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AB239542

Recombinant Pectate lyase 1 protein (Tagged)

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Recombinant Pectate lyase 1 protein (Tagged) is a Hesperocyparis arizonica Full Length protein, in the 22 to 367 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Pectate lyase 1, Major pollen allergen Cup a 1

3 Images
Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab239542 could indicate that this peptide derived from E.coli-expressed Cupressus arizonica (Arizona cypress) (Callitropsis arizonica) Pectate lyase 1.

Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab239542 could indicate that this peptide derived from E.coli-expressed Cupressus arizonica (Arizona cypress) (Callitropsis arizonica) Pectate lyase 1.

SDS-PAGE - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239542.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9SCG9

Animal free

No

Carrier free

No

Species

Hesperocyparis arizonica

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"DNPIDSCWRGDSNWDQNRMKLADCVVGFGSSTMGGKGGEIYTVTSSEDNPVNPTPGTLRYGATREKALWIIFSQNMNIKLQMPLYVAGYKTIDGRGADVHLGNGGPCLFMRKASHVILHGLHIHGCNTSVLGDVLVSESIGVEPVHAQDGDAITMRNVTNAWIDHNSLSDCSDGLIDVTLGSTGITISNNHFFNHHKVMLLGHDDTYDDDKSMKVTVAFNQFGPNAGQRMPRARYGLVHVANNNYDQWNIYAIGGSSNPTILSEGNSFTAPNESYKKEVTKRIGCETTSACANWVWRSTRDAFTNGAYFVSSGKAEDTNIYNSNEAFKVENGNAAPQLTQNAGVVA","proteinLength":"Full Length","predictedMolecularWeight":"42.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":367,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9SCG9","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pectate lyase 1 also known as PL1 or PecL1 is an enzyme that catalyzes the cleavage of pectate a form of pectin into smaller units through a lyase mechanism. The enzyme exhibits a molecular mass of approximately 40 kDa. Pectate lyase 1 is expressed in various plant tissues particularly in areas undergoing cell wall modification such as elongating and ripening fruits leaf abscission zones and germinating seeds. The presence of this enzyme is significant in the breakdown of pectin found in the plant cell wall matrix.
Biological function summary

The action of Pectate lyase 1 aids in cell wall degradation and softening making it essential in the plant's natural growth and development processes. During processes such as fruit ripening leaf fall and seed germination Pectate lyase 1 loosens the cell walls by breaking down pectin which is a significant component of plant cell walls. Pectate lyase 1 operates in coordination with other cell wall-degrading enzymes such as polygalacturonase within a complex that works synergistically to facilitate plant tissue differentiation and development.

Pathways

The activity of Pectate lyase 1 fits into the cell wall modification and glycosaminoglycan degradation pathways. In the cell wall modification pathway Pectate lyase 1 interacts with proteins like polygalacturonase to dismantle pectate contributing to cell separation and expansion. Additionally the enzyme participates in the glycosaminoglycan degradation pathway highlighting its involvement in polysaccharide breakdown alongside other enzymes ensuring the dynamic nature of plant cell walls to accommodate growth and environmental adaptation.

Disruptions in the expression or activity of Pectate lyase 1 can impact both agricultural and environmental contexts. One such impact is fruit softening where overactivity of this enzyme leads to excessive softening and post-harvest losses. Another disease associated with Pectate lyase 1 is bacterial soft rot where pathogens exploit the enzymatic action of Pectate lyase 1 to invade plant tissues. In these contexts the role of other associated proteins such as cellulase becomes important as they work in tandem with Pectate lyase 1 to accelerate tissue degradation during plant pathogenesis.
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Specifications

Form

Liquid

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General info

Function

Has pectate lyase activity.

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

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Product protocols

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Target data

Has pectate lyase activity.
See full target information Pectate lyase 1
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