Y V F L V Y K Q P G S I T D T Q H G G N R R N F K V M D F A N K H H L G N P V A G N F F Q A K H E D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Alternative names
OV-16 antigen, OV16
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Recombinant OV-16 antigen protein (His tag) is a Onchocerca volvulus Full Length protein, in the 17 to 197 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P31729-1
- Animal free
No
- Species
Onchocerca volvulus
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
K I S A E N A N C K K C T P M L V D S A F K E H G I V P D V V S T A P T K L V N V S Y N N L T V N L G N E L T P T Q V K N Q P T K V S W D A E P G A L Y T L V M T D P D A P S R K N P V F R E W H H W L I I N I S G Q N V S S G T V L S D Y I G S G P R K G T G L H R Y V F L V Y K Q P G S I T D T Q H G G N R R N F K V M D F A N K H H L G N P V A G N F F Q A K H E D
- Accession
- Protein length
Full Length
- Predicted molecular weight
24 kDa
- Amino acids
17 to 197
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Sequence similarities
Belongs to the phosphatidylethanolamine-binding protein family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The OV-16 antigen also known as Onchocerca volvulus antigen plays an important role in immune response detection. This protein has a mass of about 16 kDa and is explicitly expressed by the filarial helminth Onchocerca volvulus the parasite responsible for onchocerciasis. Researchers commonly study OV-16 because of its immunogenic properties which help in the development of diagnostic tests. OV-16 antigen is not present in humans but is recognized by the host's immune system producing a quantifiable antibody response aiding detection.
- Biological function summary
The host immune system's response to OV-16 antigen involves the specific binding with IgG4 antibodies. The presence of these antibodies indicates exposure to the Onchocerca volvulus infection. OV-16 does not form part of a larger protein complex but serves as a standalone marker to signal infection. By adhering to antibodies in vitro OV-16 helps in the evaluation of filariasis transmission critical for managing and monitoring control programs aiming to eliminate the spread of the disease.
- Pathways
OV-16 antigen connects with immune response pathways that activate the production of specific antibodies. Notably the adaptive immune pathway plays a significant role where IgG4 antibodies get produced as a result of parasite exposure. This specific antibody response serves as a biomarker for diagnosing onchocerciasis therefore OV-16 interacts indirectly with immune signaling proteins responsible for antibody isotype switching during infection.
- Associated diseases and disorders
OV-16 antigen is closely linked to onchocerciasis also called river blindness. This neglected tropical disease leads to visual impairment and skin diseases caused by the parasite Onchocerca volvulus. Through the disease the target associates with other antigens like O. volvulus recombinant antigens important for serological tests. Detecting OV-16-specific antibodies allows health care programs to assess infection rates and effectiveness of treatment initiatives especially in endemic regions.
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1 product image
SDS-PAGE - Recombinant OV-16 antigen protein (His tag) (ab225570)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225570 with 5% enrichment gel and 15% separation gel.
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