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Recombinant Rabbit IL-2 Protein (Active) is a Rabbit Full Length protein, in the 21 to 153 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.

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Images

Mass Spectrometry - Recombinant Rabbit IL-2 Protein (Active) (AB307411), expandable thumbnail
  • SDS-PAGE - Recombinant Rabbit IL-2 Protein (Active) (AB307411), expandable thumbnail
  • Biological Activity - Recombinant Rabbit IL-2 Protein (Active) (AB307411), expandable thumbnail
  • HPLC - Recombinant Rabbit IL-2 Protein (Active) (AB307411), expandable thumbnail

Key facts

Purity
>=95% HPLC
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Tags
Tag free
Applications
HPLC, MS, SDS-PAGE
Biologically active
Yes

Amino acid sequence

A P T S S S T K E T Q E Q L D Q L L L D L Q V L L K G V N D Y K N S K L S R M L T F K F Y M P K K V T E L K H L Q C L E E E L K P L E E V L N L A Q G K N S H G G N T R E S I S N I N V T V L K L K G S E T F M C E Y D E T V T I V E F L N R W I T F C Q S I I S A S S S

Reactivity data

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.

Alternative names

Recommended products

Recombinant Rabbit IL-2 Protein (Active) is a Rabbit Full Length protein, in the 21 to 153 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.

Key facts

Purity
>=95% HPLC
Endotoxin level
<= 0.005 EU/µg
Expression system
HEK 293 cells
Applications
HPLC, MS, SDS-PAGE
Biological activity
Fully biologically active determined by the dose dependent proliferation of CTLL-2 cells. ED50 is ≤ 1.35 ng/ml, corresponding to a specific activity of 7.4 x 105 units/mg.
Accession
O77620-1
Animal free
Yes
Species
Rabbit
Reconstitution
Reconstitute in PBS
Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

A P T S S S T K E T Q E Q L D Q L L L D L Q V L L K G V N D Y K N S K L S R M L T F K F Y M P K K V T E L K H L Q C L E E E L K P L E E V L N L A Q G K N S H G G N T R E S I S N I N V T V L K L K G S E T F M C E Y D E T V T I V E F L N R W I T F C Q S I I S A S S S
Accession
O77620
Protein length
Full Length
Predicted molecular weight
15.16 kDa
Amino acids
21 to 153
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

SDS-PAGE >= 95%

General info

Function

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.

Sequence similarities

Belongs to the IL-2 family.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Mass Spectrometry - Recombinant Rabbit IL-2 Protein (Active) (ab307411), expandable thumbnail

    Mass Spectrometry - Recombinant Rabbit IL-2 Protein (Active) (ab307411)

    Mass determination by ESI-TOF. Predicted MW is 15162.36 Da (+/-10 Da by ESI-TOF). Observed MW is 15162.91 Da.

  • SDS-PAGE - Recombinant Rabbit IL-2 Protein (Active) (ab307411), expandable thumbnail

    SDS-PAGE - Recombinant Rabbit IL-2 Protein (Active) (ab307411)

    SDS-PAGE analysis of ab307411

  • Biological Activity - Recombinant Rabbit IL-2 Protein (Active) (ab307411), expandable thumbnail

    Biological Activity - Recombinant Rabbit IL-2 Protein (Active) (ab307411)

    Fully biologically active determined by the dose dependent proliferation of CTLL-2 cells. ED50 is ≤ 1.35 ng/ml, corresponding to a specific activity of 7.4 x 10^5 units/mg.

  • HPLC - Recombinant Rabbit IL-2 Protein (Active) (ab307411), expandable thumbnail

    HPLC - Recombinant Rabbit IL-2 Protein (Active) (ab307411)

    HPLC analysis of ab307411

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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