Recombinant Pneumolysin protein (Tagged) is a Full Length protein, in the 2 to 471 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
A N K A V N D F I L A M N Y D K K K L L T H Q G E S I E N R F I K E G N Q L P D E F V V I E R K K R S L S T N T S D I S V T A T N D S R L Y P G A L L V V D E T L L E N N P T L L A V D R A P M T Y S I D L P G L A S S D S F L Q V E D P S N S S V R G A V N D L L A K W H Q D Y G Q V N N V P A R M Q Y E K I T A H S M E Q L K V K F G S D F E K T G N S L D I D F N S V H S G E K Q I Q I V N F K Q I Y Y T V S V D A V K N P G D V F Q D T V T V E D L K Q R G I S A E R P L V Y I S S V A Y G R Q V Y L K L E T T S K S D E V E A A F E A L I K G V K V A P Q T E W K Q I L D N T E V K A V I L G G D P S S G A R V V T G K V D M V E D L I Q E G S R F T A D H P G L P I S Y T T S F L R D N V V A T F Q N S T D Y V E T K V T A Y R N G D L L L D H S G A Y V A Q Y Y I T W N E L S Y D H Q G K E V L T P K A W D R N G Q D L T A H F T T S I P L K G N V R N L S V K I R E C T G L A W E W W R T V Y E K T D L P L V R K R T I S I W G T T L Y P Q V E D K V E N D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Additional Targets
Pneumolysin
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Recombinant Pneumolysin protein (Tagged) is a Full Length protein, in the 2 to 471 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Animal free
- No
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- A N K A V N D F I L A M N Y D K K K L L T H Q G E S I E N R F I K E G N Q L P D E F V V I E R K K R S L S T N T S D I S V T A T N D S R L Y P G A L L V V D E T L L E N N P T L L A V D R A P M T Y S I D L P G L A S S D S F L Q V E D P S N S S V R G A V N D L L A K W H Q D Y G Q V N N V P A R M Q Y E K I T A H S M E Q L K V K F G S D F E K T G N S L D I D F N S V H S G E K Q I Q I V N F K Q I Y Y T V S V D A V K N P G D V F Q D T V T V E D L K Q R G I S A E R P L V Y I S S V A Y G R Q V Y L K L E T T S K S D E V E A A F E A L I K G V K V A P Q T E W K Q I L D N T E V K A V I L G G D P S S G A R V V T G K V D M V E D L I Q E G S R F T A D H P G L P I S Y T T S F L R D N V V A T F Q N S T D Y V E T K V T A Y R N G D L L L D H S G A Y V A Q Y Y I T W N E L S Y D H Q G K E V L T P K A W D R N G Q D L T A H F T T S I P L K G N V R N L S V K I R E C T G L A W E W W R T V Y E K T D L P L V R K R T I S I W G T T L Y P Q V E D K V E N D
- Protein length
- Full Length
- Predicted molecular weight
- 68.8 kDa
- Amino acids
- 2 to 471
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pneumolysin also known as Ply or ply-004 is a toxin protein produced by Streptococcus pneumoniae a bacterium responsible for many infections. It has a molecular mass of approximately 53 kDa. Pneumolysin is a cholesterol-dependent cytolysin meaning that it targets and binds to cholesterol in host cell membranes leading to pore formation and cell lysis. The toxin is predominantly expressed in S. pneumoniae where it plays an essential role in pathogenicity.
- Biological function summary
Pneumolysin affects host cells by disrupting cellular integrity and immune responses. It is not part of a larger protein complex. Pneumolysin can inhibit cilia movement in the respiratory tract impairing the host’s ability to clear mucus and pathogens. This toxin also activates the host immune system by promoting the release of pro-inflammatory cytokines which can result in tissue damage.
- Pathways
Pneumolysin plays a significant role in the inflammatory response and cell apoptosis pathways. Within the inflammatory pathway it can trigger the host's immune cells to release cytokines such as interleukin-6 and tumor necrosis factor-alpha. This response leads to increased vascular permeability and leukocyte migration. Pneumolysin-mediated cell lysis also contributes to apoptosis impacting cell survival. The toxin's action relates it to proteins involved in the immune signaling cascades.
- Associated diseases and disorders
Pneumolysin plays a vital role in diseases such as pneumonia and meningitis both caused by S. pneumoniae. The disruption of host cell membranes and induction of inflammation contribute directly to disease symptoms. Pneumolysin interacts with proteins involved in the host's immune defense during infection. These interactions and its cytotoxic properties make pneumolysin a target for research into vaccine and therapeutic strategies against pneumococcal diseases.
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1 product image
SDS-PAGE - Recombinant Pneumolysin protein (Tagged) (ab236193)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236193.
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