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Recombinant Phosphinotricin Acetyl Transferase protein (His tag) is a Streptomyces hygroscopicus Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M S P E R R P A D I R R A T E A D M P A V C T I V N H Y I E T S T V N F R T E P Q E P Q E W T D D L V R L R E R Y P W L V A E V D G E V A G I A Y A G P W K A R N A Y D W T A E S T V Y V S P R H Q R T G L G S T L Y T H L L K S L E A Q G F K S V V A V I G L P N D P S V R M H E A L G Y A P R G M L R A A G F K H G N W H D V G F W Q L D F S L P V P P R P V L P V T E I

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.

Targets

Phosphinotricin Acetyl Transferase

Alternative names

Recommended products

Recombinant Phosphinotricin Acetyl Transferase protein (His tag) is a Streptomyces hygroscopicus Full Length protein, in the 1 to 183 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
P16426-1
Animal free

No

Species

Streptomyces hygroscopicus

Concentration
Loading...
Storage buffer

pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

Sequence info

Amino acid sequence

M S P E R R P A D I R R A T E A D M P A V C T I V N H Y I E T S T V N F R T E P Q E P Q E W T D D L V R L R E R Y P W L V A E V D G E V A G I A Y A G P W K A R N A Y D W T A E S T V Y V S P R H Q R T G L G S T L Y T H L L K S L E A Q G F K S V V A V I G L P N D P S V R M H E A L G Y A P R G M L R A A G F K H G N W H D V G F W Q L D F S L P V P P R P V L P V T E I

Accession

P16426

Protein length

Full Length

Predicted molecular weight

24.6 kDa

Amino acids

1 to 183

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

General info

Function

Inactivates phosphinothricin (PPT) by transfer of an acetyl group from acetyl CoA. Can also acetylate demethylphosphinothricin but not PTT or glutamate. This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance.

Sequence similarities

Belongs to the acetyltransferase family. PAT/BAR subfamily.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Phosphinotricin Acetyl Transferase also known as PAT serves a mechanical role in the inactivation of phosphinothricin an active component in some herbicides. PAT catalyzes the transfer of an acetyl group from acetyl-CoA to phosphinothricin effectively neutralizing it. The approximate mass of PAT is 21 kDa. It is often expressed in genetically modified crops enabling them to tolerate certain herbicides that include phosphinothricin as an active ingredient.

Biological function summary

Phosphinotricin acetyl transferase contributes to herbicide resistance. It ensures the detoxification process allowing plants to grow even in the presence of herbicides that inhibit glutamine synthetase. PAT is not typically known to form part of a larger complex like some other enzymes but its activity is important for maintaining plant viability under herbicide pressure. The expression of this enzyme in plants is a form of an inducible stress response to environmental changes.

Pathways

Phosphinotricin acetyl transferase plays an important role in the metabolic pathway for xenobiotic detoxification. This enzyme is pivotal in the acetylation pathway aiding in counteracting herbicide action. PAT works alongside other metabolic enzymes facilitating detoxification although it is not directly interlinked with common metabolic proteins its function highlights the synergistic nature of plant metabolic systems when dealing with external chemical stresses.

Associated diseases and disorders

Phosphinotricin acetyl transferase's application relates to agricultural practices rather than direct human diseases although indirectly it could affect human health due to its role in food crop production. Increased use of PAT-expressing crops might influence patterns of herbicide use with implications for biodiversity and ecosystem health. No direct connections to specific human-related protein interactions exist as PAT functions and impacts are largely associated with plant biology and crop engineering rather than mammalian systems.

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