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AB7460

Recombinant Protein G (HRP)

5

(1 Review)

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(13 Publications)

Recombinant Protein G (HRP) is a Streptococcus sp. 'group G' Fragment protein, in the 190 to 384 aa range, expressed in Escherichia coli, with >98%, suitable for Dot, WB, ELISA, IHC-P.

View Alternative Names

Immunoglobulin G-binding protein G, IgG-binding protein G, spg

2 Images
Western blot - Recombinant Protein G (HRP) (AB7460)
  • WB

AbReview23213****

Western blot - Recombinant Protein G (HRP) (AB7460)

Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.

All lanes:

Western blot - Recombinant Protein G (HRP) (ab7460)

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Image courtesy of an anonymous Abreview.

Dot Blot - Recombinant Protein G (HRP) (AB7460)
  • Dot

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Dot Blot - Recombinant Protein G (HRP) (AB7460)

Dot Blot analysis of ab7460 Protein G protein (HRP).

Load : 200ng of ab7460 followed by a 3-fold serial dilution

Antigens :
Row 1 : Human IgG
Row 2 : Rat IgG
Row 3 : Dog IgG

Primary antibody : none
Secondary antibody : Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature

Block : Blocking buffer for 60 min at room temperature

Key facts

Purity

>98%

Expression system

Escherichia coli

Tags

Tag free

Applications

IHC-P, Dot, ELISA, WB

applications

Biologically active

No

Conjugation

HRP

Excitation/Emission
Accession

P19909

Animal free

No

Carrier free

No

Species

Streptococcus sp. 'group G'

Reconstitution

reconstitute with water at 1mL

Storage buffer

pH: 7.2 Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/40000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/200000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" } } }
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Product details

No reaction was observed against anti-Protein A.
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Sequence info

[{"sequence":"LDKYGVSDYHKNLINNAKTVEGVKDLQAQVVESAKKARISEATDGLSDFLKSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYYKNLINNAKTVEGVKALIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLK","proteinLength":"Fragment","predictedMolecularWeight":"63 kDa","actualMolecularWeight":null,"aminoAcidEnd":384,"aminoAcidStart":190,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P19909","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein G also known as IgG-binding protein is a cell wall protein from Streptococcus species. This protein weighs approximately 22 kDa which you might also know as protein G molecular weight (protein G MW). Protein G size allows it to bind specifically to immunoglobulin G (IgG) and other subclasses. Researchers often clone and express protein G recombinantly resulting in wide availability of recombinant protein G. Its expression primarily occurs in bacterial cell cultures for easy purification and use in laboratory research.
Biological function summary

Protein G plays a role in immune evasion by binding to the Fc region of IgG antibodies. This binding limits the immune system's ability to clear bacterial infections by preventing antibody-mediated opsonization and phagocytosis. Protein G can also form part of a larger immobilization complex in various experimental setups aiding in the purification and detection of antibodies. Protein G HRP conjugates further extend its utility in assay systems by enabling colorimetric detection.

Pathways

The interaction between protein G and IgG significantly impacts the immune response pathways. The binding of protein G to the Fc region of IgG interferes with pathways involving IgG-mediated activation of Fc receptors on immune cells. This affects signaling pathways like ADCC (antibody-dependent cellular cytotoxicity) and phagocytosis. Protein A is another bacterial protein that binds IgG similarly though it differs in affinity and subclass specificity.

Protein G's interaction with IgG relates to conditions involving bacterial infections and immune system evasion mechanisms. It impacts conditions like bacterial endocarditis by providing bacteria a means to escape immune detection. By binding IgG and interfering with the normal immune function protein G might indirectly affect proteins such as complement components. These interactions underline the importance of understanding protein G's function in designing therapeutic interventions in bacterial infections.
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Specifications

Form

Lyophilized

Additional notes

Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G.

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General info

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Product protocols

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Target data

See full target information spg

Additional targets

Protein G
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 15: PubMed40003558

2025

DYRK1A Up-Regulation Specifically Impairs a Presynaptic Form of Long-Term Potentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Aude-Marie Lepagnol-Bestel,Simon Haziza,Julia Viard,Paul A Salin,Arnaud Duchon,Yann Herault,Michel Simonneau

Pathogens (Basel, Switzerland) 13: PubMed39599470

2024

Serological Evidence of Potential Marburg Virus Circulation in Livestock and Dogs in Ghana.

Applications

Unspecified application

Species

Unspecified reactive species

Theophilus Odoom,Sherry Ama Mawuko Johnson,William Tasiame,Meyir Y Ziekah,Joseph K Abuh,Benita Anderson,Fenteng Danso,Richard K Abbiw,Franklin Y Nuokpem,Emmanuel Allegye-Cudjoe,Charles Lewis,Bonto Faburay

Nature communications 14:6208 PubMed37798264

2023

Acetylation-dependent coupling between G6PD activity and apoptotic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wu,Natali H Muskat,Inbar Dvilansky,Omri Koren,Anat Shahar,Roi Gazit,Natalie Elia,Eyal Arbely

Life science alliance 5: PubMed35914814

2022

Chr21 protein-protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Viard,Yann Loe-Mie,Rachel Daudin,Malik Khelfaoui,Christine Plancon,Anne Boland,Francisco Tejedor,Richard L Huganir,Eunjoon Kim,Makoto Kinoshita,Guofa Liu,Volker Haucke,Thomas Moncion,Eugene Yu,Valérie Hindie,Henri Bléhaut,Clotilde Mircher,Yann Herault,Jean-François Deleuze,Jean-Christophe Rain,Michel Simonneau,Aude-Marie Lepagnol-Bestel

Autophagy 19:204-223 PubMed35506243

2022

Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Juhyung Lee,Yue Xu,Layla Saidi,Miao Xu,Konrad Zinsmaier,Yihong Ye

Cell reports 38:110611 PubMed35354052

2022

Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans.

Applications

Unspecified application

Species

Unspecified reactive species

Isabella Huettner,Stefanie A Krumm,Sonia Serna,Katarzyna Brzezicka,Serena Monaco,Samuel Walpole,Angela van Diepen,Fiona Allan,Thomas Hicks,Simon Kimuda,Aidan M Emery,Elise Landais,Cornelis H Hokke,Jesus Angulo,Niels Reichardt,Katie J Doores

Frontiers in immunology 13:833515 PubMed35309340

2022

Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lam Ng,Xiaohui Wang,Chuanbin Yang,Chengfu Su,Min Li,Allen Ka Loon Cheung

Journal of dairy science 104:10111-10120 PubMed34127267

2021

Evaluation of test characteristics of 2 ELISA tests applied to bulk tank milk and claw-trimming records for herd-level diagnosis of bovine digital dermatitis using latent class analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ingrid H Holmøy,Lina Ahlén,Jenny Frössling,Liv Sølverød,Menno Holzhauer,Ane Nødtvedt,Terje Fjeldaas

Molecular therapy. Methods & clinical development 10:105-112 PubMed30073181

2018

Prevalence of Pre-existing Antibodies to CRISPR-Associated Nuclease Cas9 in the USA Population.

Applications

Unspecified application

Species

Unspecified reactive species

Vijaya L Simhadri,Joseph McGill,Shane McMahon,Junxia Wang,Haiyan Jiang,Zuben E Sauna

PloS one 12:e0185495 PubMed28957443

2017

A glycosylated recombinant subunit candidate vaccine consisting of Ehrlichia ruminantium major antigenic protein1 induces specific humoral and Th1 type cell responses in sheep.

Applications

ELISA

Species

Unspecified reactive species

Bonto Faburay,Jodi McGill,Frans Jongejan
View all publications
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