Recombinant Pig EPO protein (His tag) is a Pig Full Length protein, in the 27 to 194 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A P P R L I C D S R V L E R Y I L E A K E G E N A T M G C A E S C S F S E N I T V P D T K V N F Y A W K R M E V Q Q Q A M E V W Q G L A L L S E A I L Q G Q A L L A N S S Q P S E A L Q L H V D K A V S G L R S L T S L L R A L G A Q K E A I P L P D A S P S S A T P L R T F A V D T L C K L F R N Y S N F L R G K L T L Y T G E A C R R R D R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Additional Targets
EPO
Alternative names
Erythropoietin, EPO
Recommended products
Recombinant Pig EPO protein (His tag) is a Pig Full Length protein, in the 27 to 194 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P49157-1
- Animal free
- No
- Species
- Pig
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS, 6% Trehalose
Sequence info
Amino acid sequence
- A P P R L I C D S R V L E R Y I L E A K E G E N A T M G C A E S C S F S E N I T V P D T K V N F Y A W K R M E V Q Q Q A M E V W Q G L A L L S E A I L Q G Q A L L A N S S Q P S E A L Q L H V D K A V S G L R S L T S L L R A L G A Q K E A I P L P D A S P S S A T P L R T F A V D T L C K L F R N Y S N F L R G K L T L Y T G E A C R R R D R
- Accession
- P49157
- Protein length
- Full Length
- Predicted molecular weight
- 21 kDa
- Amino acids
- 27 to 194
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
nan
General info
- Function
Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
- Sequence similarities
Belongs to the EPO/TPO family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Erythropoietin often called EPO or erythropoietin protein is a glycoprotein hormone with a mass of approximately 30.4 kDa. It plays a mechanical role in the production of red blood cells (erythropoiesis) by acting as a signaling molecule that binds the erythropoietin receptor on erythroid progenitor cells in the bone marrow. Erythropoietin is mainly expressed in the kidneys with some expression in the liver. It controls the proliferation and differentiation of precursor cells into mature red blood cells which are responsible for oxygen transport.
- Biological function summary
Erythropoietin regulates blood oxygen levels by stimulating erythrocyte production. It is not part of a larger complex but interacts directly with the erythropoietin receptor activating intracellular pathways necessary for erythroid progenitor cell survival and proliferation. Eyrthropoietin production increases in response to low oxygen levels in tissues ensuring adequate oxygen delivery throughout the body by increasing red blood cell count.
- Pathways
Erythropoietin predominantly influences the JAK-STAT signaling pathway. Upon binding to its receptor on erythroid progenitors it activates this pathway leading to gene expression changes that promote survival and proliferation of these cells. This pathway is also involved in various immune responses and cell growth processes. Erythropoietin signaling has a regulatory relationship with other growth factors like thrombopoietin which is another hematopoietic hormone.
- Associated diseases and disorders
Erythropoietin is important in the treatment of anemia especially anemia associated with chronic kidney disease where natural EPO production decreases. Abnormal levels of erythropoietin are associated with conditions such as polycythemia vera a disorder characterized by excessive red blood cell production. EPO levels can be affected by production defects in the kidneys linking it to proteins like the von Hippel-Lindau tumor suppressor which regulates erythropoietin transcription under low oxygen conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Pig EPO protein (His tag) (ab267862)
SDS-PAGE analysis of recombinant pig EPO protein (His tag) (ab267862)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com