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AB271726

Recombinant Rabbit Angiopoietin 2/ANG2 protein (His tag C-Terminus)

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Recombinant Rabbit Angiopoietin 2/ANG2 protein (His tag C-Terminus) is a Fragment protein, in the 19 to 496 aa range, expressed in HEK 293 cells, with >70%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Rabbit Angiopoietin 2/ANG2 protein (His tag C-Terminus) (AB271726)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Rabbit Angiopoietin 2/ANG2 protein (His tag C-Terminus) (AB271726)

SDS-PAGE analysis of 3 μg ab271726.

This protein runs at an apparent higher MW on SDS PAGE due to glycosylation.

Key facts

Purity

>70% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Animal free

No

Carrier free

No

Storage buffer

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB69492

Recombinant human Angiopoietin 1 protein

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Assay Kits

AB316914

Human Angiopoietin 2/ANG2 ELISA Kit

SimpleStep

Sandwich ELISA - Human Angiopoietin 2/ANG2 ELISA Kit (AB316914)

  • 0
  • 0
Primary Antibodies

AB155106

Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Angiopoietin 2/ANG2 antibody [EPR2891(2)] (AB155106)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein runs at an apparent higher MW on SDS PAGE due to glycosylation.</p>" } } }
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Sequence info

[{"sequence":"YSNFRKSMDSVGRKQYQVQHGSCSYTFLLPETDNCRSSSSPYVSNAVQRDAPLDYDDSVHRLQVLESIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLDMEDKHIVQLQSIKEEKDQLQVLVSKQNSIIEELERQLVTATVNNSALQKQQHDLTETVHSLLTMIATSSSAKSFAAKEEQITFRDCADVFRSGLTTSGIYTLTFPNSTEETKAYCDMETGGGGWTTIQRREDGSVDFQRTWKEYKVGFGNPSGEHWLGNEFVSQLTNQQRYVLKIHLRDWEGNEAYSLYEHFYLSSEEFNYRIHLKGLTGTAGKISSISQPGNDFSTKDADNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF","proteinLength":"Fragment","predictedMolecularWeight":"57.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":496,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":null,"tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Angiopoietin-2 also known as ANG2 or ANGPT2 is a glycoprotein with a molecular mass of approximately 70 kDa. It plays an important role in angiogenesis facilitating blood vessel remodeling and maturation. The protein is mainly expressed in endothelial cells where it functions by binding to the Tie2 receptor partially disrupting the Angiopoietin-Tie2 signaling axis. This disruption modulates vascular stability and permeability. The lyophilized form of the ANG-2 protein is often used in research for functional assays like ANG-2 ELISA to quantify its levels in various biological samples.
Biological function summary

Human angiogenesis processes are significantly affected by Angiopoietin-2 which serves as an antagonist to Angiopoietin-1 in regulating vascular dynamics. Although it does not form a stable complex by itself ANG2 activity is closely linked to its interactions with the Tie2 receptor modulating signal pathways that govern endothelial cell survival and permeability. Because of its dual role either stabilizing or destabilizing blood vessels under different conditions ANG2 serves as an important regulator during both normal physiological events and pathophysiological conditions.

Pathways

Angiopoietin-2 significantly contributes to the angiogenesis signaling network. It is deeply involved in the VEGF (Vascular Endothelial Growth Factor) pathway where ANG2 acts to balance VEGF activities in angiogenesis and vessel permeability. The interplay between Angiopoietin-2 and other proteins such as Angiopoietin-1 which stabilizes the vessels is vital for the precise regulation of blood vessel homeostasis during normal and disturbed conditions.

Angiopoietin-2 has a prominent role in the pathology of diseases like cancer and diabetic retinopathy. In cancer excessive ANG2 expression may lead to increased tumor angiogenesis and metastasis making it a target for cancer therapies. In diabetic retinopathy elevated levels of ANG2 correlate with vascular leakage contributing to disease progression. The relationship between Angiopoietin-1 and Angiopoietin-2 in these conditions emphasizes their collective impact on vascular anomalies associated with these disorders.
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Specifications

Form

Liquid

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General info

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Product protocols

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Target data

See full target information Angiopoietin-2
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