Recombinant rat BTK protein is a Full Length protein, in the 1 to 660 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A A V I L E S I F L K R S Q Q K K K T S P L N F K K R L F L L T V H K L S Y Y E Y D F E R G R R G S K K G S I D V E K I T C V E T V V P E K N P P P E R Q I P V P R G E E S S E M E Q I S I I E R F P Y P F Q V V Y D E G P L Y V F S P T E E L R K R W I H Q L K N V I R Y N S D L V Q K Y H P C F W I D G Q Y L C C S Q T A K N A M G C Q I L E N R N G S L K P G S S H R K T K K P L P P T P E E D Q I L K K P L P P E P T A A P I S T S E L K K V V A L Y D Y M P M N A N D L Q L R K G E E Y F I L E E S N L P W W R A R D K N G Q E G Y I P S N Y V T E A E D S I E M Y E W Y S K H M T R S Q A E Q L L K Q E G K E G G F I V R D S S K A G K Y T V S V F A K S T G E P Q G V I R H Y V V C S T P Q S Q Y Y L A E K H L F S T I P E L I N Y H Q H N S A G L I S R L K Y P V S K Q N K N A P S T A G L G Y G S W E I D P K D L T F L K E L G T G Q F G V V K Y G K W R G Q Y D V A I K M I R E G S M S E D E F I E E A K V M M N L S H E K L V Q L Y G V C T K Q R P I F I I T E Y M A N G C L L N Y L R E M R H R F Q T Q Q L L E M C K D V C E A M E Y L E S K Q F L H R D L A A R N C L V N D Q G V V K V S D F G L S R Y V L D D E Y T S S V G S K F P V R W S P P E V L M Y S K F S S K S D I W A F G V L M W E I Y S L G K I P Y E R F T N S E T A E H I A Q G L R L Y R P H L A S D R V Y T I M Y S C W H E K A D E R P S F K I L L S N I L D V M D E E S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant rat BTK protein is a Full Length protein, in the 1 to 660 aa range, expressed in Baculovirus infected Sf9, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab207956 was found to be 66 nmol/min/mg.
- Animal free
- No
- Concentration
- Storage buffer
pH: 7.5
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M A A V I L E S I F L K R S Q Q K K K T S P L N F K K R L F L L T V H K L S Y Y E Y D F E R G R R G S K K G S I D V E K I T C V E T V V P E K N P P P E R Q I P V P R G E E S S E M E Q I S I I E R F P Y P F Q V V Y D E G P L Y V F S P T E E L R K R W I H Q L K N V I R Y N S D L V Q K Y H P C F W I D G Q Y L C C S Q T A K N A M G C Q I L E N R N G S L K P G S S H R K T K K P L P P T P E E D Q I L K K P L P P E P T A A P I S T S E L K K V V A L Y D Y M P M N A N D L Q L R K G E E Y F I L E E S N L P W W R A R D K N G Q E G Y I P S N Y V T E A E D S I E M Y E W Y S K H M T R S Q A E Q L L K Q E G K E G G F I V R D S S K A G K Y T V S V F A K S T G E P Q G V I R H Y V V C S T P Q S Q Y Y L A E K H L F S T I P E L I N Y H Q H N S A G L I S R L K Y P V S K Q N K N A P S T A G L G Y G S W E I D P K D L T F L K E L G T G Q F G V V K Y G K W R G Q Y D V A I K M I R E G S M S E D E F I E E A K V M M N L S H E K L V Q L Y G V C T K Q R P I F I I T E Y M A N G C L L N Y L R E M R H R F Q T Q Q L L E M C K D V C E A M E Y L E S K Q F L H R D L A A R N C L V N D Q G V V K V S D F G L S R Y V L D D E Y T S S V G S K F P V R W S P P E V L M Y S K F S S K S D I W A F G V L M W E I Y S L G K I P Y E R F T N S E T A E H I A Q G L R L Y R P H L A S D R V Y T I M Y S C W H E K A D E R P S F K I L L S N I L D V M D E E S
- Protein length
- Full Length
- Predicted molecular weight
- 105 kDa
- Amino acids
- 1 to 660
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BTK or Bruton's tyrosine kinase is an important enzyme in the body that functions mainly in signaling pathways within cells. It is known for its involvement in the development and activation of B cells and is expressed in cells of the hematopoietic system. The BTK protein has a molecular weight of about 76 kDa. Researchers can study BTK using tools like the BTK ELISA kit which helps measure its presence in biological samples. Furthermore there are specific anti-BTK antibodies which assist in the detection of BTK bands during analysis. BTK inhibitors like BTK C481S have been developed to regulate its activity.
- Biological function summary
BTK plays an important role in the immune response by transmitting signals from the B cell receptor to the inside of the cell which promotes B cell maturation and survival. It operates as part of a larger signal transduction complex that includes other proteins and molecules. For accurate quantification of BTK expression a BTK sandwich ELISA kit may be utilized capturing and revealing the BTK protein's presence in sample preparations.
- Pathways
BTK associates with both the B cell receptor signaling and the PI3K-Akt pathway. These pathways are critical for the proper functioning and proliferation of B cells. BTK interacts with proteins like PLCγ2 and BLNK in the signaling cascade highlighting its central role in transmitting extracellular signals to elicit appropriate cellular responses.
- Associated diseases and disorders
BTK is closely linked to X-linked agammaglobulinemia (XLA) a genetic condition marked by an absence of mature B cells. This highlights the connection between BTK function and immune competence. Moreover aberrant BTK activity has been implicated in some types of B cell malignancies such as chronic lymphocytic leukemia (CLL). The BTK pathway's dysregulation in these disorders may involve interactions with proteins like SYK suggesting that targeting BTK could offer therapeutic benefits in managing such conditions.
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2 product images
SDS-PAGE - Recombinant rat BTK protein (ab207956)
SDS-PAGE analysis of ab207956.
Functional Studies - Recombinant rat BTK protein (ab207956)
Sample Kinase Activity assay plot using ab207956 showing the specific activity to be 66 nmol/min/mg.
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