Recombinant Protein A + Protein G
Be the first to review this product! Submit a review
|
(1 Publication)
Recombinant Protein A + Protein G is a Staphylococcus aureus subsp. aureus NCTC 8325 Fragment protein, expressed in Escherichia coli, with >98%, suitable for SDS-PAGE, AP.
View Alternative Names
SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA, Immunoglobulin G-binding protein G, IgG-binding protein G, spg, IgG-binding protein G, Immunoglobulin G binding protein G [Precursor], SPG2_STRSG, spg, spg immunoglobulin G-binding protein
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Protein A + Protein G (AB52213)
4-20% SDS-PAGE of Protein A/G : 10, 15 and 20 µg of Recombinant Protein A/G loaded in each lane under reducing conditions and stained with Coomassie Blue. Recombinant Protein A/G has a predicted MW of ∼ 59.7kDa.
Complementary Products
Reagents, Controls & Accessories
AB313996
Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)
- 0
- 0
Reactivity data
Product details
This product is manufactured by BioVision, an Abcam company and was previously called 6502 Protein A/G. 6502-1 is the same size as the 1 mg size of ab52213.
This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.
Protein A+G binds to all IgG subclasses (from various mammalian species).
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Lyophilized
General info
Function
Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
Sequence similarities
Belongs to the immunoglobulin-binding protein SpA family.
Target data
Additional targets
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Journal of chemical neuroanatomy 39:151-65 PubMed20043984
2010
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com