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AB225593

Recombinant Rabies virus (Strain India) (RABV) G protein (Tagged)

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Recombinant Rabies virus (Strain India) (RABV) G protein (Tagged) is a Rabies virus India Fragment protein, in the 20 to 459 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Glycoprotein, G

1 Images
SDS-PAGE - Recombinant Rabies virus (Strain India) (RABV) G protein (Tagged) (AB225593)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Rabies virus (Strain India) (RABV) G protein (Tagged) (AB225593)

Discontinuous SDS-PAGE (Tris-Glycine gel) (reduced) with 5% enrichment gel and 15% separation gel

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

A3RM22

Animal free

No

Carrier free

No

Species

Rabies virus India

Storage buffer

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KFPIYTIPDKLGPWSPIDIHHLSCPNNLVVEDEGCTNLSGFSYMELKVGYISAIKVNGFTCTGVVTEAETYTNFVGYVTTTFKRKHFRPTPDACRAAYNWKMAGDPRYEESLHNPYPDYHWLRTVKTTKESLVIISPSVADLDPYDKSLHSRVFPSGKCSGITISSTYCSTNHDYTIWMPENPRLGTSCDIFTNSRGKRASKGGKTCGFVDERGLYKSLKGACKLKLCGVLGLRLMDGTWVAMQTSDETKWCPPDQLVNLHDFRSDEIEHLVVEELVKKREECLDALESIMATKSVSFRRLSHLRKLVPGFGKAYTIFNKTLMEADAHYKSVRTWNEIIPSKGCLRVGGRCHPHVNGVFFNGIILGPDGHVLIPEMQSSLLQQHMELLESSVIPLMHPLADPSTVFKDGDEAEDFVEVHLPDVHKQISGVDLGLPSWGKY","proteinLength":"Fragment","predictedMolecularWeight":"65.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":459,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"A3RM22","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Rabies virus (Strain India) glycoprotein (G protein) is an important component of the virus structure facilitating its attachment to host cells. It is a type I transmembrane protein with a mass of approximately 65 kDa. This glycoprotein locates predominantly on the surface of the rabies virion. It's responsible for initiating viral infection by binding to cellular receptors on the host surface playing an important role in viral entry and host-cell fusion.
Biological function summary

The glycoprotein G participates actively in the virions' ability to infect host cells by mediating the adsorption of the virus to the cell membrane which leads to membrane fusion. This protein exists as a trimer on the viral envelope and functions in concert with other viral proteins. Additionally the G protein is immunogenic inducing production of neutralizing antibodies making it a target for vaccine development.

Pathways

G protein's biological function is integral to the viral life cycle particularly in pathways related to host cell entry and immune evasion. It interacts with several host proteins including nicotinic acetylcholine receptor and neural cell adhesion molecule (NCAM) to facilitate viral entry. Furthermore it plays a role in the evasion of the host's immune system by gradually changing its surface epitopes.

Rabies virus glycoprotein G is central to the pathogenesis of rabies a neurodegenerative disease with a high case-fatality rate if untreated. This protein is important in the development of the rabies vaccine strategies and is a major target for neutralizing antibodies. During rabies infection G protein modulates interaction with host immune proteins which is relevant for understanding viral pathogenesis and the development of new treatments.
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Specifications

Form

Liquid

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General info

Function

Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1 (By similarity). In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).

Sequence similarities

Belongs to the lyssavirus glycoprotein family.

Post-translational modifications

Glycosylated and palmitoylated by host. Glycosylation is crucial for glycoprotein export at the cell surface (By similarity).

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Product protocols

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Target data

Attaches the virus to host cellular receptor, inducing endocytosis of the virion by using different host proteins including TFRC, GRM2 and ITGB1 (By similarity). In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein and thereby facilitate rabies virus entry into cells (By similarity).
See full target information G

Additional targets

Rabies virus (Strain India) (RABV) G
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