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AB219422

Recombinant rat Cyclophilin F protein (Active)

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Recombinant rat Cyclophilin F protein (Active) is a Rat Full Length protein, in the 29 to 206 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS, Mass Spec.

View Alternative Names

PPIase F, Cyclophilin D, Cyclophilin F, Rotamase F, CyP-D, CypD, Ppif

1 Images
SDS-PAGE - Recombinant rat Cyclophilin F protein (Active) (AB219422)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat Cyclophilin F protein (Active) (AB219422)

15% SDS-PAGE - Recombinant rat Cyclophilin F protein (Active) (ab219422) at 3 μg.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmol of suc-AAPF-PNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.

Accession

P29117

Animal free

No

Carrier free

No

Species

Rat

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSCSDGGARGANSSSQNPLVYLDVGADGQPLGRVVLELKADVVPKTAENFRALCTGEKGFGYKGSTFHRVIPAFMCQAGDFTNHNGTGGKSIYGSRFPDENFTLKHVGPGVLSMANAGPNTNGSQFFICTIKTDWLDGKHVVFGHVKEGMDVVKKIESFGSKSGKTSKKIVITDCGQLS","proteinLength":"Full Length","predictedMolecularWeight":"21.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":206,"aminoAcidStart":29,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P29117","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography techniques.

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General info

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (By similarity). Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (PubMed : 19228691).

Sequence similarities

Belongs to the cyclophilin-type PPIase family.

Post-translational modifications

Acetylated at Lys-166; deacetylated at Lys-166 by SIRT3.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (By similarity). Involved in regulation of the mitochondrial permeability transition pore (mPTP) (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels (PubMed : 8567677, PubMed : 9309684, PubMed : 9820802). Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (PubMed : 19228691).
See full target information Peptidyl-prolyl cis-trans isomerase F, mitochondrial
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