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AB283449

Recombinant rat CNTF protein (Active)

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Recombinant rat CNTF protein (Active) is a Rat Full Length protein, in the 2 to 200 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

View Alternative Names

Ciliary neurotrophic factor, CNTF, Cntf

4 Images
Mass Spectrometry - Recombinant rat CNTF protein (Active) (AB283449)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant rat CNTF protein (Active) (AB283449)

Mass determination by ESI TOF

Predicted mass is 22779.96 +/- 10Da by ESI TOF. Observed mass is 22780.53 +/- 10Da

Functional Studies - Recombinant rat CNTF protein (Active) (AB283449)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat CNTF protein (Active) (AB283449)

Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤0.114 μg/ml, corresponding to a specific activity of 8.8 x 103 units/mg.

SDS-PAGE - Recombinant rat CNTF protein (Active) (AB283449)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat CNTF protein (Active) (AB283449)

SDS-PAGE analysis of ab283449.

HPLC - Recombinant rat CNTF protein (Active) (AB283449)
  • HPLC

Supplier Data

HPLC - Recombinant rat CNTF protein (Active) (AB283449)

HPLC analysis of ab283449.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

FuncS, HPLC, Mass Spec, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤0.114 μg/ml, corresponding to a specific activity of 8.8 x 103 units/mg.

Accession

P20294

Animal free

Yes

Carrier free

No

Species

Rat

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"AFAEQTPLTLHRRDLCSRSIWLARKIRSDLTALMESYVKHQGLNKNINLDSVDGVPVASTDRWSEMTEAERLQENLQAYRTFQGMLTKLLEDQRVHFTPTEGDFHQAIHTLMLQVSAFAYQLEELMVLLEQKIPENEADGMPATVGDGGLFEKKLWGLKVLQELSQWTVRSIHDLRVISSHQMGISALESHYGAKDKQM","proteinLength":"Full Length","predictedMolecularWeight":"22.78 kDa","actualMolecularWeight":"22.78 kDa","aminoAcidEnd":200,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20294","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

Geater than 95% purity by HPLC

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General info

Function

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Sequence similarities

Belongs to the CNTF family.

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Product protocols

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Target data

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
See full target information Cntf
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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