Recombinant Pig GPX5 protein (Tagged) is a Pig Full Length protein, in the 22 to 219 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Yeast
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
N S N L E K M D C Y K D V T G T I Y D Y D A F T L N G N E H I Q F K Q Y A G K H V L F V N V A T Y C G L T A Q Y P E L N T L Q E E L K P F G L V V L G F P C N Q F G K Q E P G E N S E I L L G L K Y V R P G G G Y V P N F Q L F E K G D V N G E K E Q K V F T F L K H S C P H P S E L I G S I G Y I S W E P I R V H D I R W N F E K F L V G P D G V P V M R W V H E T P I S T V K S D I L A Y L K Q F K T E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction.
Alternative names
Epididymal secretory glutathione peroxidase, Epididymis-specific glutathione peroxidase-like protein, Glutathione peroxidase 5, EGLP, GPx-5, GSHPx-5, GPX5
Recommended products
Recombinant Pig GPX5 protein (Tagged) is a Pig Full Length protein, in the 22 to 219 aa range, expressed in Yeast, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Yeast
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- O18994-1
- Animal free
No
- Species
Pig
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
N S N L E K M D C Y K D V T G T I Y D Y D A F T L N G N E H I Q F K Q Y A G K H V L F V N V A T Y C G L T A Q Y P E L N T L Q E E L K P F G L V V L G F P C N Q F G K Q E P G E N S E I L L G L K Y V R P G G G Y V P N F Q L F E K G D V N G E K E Q K V F T F L K H S C P H P S E L I G S I G Y I S W E P I R V H D I R W N F E K F L V G P D G V P V M R W V H E T P I S T V K S D I L A Y L K Q F K T E
- Accession
- Protein length
Full Length
- Predicted molecular weight
26.1 kDa
- Amino acids
22 to 219
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction.
- Sequence similarities
Belongs to the glutathione peroxidase family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GPX5 also known as Glutathione Peroxidase 5 or Epididymal Secretory Glutathione Peroxidase is an antioxidant enzyme that reduces hydrogen peroxide and lipid peroxides. It belongs to the glutathione peroxidase family and has a mass of approximately 22 kDa. GPX5 is expressed mainly in the epididymis of the male reproductive tract. It plays an important role in protecting sperm from oxidative stress by neutralizing harmful peroxides which can damage cellular structures and affect sperm functionality.
- Biological function summary
GPX5 functions to preserve the integrity of sperm cells during their maturation. By reducing oxidative stress it safeguards the membrane lipid components and essential proteins from oxidative damage. GPX5 operates independently and does not require selenium as a cofactor which differentiates it from other family members. This unique characteristic allows it to function in environments that may have varying selenium levels providing an essential antioxidant defense in sperm-rich regions.
- Pathways
GPX5 integrates into cellular antioxidant mechanisms and is involved in the reduction-oxidation (redox) homeostasis. It participates in the detoxification of peroxides alongside proteins such as catalase and other glutathione peroxidases including GPX1. The pathway interaction establishes a balance between oxidants and antioxidants maintaining cellular health and efficiency in detoxifying reactive oxygen species.
- Associated diseases and disorders
GPX5 potentially associates with male infertility due to its role in safeguarding sperm from oxidative damage. Lower activity or expression levels of GPX5 may contribute to increased oxidative stress in spermatozoa leading to impaired sperm function and reduced fertility. Additionally oxidative stress-related conditions could be linked to other proteins such as superoxide dismutases which like GPX5 play roles in reducing reactive oxygen species within reproductive tissues.
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1 product image
SDS-PAGE - Recombinant Pig GPX5 protein (Tagged) (ab225649)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225649 with 5% enrichment gel and 15% separation gel.
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