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AB233686

Recombinant pig IL-4 protein (Active)

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Recombinant pig IL-4 protein (Active) is a Pig Full Length protein, in the 25 to 133 aa range, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1, IL4

2 Images
Functional Studies - Recombinant pig IL-4 protein (Active) (AB233686)
  • FuncS

Supplier Data

Functional Studies - Recombinant pig IL-4 protein (Active) (AB233686)

Pig IL4 induced proliferation of TF-1 cells. ED50 ≤ 2 ng/mL (≥ 5.0 x 10^5 units/mg).

SDS-PAGE - Recombinant pig IL-4 protein (Active) (AB233686)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant pig IL-4 protein (Active) (AB233686)

SDS-PAGE - Recombinant pig IL4 protein (Active) (ab233686) at 1 μg.
Lane 1 : Non-reducing conditions.
Lane 2 : Reducing conditions.
4-20% Tris-Glycine gel stained with Coomassie Blue.
Expected MW 12.7 kDa.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

TF-1 cell proliferation: ED50 ≤ 2 ng/mL (≥ 5.0 x 105 units/mg).

Accession

Animal free

No

Carrier free

Yes

Species

Pig

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MHKCDITLQEIIKTLNILTARKNSCMELPVTDVFAAPENTTEKETFCRASTVLRHIYRHHTCMKSLLSGLDRNLSSMANMTCSVHEAKKSTLKDFLERLKTIMKEKYSKC","proteinLength":"Full Length","predictedMolecularWeight":"12.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":133,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q04745","tags":[]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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General info

Function

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

Sequence similarities

Belongs to the IL-4/IL-13 family.

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Product protocols

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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information IL4
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Product promise

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