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AB240861

Recombinant Pneumolysin protein (His tag)

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(1 Publication)

Recombinant Pneumolysin protein (His tag) is a Full Length protein, in the 2 to 471 aa range, expressed in Yeast, with >90%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Pneumolysin protein (His tag) (AB240861)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Pneumolysin protein (His tag) (AB240861)

Analysis of ab240861 by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Key facts

Purity

>90% SDS-PAGE

Expression system

Yeast

Tags

6x His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Animal free

No

Carrier free

No

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND","proteinLength":"Full Length","predictedMolecularWeight":"54.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":471,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":null,"tags":[{"tag":"6x His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pneumolysin also known as Ply or ply-004 is a toxin protein produced by Streptococcus pneumoniae a bacterium responsible for many infections. It has a molecular mass of approximately 53 kDa. Pneumolysin is a cholesterol-dependent cytolysin meaning that it targets and binds to cholesterol in host cell membranes leading to pore formation and cell lysis. The toxin is predominantly expressed in S. pneumoniae where it plays an essential role in pathogenicity.
Biological function summary

Pneumolysin affects host cells by disrupting cellular integrity and immune responses. It is not part of a larger protein complex. Pneumolysin can inhibit cilia movement in the respiratory tract impairing the host’s ability to clear mucus and pathogens. This toxin also activates the host immune system by promoting the release of pro-inflammatory cytokines which can result in tissue damage.

Pathways

Pneumolysin plays a significant role in the inflammatory response and cell apoptosis pathways. Within the inflammatory pathway it can trigger the host's immune cells to release cytokines such as interleukin-6 and tumor necrosis factor-alpha. This response leads to increased vascular permeability and leukocyte migration. Pneumolysin-mediated cell lysis also contributes to apoptosis impacting cell survival. The toxin's action relates it to proteins involved in the immune signaling cascades.

Pneumolysin plays a vital role in diseases such as pneumonia and meningitis both caused by S. pneumoniae. The disruption of host cell membranes and induction of inflammation contribute directly to disease symptoms. Pneumolysin interacts with proteins involved in the host's immune defense during infection. These interactions and its cytotoxic properties make pneumolysin a target for research into vaccine and therapeutic strategies against pneumococcal diseases.
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Specifications

Form

Liquid

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General info

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Product protocols

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Target data

See full target information Pneumolysin

Additional targets

Pneumolysin
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e38611 PubMed39397991

2024

Glycyrrhetinic acid reduces lung inflammation caused by pneumococcal infection by reducing the toxicity of pneumolysin.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Xu,Ying Ding,Hongji Wu,Donglin Li,Yudi Li,Yibo Hu,Haoji Meng
View all publications
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