Recombinant rabbit TIGIT protein (Fc Chimera Active) is a Fragment protein, in the 16 to 142 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.
>95% SDS-PAGE
< 1 EU/µg
HEK 293 cells
Fc tag C-Terminus
SDS-PAGE, FuncS, ELISA
Yes
A P L L A S G T M A S M I V T T G N I S A E E G G S A I L Q C H L S S T T T E V T Q V N W E Q Q D R L L A V R H V D L G W H I S P A F K E R V V P G P S L S L T L L A L T V N D T G E Y F C T Y H T Y P D G I Y K G R I F L E V R G S S V A E H S I G F Q I P
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Recombinant rabbit TIGIT protein (Fc Chimera Active) is a Fragment protein, in the 16 to 142 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, ELISA.
>95% SDS-PAGE
< 1 EU/µg
HEK 293 cells
Fc tag C-Terminus
SDS-PAGE, FuncS, ELISA
Yes
Measured by its binding ability in a functional ELISA. Immobilized Human Nectin-2, His Tag at 5 μg/ml (100 μl/well) can bind ab224645 with a linear range of 5-78 ng/ml.
Measured by its binding ability in a BLI assay. Loaded ab224645 on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.21 μM as determined in BLI assay.
No
Reconstitute at 400 µg/mL in water
pH: 7.4
Constituents: 5% Trehalose, 0.87% Sodium chloride, 0.75% Glycine, 0.61% Tris, 0.44% L-Arginine
A P L L A S G T M A S M I V T T G N I S A E E G G S A I L Q C H L S S T T T E V T Q V N W E Q Q D R L L A V R H V D L G W H I S P A F K E R V V P G P S L S L T L L A L T V N D T G E Y F C T Y H T Y P D G I Y K G R I F L E V R G S S V A E H S I G F Q I P
Fragment
40.4 kDa
16 to 142
Recombinant
Fc tag C-Terminus
Lyophilized
Blue Ice
-20°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
This product is stable after storage at:
This supplementary information is collated from multiple sources and compiled automatically.
TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.
The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.
TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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Terms & Conditions.
Immobilized human Nectin-2, His Tag at 5 μg/ml (100 μl/well) can bind ab224645 with a linear range of 5-78 ng/ml.
Loaded ab224645 on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.21 μM as determined in BLI assay.
ab224645 analyzed by SDS-PAGE and stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
As a result of glycosylation, the protein migrates as 45-50 kDa under reducing conditions, and 90-116 kDa under non-reducing conditions.
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