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AB236164

Recombinant Pseudomonas aeruginosa ompF protein (His tag)

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Recombinant Pseudomonas aeruginosa ompF protein (His tag) is a Pseudomonas aeruginosa PAO1 Full Length protein, in the 25 to 350 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

PA1777, oprF, Outer membrane porin F, Putative outer membrane porin F protein, YE1563, orpF

1 Images
SDS-PAGE - Recombinant Pseudomonas aeruginosa ompF protein (His tag) (AB236164)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Pseudomonas aeruginosa ompF protein (His tag) (AB236164)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel analysis of ab236164.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P13794

Animal free

No

Carrier free

No

Species

Pseudomonas aeruginosa PAO1

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QGQNSVEIEAFGKRYFTDSVRNMKNADLYGGSIGYFLTDDVELALSYGEYHDVRGTYETGNKKVHGNLTSLDAIYHFGTPGVGLRPYVSAGLAHQNITNINSDSQGRQQMTMANIGAGLKYYFTENFFAKASLDGQYGLEKRDNGHQGEWMAGLGVGFNFGGSKAAPAPEPVADVCSDSDNDGVCDNVDKCPDTPANVTVDANGCPAVAEVVRVQLDVKFDFDKSKVKENSYADIKNLADFMKQYPSTSTTVEGHTDSVGTDAYNQKLSERRANAVRDVLVNEYGVEGGRVNAVGYGESRPVADNATAEGRAINRRVEAEVEAEAK","proteinLength":"Full Length","predictedMolecularWeight":"39.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":350,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P13794","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ompF protein known as Outer membrane protein F serves as a porin in the outer membrane of Escherichia coli. It facilitates the passive diffusion of small molecules across the cell membrane. This protein has a trimeric structure where each monomer forms a beta-barrel. Each of the monomers has a mass of about 38 kDa. The ompF protein is typically expressed in response to varying environmental factors like osmolarity and is important for regulating molecule entry into the bacterial cell.
Biological function summary

OmpF plays a significant role in maintaining homeostasis and nutrient uptake in bacteria. It forms part of the beta-barrel assembly machinery complex which handles the insertion and folding of beta-barrel proteins into the outer membrane. This protein adjusts bacteria's permeability properties ensuring necessary ions and small nutrients reach the cell’s interior while controlling the access of harmful substances.

Pathways

OmpF participates in nutrient uptake and ion transport pathways significantly affecting bacterial survival and adaptation processes. OmpF interacts with other proteins in the porin family such as ompC which collectively regulate ion passage and osmotic balance within the bacterial cell envelope. These actions occur in pathways related to membrane permeability which are vital for bacteria responding to environmental stress.

OmpF has a linkage to antibiotic resistance mechanisms in pathogenic bacteria. Alterations in the ompF protein or expression can reduce antibiotic susceptibility complicating treatment of infections caused by Escherichia coli. This protein has connections with various resistance proteins where modulating such pathways may enhance antibiotic effectiveness. Understanding its role and interactions can guide strategies to counteract antimicrobial resistance in clinical settings.
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Specifications

Form

Liquid

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General info

Function

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.

Sequence similarities

Belongs to the outer membrane OOP (TC 1.B.6) superfamily.

Post-translational modifications

Might contain two disulfide bonds.

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Product protocols

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Target data

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
See full target information oprF

Additional targets

ompF
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