Recombinant rat CNTF protein (Active) is a Rat Full Length protein, in the 2 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Images
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
Amino acid sequence
A F A E Q T P L T L H R R D L C S R S I W L A R K I R S D L T A L M E S Y V K H Q G L N K N I N L D S V D G V P V A S T D R W S E M T E A E R L Q E N L Q A Y R T F Q G M L T K L L E D Q R V H F T P T E G D F H Q A I H T L M L Q V S A F A Y Q L E E L M V L L E Q K I P E N E A D G M P A T V G D G G L F E K K L W G L K V L Q E L S Q W T V R S I H D L R V I S S H Q M G I S A L E S H Y G A K D K Q M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Alternative names
Ciliary neurotrophic factor, CNTF, Cntf
Recommended products
Recombinant rat CNTF protein (Active) is a Rat Full Length protein, in the 2 to 200 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC.
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤0.114 μg/ml, corresponding to a specific activity of 8.8 x 103 units/mg.
- Accession
- P20294-1
- Animal free
- Yes
- Species
- Rat
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- A F A E Q T P L T L H R R D L C S R S I W L A R K I R S D L T A L M E S Y V K H Q G L N K N I N L D S V D G V P V A S T D R W S E M T E A E R L Q E N L Q A Y R T F Q G M L T K L L E D Q R V H F T P T E G D F H Q A I H T L M L Q V S A F A Y Q L E E L M V L L E Q K I P E N E A D G M P A T V G D G G L F E K K L W G L K V L Q E L S Q W T V R S I H D L R V I S S H Q M G I S A L E S H Y G A K D K Q M
- Accession
- P20294
- Protein length
- Full Length
- Predicted molecular weight
- 22.78 kDa
- Actual molecular weight
- 22.78 kDa
- Amino acids
- 2 to 200
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Geater than 95% purity by HPLC
General info
- Function
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
- Sequence similarities
Belongs to the CNTF family.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
SDS-PAGE - Recombinant rat CNTF protein (Active) (ab283449)
SDS-PAGE analysis of ab283449.
Mass Spectrometry - Recombinant rat CNTF protein (Active) (ab283449)
Mass determination by ESI TOFPredicted mass is 22779.96 +/- 10Da by ESI TOF. Observed mass is 22780.53 +/- 10Da
HPLC - Recombinant rat CNTF protein (Active) (ab283449)
HPLC analysis of ab283449.
Functional Studies - Recombinant rat CNTF protein (Active) (ab283449)
Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤0.114 μg/ml, corresponding to a specific activity of 8.8 x 103 units/mg.
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