Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag) is a Pseudomonas aeruginosa PAO1 Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
Amino acid sequence
M R N D G G F L L W W D G L R S E M Q P I H D S Q G V F A V L E K E V R R L G F D Y Y A Y G V R H T I P F T R P K T E V H G T Y P K A W L E R Y Q M Q N Y G A V D P A I L N G L R S S E M V V W S D S L F D Q S R M L W N E A R D W G L C V G A T L P I R A P N N L L S V L S V A R D Q Q N I S S F E R E E I R L R L R C M I E L L T Q K L T D L E H P M L M S N P V C L S H R E R E I L Q W T A D G K S S G E I A I I L S I S E S T V N F H H K N I Q K K F D A P N K T L A A A Y A A A L G L I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). Binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone).
Targets
Regulatory protein RhlR
Alternative names
Regulatory protein RhlR, Elastase modulator, lasM, vsmR, PA3477, rhlR
Recommended products
Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag) is a Pseudomonas aeruginosa PAO1 Full Length protein, in the 1 to 241 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE, MS
- Biologically active
No
- Accession
- P54292-1
- Animal free
No
- Species
Pseudomonas aeruginosa PAO1
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M R N D G G F L L W W D G L R S E M Q P I H D S Q G V F A V L E K E V R R L G F D Y Y A Y G V R H T I P F T R P K T E V H G T Y P K A W L E R Y Q M Q N Y G A V D P A I L N G L R S S E M V V W S D S L F D Q S R M L W N E A R D W G L C V G A T L P I R A P N N L L S V L S V A R D Q Q N I S S F E R E E I R L R L R C M I E L L T Q K L T D L E H P M L M S N P V C L S H R E R E I L Q W T A D G K S S G E I A I I L S I S E S T V N F H H K N I Q K K F D A P N K T L A A A Y A A A L G L I
- Accession
- Protein length
Full Length
- Predicted molecular weight
47.6 kDa
- Amino acids
1 to 241
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). Binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone).
- Sequence similarities
Belongs to the autoinducer-regulated transcriptional regulatory protein family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Regulatory protein RhlR also known as RhlR transcriptional regulator plays an important role in the quorum sensing system of Pseudomonas aeruginosa. It acts mechanically as a transcription factor by binding to specific DNA sequences regulating gene expression linked to virulence factors. RhlR has a molecular mass of around 27 kDa. This protein is expressed within the bacterial cells where it forms part of a communication system that responds to population density.
- Biological function summary
The regulatory protein RhlR controls the transcription of genes involved in the production of rhamnolipids pyocyanin and elastase which contribute to bacterial virulence. RhlR forms a part of the complex interplay between LasR and RhlI which comprise the quorum sensing system. This complex communication network allows the bacteria to modulate its pathogenic behavior and adapt to different environments or host conditions.
- Pathways
The regulatory protein RhlR serves a primary function within the quorum sensing pathway of Pseudomonas aeruginosa. It interacts significantly with another quorum sensing protein LasR to regulate a hierarchy of genes responsible for pathogenicity. The pathways involving RhlR are critical for the coordinated expression of virulence factors contributing to biofilm formation and increased bacterial resistance to antibiotics.
- Associated diseases and disorders
Regulatory protein RhlR contributes to the pathogenic profile of Pseudomonas aeruginosa in infections like cystic fibrosis and burn wound infections. The enhanced virulence mediated by RhlR impacts the severity and persistence of these infections. RhlR is intricately connected with proteins like LasR through these diseases as they work together in the quorum sensing pathway to promote the bacterium’s survival and adaptation in hostile environments.
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3 product images
SDS-PAGE - Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag) (ab225569)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225569 with 5% enrichment gel and 15% separation gel.
Mass Spectrometry - Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag) (ab225569)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab225569 could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Regulatory protein RhlR.
Mass Spectrometry - Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (His tag) (ab225569)
Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS analysis result of ab225569 could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) Regulatory protein RhlR.
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